The Probiotic VSL#3 Has Anti-inflammatory Effects and Could Reduce Endoscopic Recurrence After Surgery for Crohn's Disease

Fedorak, Richard N.; Feagan, Brian G.; Hotte, Naomi; Leddin, Des; Dieleman, Levinus A.; Petrunia, Denis M.; Enns, Robert; Bitton, Alain; Chiba, Naoki; Paré, Pierre; Rostom, Alaa; Marshall, John K.; Depew, William T.; Bernstein, Çharles N.; Panaccione, Remo; Aumais, Guy; Steinhart, A. Hillary; Cockeram, Alan; Bailey, Robert J.; Gionchetti, Paolo; Wong, Cindy J.; Madsen, Karen

doi:10.1016/j.cgh.2014.10.031

Cited by 192 publications

(132 citation statements)

References 29 publications

Supporting

Mentioning

116

Contrasting

Unclassified

Order By: Relevance

“…The beneficial role of LP in inducing IBD remission has been highly appreciated by accumulating studies [29, 30]. Ours and other groups have found that SLP plays a crucial part in controlling inflammation, maintaining healthy gut microbiota and protecting the intestinal barrier [12, 31].…”

Section: Discussionmentioning

confidence: 99%

Micro Integral Membrane Protein (MIMP), a Newly Discovered Anti-Inflammatory Protein of Lactobacillus Plantarum, Enhances the Gut Barrier and Modulates Microbiota and Inflammatory Cytokines

Yin¹,

Yan

Weng³

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Recent studies have demonstrated that the manipulation of the gut microbiome represents a promising treatment for inflammatory bowel disease (IBD). We previously identified micro integral membrane protein (MIMP) as the smallest domain of surface layer protein from Lactobacillus Plantarum. However, the therapeutic relevance of MIMP in IBD remains unknown. Methods: We initially employed a dextran sodium sulphate (DSS)-induced colitis model and evaluated the effect of MIMP on the inflammation response, intestinal barrier and gut microbiota using histological examination, Fluorescein isothiocyanate-Dextran detection and pyrosequencing analysis respectively. We then established peripheral blood mononuclear cells (PBMCs) and an epithelial CaCO-2 co-culture model to investigate the regulatory role of MIMP in inflammatory cytokines. The level changes of inflammatory cytokines were detected using Enzyme-linked immunosorbent and real-time polymerase chain reaction assay. The involved regulatory mechanisms were investigated mainly using dual luciferase reporter and chromatin immunoprecipitation assay. Results: In the DSS-induced colitis model, we observed that MIMP intervention effectively improved the body weight loss, increased the colon length and decreased disease activity index. Consistently, the inflammation scores in the MIMP treatment group were significantly lower than those in the DSS treatment group. Furthermore, MIMP intervention was found to successfully neutralize DSS treatment by decreasing the expression of pro-inflammatory cytokines (IFN-γ, IL-17 and IL-23) and increasing the expression of anti-inflammatory cytokines (IL-4 and IL-10). Notably, the permeability assay demonstrated that the MIMP treatment group was remarkably lower than that in the DSS treatment group. We also showed that MIMP improved gut microbiota dysbiosis caused by DSS-induced inflammation. Additionally, in PBMCs and the CaCO-2 co-culture model, MIMP showed an obvious suppressive effect on lipopolysaccharide-induced inflammation in a time- and dose-dependent manner. Furthermore, we revealed that MIMP could modulate inflammatory cytokine expression through the toll-like receptor 4 pathway and histone acetylation. Conclusions: Our results suggested that MIMP showed a significant anti-inflammatory effect through regulating the gut barrier, microbiota and inflammatory cytokines. MIMP may have translational relevance as clinically relevant therapy for IBD patients.

show abstract

Section: Discussionmentioning

confidence: 99%

Micro Integral Membrane Protein (MIMP), a Newly Discovered Anti-Inflammatory Protein of Lactobacillus Plantarum, Enhances the Gut Barrier and Modulates Microbiota and Inflammatory Cytokines

Yin¹,

Yan

Weng³

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…We read the study by Fedorak et al 1 with interest. Although there have been several randomized controlled trials (RCTs) evaluating the efficacy of VSL#3 in ulcerative colitis, the only previous study in Crohn's disease post-surgery is the small study by Campieri et al 2 The optimal management of Crohn's disease post-operatively remains unclear, with conflicting evidence for the use of 5-aminosalicylates, and immunomodulator therapies, and few data for biological therapies.…”

Section: Word Count: 279mentioning

confidence: 99%

VSL#3 in Postoperative Crohn’s Disease

Manship

Ford

2015

Clinical Gastroenterology and Hepatology

View full text Add to dashboard Cite

“…The cause of Crohn's disease is still not well established. However, intestinal immune system and bacterial antigens originated from intestinal bacterial flora, or infection are incriminated to trigger such chronic inflammatory condition [29]. Many studies using single bacterial species of probiotics showed no effectiveness in preventing the recurrence of CD after surgical intervention, howe-ver, one-year consumption had noteworthy results of reducing recurrence as well as the pro-inflammatory cytokines, especially when probiotics treatment is administered before surgery rather than later [29].…”

Section: Crohn's Disease (Cd)mentioning

confidence: 99%

“…However, intestinal immune system and bacterial antigens originated from intestinal bacterial flora, or infection are incriminated to trigger such chronic inflammatory condition [29]. Many studies using single bacterial species of probiotics showed no effectiveness in preventing the recurrence of CD after surgical intervention, howe-ver, one-year consumption had noteworthy results of reducing recurrence as well as the pro-inflammatory cytokines, especially when probiotics treatment is administered before surgery rather than later [29]. Several studies revealed controversial results; two studies were conducted using Lactobacillus GG, the first one demonstrated a considerable clinical improvement in children suffering from active CD, while the other study found that the probiotics cannot prevent its relapse, with no significant statistical differences compared with placebo [30].…”

Section: Crohn's Disease (Cd)mentioning

confidence: 99%

Probiotics: Past, Present and Future Challenges

Altaha

Wadi

Shehabi

2018

int. arab. j antimicrob. agents

View full text Add to dashboard Cite

The term probiotic is utilized to describe useful living microorganisms, intended to colonize the large intestine, when consumed in adequate amounts, they confer physiological health benefits to the host. In the last decade, probiotics have been widely used as a nutritional supplement and became a rapidly developing research topic that gained importance in medicine. Recent clinical trials have found that consumption of beneficial bacterial species can manipulate gut flora and reduce or prevent the development of certain serious diseases.

show abstract

The Probiotic VSL#3 Has Anti-inflammatory Effects and Could Reduce Endoscopic Recurrence After Surgery for Crohn's Disease

Cited by 192 publications

References 29 publications

Micro Integral Membrane Protein (MIMP), a Newly Discovered Anti-Inflammatory Protein of Lactobacillus Plantarum, Enhances the Gut Barrier and Modulates Microbiota and Inflammatory Cytokines

Micro Integral Membrane Protein (MIMP), a Newly Discovered Anti-Inflammatory Protein of Lactobacillus Plantarum, Enhances the Gut Barrier and Modulates Microbiota and Inflammatory Cytokines

VSL#3 in Postoperative Crohn’s Disease

Probiotics: Past, Present and Future Challenges

Contact Info

Product

Resources

About