The probiotic properties of different preparations using Lactococcus lactis Z-2 on intestinal tract, blood and hepatopancreas in Cyprinus carpio

Wang, Junli; Feng, Junchang; Li, Shasha; Cai, Zhongliang; Song, Dongying; Yang, Liping; Nie, Guoxing

doi:10.1016/j.aquaculture.2021.736911

Cited by 20 publications

(8 citation statements)

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“…Figure S4 showed the abundance in wild and reared red cusk eel, of possible candidates to further study as probiotics, such as Lactococcus, Carnobacterium and Vagococcus. These genera are easy to culture and there are previous report supporting their use as probiotics [29,30,[57][58][59][60]. However, it must be noted that the results obtained in this study were obtained analyzing samples of whole intestinal contents, thus corresponding to the total gut microbiota.…”

Section: Discussionmentioning

confidence: 78%

Red Cusk-Eel (Genypterus chilensis) Gut Microbiota Description of Wild and Aquaculture Specimens

et al. 2022

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Chile has promoted the diversification of aquaculture and red cusk-eel (Genypterus chilensis) is one of the prioritized species. However, many aspects of the biology of the species are unknown or have little information available. These include intestinal microbiota, an element that may play an important role in the nutrition and defense of cultured animals for meat production. This study compares the microbiota composition of the intestinal contents of wild and aquaculture fish to explore the microbial communities present and their potential contribution to the host. DNA was extracted from the intestinal content samples and the V4 region of the 16S rRNA gene was amplified and sequenced using the Ion Torrent platform. After the examination of the sequences, strong differences were found in the composition at the level of phylum, being Firmicutes and Tenericutes the most abundant in aquaculture and wild condition, respectively. At the genus level, the Vagococcus (54%) and Mycoplasma (97%) were the most prevalent in the microbial community of aquaculture and wild condition, respectively. The evaluation of predicted metabolic pathways in these metagenomes showed that in wild condition there is an important presence of lipid metabolism belonging to the unsaturated fatty acid synthesis. In the aquaculture condition, the metabolism of terpenoids and polyketides were relevant. To our knowledge, this is the first study to characterize and compare the intestinal microbiota of red cusk-eel (Genypterus chilensis) of wild and aquaculture origin using high-throughput sequencing.

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Section: Discussionmentioning

confidence: 78%

Red Cusk-Eel (Genypterus chilensis) Gut Microbiota Description of Wild and Aquaculture Specimens

et al. 2022

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“…It is widely acknowledged that LDH is an important enzyme in animal cells 35 , and commonly used in environmental physiology and disease diagnosis and analysis 36 . T-AOC is a comprehensive index used to measure the functional status of the enzymatic and non-enzymatic components of the antioxidant system 37 . A study indicated that bacterial infection (e.g., Aeromonas hydrophila) decreased the activity of T-AOC in common carp 38 .…”

Section: Discussionmentioning

confidence: 99%

Safety assessment of subchronic feeding of insect-resistant and herbicide-resistant transgenic soybeans to juvenile channel catfish (Ictalurus punctatus)

Xiang

Luo

Jiang

et al. 2023

Sci Rep

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Transgenic soybean is one of the most planted crops for human food and animal feed. The channel catfish (Ictalurus punctatus) is an important aquatic organism cultured worldwide. In this study, the effect of six different soybean diets containing: two transgenic soybeans expressing different types of cp4-epsps, Vip3Aa and pat genes (DBN9004 and DBN8002), their non-transgenic parent JACK, and three conventional soybean varieties (Dongsheng3, Dongsheng7, and Dongsheng9) was investigated in juvenile channel catfish for eight weeks, and a safety assessment was performed. During the experiment, no difference in survival rate was observed in six groups. The hepatosomatic index (HSI) and condition factor (CF) showed no significant difference. Moreover, comparable feed conversion (FC), feeding rate (FR), and feed conversion ratio (FCR) were found between transgenic soybean and JACK groups. Assessment of growth performance showed that the weight gain rate (WGR) and specific growth rate (SGR) of channel catfish were consistent. In addition, there were no changes in enzyme activity indexes (lactate dehydrogenase (LDH), total antioxidant capacity (T-AOC), aspartate aminotransferase (AST) and alanine aminotransferase (ALT)) in channel catfish among treatments. The research provided an experimental basis for the aquaculture feed industry to employ transgenic soybean DBN9004 and DBN8002 for commercial purposes.

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“…L. casei, as a probiotic, can stimulate the innate immune response in fsh by increasing the expression of IL-1β and IFN-c [34]. Live L. lactis can signifcantly enhance the levels of TNF-α, IL-1β, and IL-6 in C. carpio [15]. Te cellular components of probiotics could stimulate immune-related gene expression and may be useful as adjuvants for vaccines in aquaculture [35].…”

Section: Discussionmentioning

confidence: 99%

“…Probiotics have been applied in aquaculture for several decades. Lactococcus lactis, an endogenous microbe, could exert growth performance, immune response, and disease resistance in aquaculture animals [13][14][15]. As an endogenous microbe, L. lactis could adhere to mucosal surfaces of the gut and then colonize, establish, and multiply in the fsh gut [13].…”

Section: Introductionmentioning

confidence: 99%

Construction and Evaluation of Recombinant Lactococcus lactis Expressing the vscO Gene as a Safe Live Oral Vaccine against Vibrio alginolyticus

Yang

Huang

et al. 2023

Aquaculture Research

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Vibrio alginolyticus is responsible for significant economic losses in the fish industry. It is urgent to develop a vaccine against V. alginolyticus. In this study, a recombinant Lactococcus lactis expressing the VscO protein of V. alginolyticus (Lc-pMG36e-vscO) is constructed. The Lc-pMG36e-vscO has good hereditary stability. The immune responses in pearl gentian grouper (Epinephelus fuscoguttatus (♀) × E. lanceolatu (♂)) vaccinated with Lc-pMG36e-vscO are evaluated. The titers of specific antibody in the immunized grouper displayed an upward and then downward tendency during the total experiments and reached the peak value of 9.6 at the fourth week after booster immunization. The expression levels of IL-1β, IL-16, TNF-α, and MHC-1α gene in the Lc-pMG36e-vscO group were significantly upregulated after vaccination, indicating that the Lc-pMG36e-vscO could induce an effective and durable immune response. In vivo challenge of the Lc-pMG36e-vscO-immunized fish with V. alginolyticus showed a relative survival percentage of 68%. These results indicated that Lc-pMG36e-vscO could be promising as a candidate oral vaccine against V. alginolyticus.

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The probiotic properties of different preparations using Lactococcus lactis Z-2 on intestinal tract, blood and hepatopancreas in Cyprinus carpio

Cited by 20 publications

References 50 publications

Red Cusk-Eel (Genypterus chilensis) Gut Microbiota Description of Wild and Aquaculture Specimens

Red Cusk-Eel (Genypterus chilensis) Gut Microbiota Description of Wild and Aquaculture Specimens

Safety assessment of subchronic feeding of insect-resistant and herbicide-resistant transgenic soybeans to juvenile channel catfish (Ictalurus punctatus)

Construction and Evaluation of Recombinant Lactococcus lactis Expressing the vscO Gene as a Safe Live Oral Vaccine against Vibrio alginolyticus

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