The problem of polyspermy in in vitro fertilization

Wentz, Anne Colston; Repp, John E.; Maxson, Wayne S.; Pittaway, Donald E.; Torbit, C. A.

doi:10.1016/s0015-0282(16)47474-1

Cited by 56 publications

(20 citation statements)

References 18 publications

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“…Inhibition or delay of second polar body extrusion and formation of two female pronuclei (or possibly one diploid) together with a single sperm pronucleus (monospermic digyny) (Palermo et al, 1995). Polyspermy may be correlated with other factors, including oocyte immaturity and postmaturity (Wentz et al, 1983), sperm concentration (Wolf et al, 1984), inherent defects or fractures of the Fig. 10.…”

Section: Discussionmentioning

confidence: 99%

“…zona pellucida occurring during oocyte collection (Wentz et al, 1983), progesterone levels in the follicular fluid (Ben-Rafael et al, 1987), and more recently, high serum estradiol levels (Andrea et al, 2000). In natural conception, it has been reported that triploid embryos may proceed to term, and result in a liveborn child (Uchida and Freeman, 1985).…”

Section: Discussionmentioning

confidence: 99%

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Comparative evaluation of human embryonic stem cell lines derived from zygotes with normal and abnormal pronuclei

Qin¹,

Gao²,

Wang³

et al. 2010

Developmental Dynamics

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Human embryonic stem (hES) cell lines have been derived from normally or abnormally fertilized zygotes. However, the similar and different properties of these two types of hES cell lines are not well-known. To address this question, we generated nine hES cell lines from zygotes containing normal (2PN) and abnormal (0PN, 1PN, 3PN) pronuclei. A side-by-side comparison showed that all cell lines exhibited distinct identity and karyotypical stability. They expressed similar ''stemness'' markers and alkaline phosphatase activity and differentiated into three embryonic germ lineages in embryoid bodies and teratomas. Under neural differentiation-promoting conditions, they were directed into neural progenitors and neurons. However, a variation in cell cycle and the relative abundance of gene expression of undifferentiated and differentiated markers were observed. These variations were also seen among individually derived normal hES cell lines. Thus, normal hES cell lines can be developed from fertilized zygotes with abnormal pronuclei usually excluded from clinical use. Developmental Dynamics 239:425-438,

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Comparative evaluation of human embryonic stem cell lines derived from zygotes with normal and abnormal pronuclei

Qin¹,

Gao²,

Wang³

et al. 2010

Developmental Dynamics

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“…In the last decade multipronuclear eggs have been widely used in human in-vitro fertilization (IVF) for experimental purposes (Wentz et al, 1983;Van Blerkom et al, 1984;Kola et al, 1987). Cytogenetic evidence has shown that zygotes having three pronuclei usually arise through dispermic fertilization and at the first cleavage contain one maternal and two paternal genomes (Rudak et al, 1984;Angell et al, 1986).…”

Section: Introductionmentioning

confidence: 99%

Fertilization and early embryology: The chromosomal complements of multipronuclear human zygotes resulting from intracytoplasmic sperm injection

et al. 1996

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Implementation of intracytoplasmic sperm injection (ICSI)in human in-vitro fertilization (IVF) has highlighted the need for information about the risk of nuclear spindle damage caused by this procedure. For this purpose we studied the final products of oocyte meiosis at the first cleavage division of multipronuclear zygotes arising after ICSI, and compared the results with abnormally fertilized oocytes after conventional in-vitro insemination. Of 37 successfully analysed tripronuclear zygotes, 18 had three individual metaphases. Abnormal complements of 11 zygotes in this group indicated that non-disjunction occurred predominantly at the second meiotic division of the oocytes. Nine of the 37 tripronuclear zygotes exhibited two individual metaphases. Seven were abnormal and there were some indications that non-disjunction took place during oocyte meiosis. Of the 37 tripronuclear zygotes, 10 had a single metaphase and three showed an aneuploid number of chromosomes. The overall rate of aneuploidy among tripronuclear microinjected zygotes was 56.7%. In addition, seven zygotes with more than three pronuclei arising after ICSI displayed severely depleted chromosome complements. The incidence of non-disjunction in oocytes fertilized by conventional in-vitro insemination was significantly lower (20.0%, P < 0.01), since only four zygotes had an aneuploid number of chromosomes. Our findings suggest that ICSI might interfere with regular chromosome segregation at the second meiotic division of the oocytes.

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“…1984) that, in the case of the diploid embryos, if the rejected pronucleus was the female pronucleus, then a possible mechanism for hydatidiform mole forma tion existed. In view of the potential pathological consequences of polyspermy, some IVF groups now strongly advocate examination of the pronuclei so that dispcrmic oocytes can be rejected (Wentz et al, 1983: Blerkom et al. 1984: Rudak ct al., 1984.…”

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confidence: 99%

Consequences of polyspermy in man

Angell

Templeton

Messinis

1986

Cytogenet Genome Res

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Polyspermy occurs with a frequency of about 12% under some hormonal regimens currently being used for in vitro fertilization and embryo transfer. Oocytes with three pronuclei usually show normal cleavage and are morphologically indistinguishable from embryos produced by normal fertilization. Chromosome studies and DNA measurements on dispermic embryos show that about one-half are triploid, the others having either a normal diploid chromosome complement or a severely depleted chromosome complement. The evidence suggests that these errors must arise at the first mitotic division of the oocyte. Aberrant spindle formation is implicated.

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The problem of polyspermy in in vitro fertilization

Cited by 56 publications

References 18 publications

Comparative evaluation of human embryonic stem cell lines derived from zygotes with normal and abnormal pronuclei

Comparative evaluation of human embryonic stem cell lines derived from zygotes with normal and abnormal pronuclei

Fertilization and early embryology: The chromosomal complements of multipronuclear human zygotes resulting from intracytoplasmic sperm injection

Consequences of polyspermy in man

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