The process of embryo abortion of stenospermocarpic grape and it develops into plantlet in vitro using embryo rescue

Li, Shasha; Liu, Keke; Yu, Saisai; Jia, Shanshan; Chen, Shuo; Fu, Yuheng; Feng, Sun; Luo, Qiong; Wang, Yuejin

doi:10.1007/s11240-020-01926-y

Cited by 22 publications

(24 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our study, germination ranged from 31.00% to 89.74% (Table 4). These results are comparable or superior to other values reported in the literature (Li et al, 2014(Li et al, , 2020bLiu et al, 2008;Zhu et al, 2020). Among the eight crosses tested, GE1972 and GE1974 had the greatest percentage of normal plantlets (40.44% and 43.59%, respectively).…”

Section: Discussionsupporting

confidence: 85%

“…Our study did not investigate embryo development specifically to reduce the handling of extracted embryos from the ovules. Based on the literature, many dates are proposed as optimal sampling times, varying from 39 to 70 d after flowering (Bharathy et al, 2003;Gray et al, 1990;Li et al, 2014Li et al, , 2015aLi et al, , 2020bLiu et al, 2008;Zhu et al, 2020). GDD can be a viable predictor for sampling time.…”

Section: Discussionmentioning

confidence: 99%

“…In Minnesota, this is the week leading up to veraison. This sampling time point may coincide with embryo development that is unsuitable for ovule culture or possible hormonal regulation or signaling associated with berry softening and ripening at the end of the lag phase (Keller, 2020;Li et al, 2020b).…”

Section: Discussionmentioning

confidence: 99%

“…Table grape breeding programs have carried out hybridizations to obtain seedless cultivars to respond to market demand. Grape breeders have crossed seedless pollen donors with seeded mother plants for decades to get a proportion of seedless offspring each generation to advance cultivar development (Gray et al, 1990;Li et al, 2020b). In some seeded Â seedless crosses, less than 10% of the F 1 progeny are seedless; however, using two seedless parents, this value can reach 82% to 100% (Bharathy et al, 2005;Emershad et al, 1989;Ji et al, 2013;Ramming, 1990;Tian and Wang, 2008;Zhu et al, 2020).…”

mentioning

confidence: 99%

“…The developmental progression through in vitro culture of an ovule to a normal seedling is influenced by genotype, cultural conditions, and embryo development in the artificial environment (Li et al, 2015b). Various nutrient media have been tested in grapes (Gray et al, 1987), sampling time after flowering (Gray et al, 1990;Li et al, 2020b;Pommer et al, 1995), and length of ovule incubation as summarized by Li et al (2015b). Selecting the germination media on which embryos are cultured can affect the development into normal plantlets or myriad aberrations (Ji et al, 2013).…”

mentioning

confidence: 99%

See 4 more Smart Citations

Embryo Rescue of Cold-hardy Table Grapes

Moreira¹,

Clark²

2021

horts

View full text Add to dashboard Cite

Seedlessness is one of the most desirable traits for table and raisin grapes. Stenospermocarpic cultivars are desirable because they have large berries with superior quality. Embryo rescue techniques have been widely used to get progeny seedling populations from crosses using seedless mother plants. Selection of the female parent, sampling time, and the growth medium are the most crucial to the success of this technique. This study investigated the effect of best sampling time and media composition on embryo rescue efficiency in a cold-hardy hybrid grape breeding program. We sampled ovules 5 to 9 weeks after flowering, and we tested four media compositions. The greatest percentages of embryo germination and normal seedlings were obtained when ovules were harvest at 8 weeks after flowering, indicating that it is suitable to harvest ovules at veraison, when the extraction of ovules is easier as a result of softer berry flesh. For the media composition experiment, all ovules were harvested at 8 weeks after flowering. Nitsch & Nitsch culture medium had very low germination, and the resulting seedlings performed the lowest compared with the other treatments. Lloyd & McCown Woody Plant Basal Medium (WPM) increased the number of embryos germinated significantly, and a number of normal seedlings and plantlets developed. Although there was no significant difference among the other three media containing WPM supplemented with different doses of plant regulators, the WPM Plus medium [with cytokinin (6-benzlaminopurine), indole-3-butyric acid, gibberellin, and casein hydrolysate] promoted the greatest percentage of established plants (46.98%). Therefore, the 8-weeks-after-flowering harvest time and the WPM Plus medium were selected for use in the embryo rescue protocol at the University of Minnesota grape breeding program.

show abstract

Section: Discussionsupporting

confidence: 85%