The probiotic mixture IRT5 ameliorates age-dependent colitis in rats

Jeong, Jin-Ju; Woo, Jong Shin; Ahn, Young-Tae; Shim, Jae-Hun; Huh, Chul‐Sung; Im, Sungbin; Han, Myung Joo; Kim, Dong-Hyun

doi:10.1016/j.intimp.2015.04.021

Cited by 25 publications

(20 citation statements)

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“…As far as us aware of, this is the first report describing probiotics can be effective in attenuating autoimmune uveitis or autoimmune dry eye. The immune modulatory effect on ocular autoimmune disease well corresponds with the experimental outcome with IRT-5probiotics in the other autoimmune disease, such as MG, colitis, and EAE of previous reports [ 13 , 14 , 15 ]. The present data indicate that autoreactive T cells in EAU and dry eye may be cross-reactive to the gut antigen, while alloreactive T cells in corneal transplantation are not cross-reactive.…”

Section: Discussionsupporting

confidence: 83%

“…Probiotic IRT-5 (consisting of L. casei , L. acidophilus , L. reuteri , B. bifidum , and S. thermophilus ) powder contains 2 × 10 8 colony forming units (CFU)/g of each strain [ 13 , 14 ]. The total amounts of IRT-5 probiotics were 1 × 10 9 CFU in 300 μL PBS per mice for an oral gavage feeding [ 13 , 14 ]. All the probiotic strains were kindly provided by Korea Yakult Co. (Giheung, Korea).…”

Section: Methodsmentioning

confidence: 99%

“…Recent studies have shown the Lactobacilli and Bifidobacterium -specific beneficial effect in modifying inflammation in rheumatoid arthritis (RA), inflammatory bowel disease (IBD) and atopic dermatitis (AD) [ 10 , 11 , 12 ]. IRT-5 probiotic formulation has immune-modulatory effects in a model of experimental autoimmune myasthenia gravis (MG), colitis, and experimental autoimmune encephalomyelitis (EAE) models, by modifying T cell responses or Treg-Th17 homeostasis [ 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Clinical Effect of IRT-5 Probiotics on Immune Modulation of Autoimmunity or Alloimmunity in the Eye

et al. 2017

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Background: Although the relation of the gut microbiota to a development of autoimmune and inflammatory diseases has been investigated in various animal models, there are limited studies that evaluate the effect of probiotics in the autoimmune eye disease. Therefore, we aimed to investigate the effect of IRT-5 probiotics consisting of Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus reuteri, Bifidobacterium bifidum, and Streptococcus thermophilus on the autoimmunity of uveitis and dry eye and alloimmunity of corneal transplantation. Methods: Experimental autoimmune uveitis was induced by subcutaneous immunization with interphotoreceptor-binding protein and intraperitoneal injection of pertussis toxin in C57BL/6 (B6) mice. For an autoimmune dry eye model, 12-weeks-old NOD.B10.H2b mice were used. Donor cornea of B6 mice was transplanted into BALB/C mice. IRT-5 probiotics or phosphate buffered saline (PBS) were administered for three weeks immediately after induction of uveitis or transplantation. The inflammation score of the retinal tissues, dry eye manifestations (corneal staining and tear secretion), and graft survival were measured in each model. The changes of T cells were evaluated in drainage lymph nodes using fluorescence-activated cell sorting. Results: Retinal histology score in IRT-5 group of uveitis was lower than that in PBS group (p = 0.045). Ocular staining score was lower (p < 0.0001) and tear secretion was higher (p < 0.0001) in the IRT-5 group of NOD.B10.H2b mice than that in the PBS group. However, the graft survival in the IRT-5 group was not different from those of PBS group. The percentage of regulatory T cells was increased in the IRT-5-treated dry eye models (p = 0.032). The percentage of CD8+IL-17hi (p = 0.027) and CD8+ interferon gamma (IFNγ)hi cells (p = 0.022) were significantly decreased in the IRT-5-treated uveitis models and the percentage of CD8+IFNγhi cells was markedly reduced (p = 0.036) in IRT-5-treated dry eye model. Conclusion: Our results suggest that administration of IRT-5 probiotics may modulate clinical manifestations of autoimmunity in the eye, but not on alloimmunity of corneal transplantation.

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Section: Discussionsupporting

confidence: 83%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Clinical Effect of IRT-5 Probiotics on Immune Modulation of Autoimmunity or Alloimmunity in the Eye

et al. 2017

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“…22 IRT5 exerts an anti-inflammatory effect in experimental autoimmune models of myasthenia gravis, colitis, and encephalomyelitis. [22][23][24] Previously, we demonstrated the beneficial effect of IRT5 on the clinical manifestations of autoimmune uveitis and dry eye models but not on those of a corneal allotransplantation model. 25 Although IRT5, especially B. bifidum, has been studied for its anti-inflammatory effects, the precise mechanism by which IRT5 improves dry eye is not known.…”

mentioning

confidence: 99%

IRT5 Probiotics Changes Immune Modulatory Protein Expression in the Extraorbital Lacrimal Glands of an Autoimmune Dry Eye Mouse Model

Choi

Ryu

et al. 2020

Invest. Ophthalmol. Vis. Sci.

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Purpose While the association between the gut microbiome and the immune system has been studied in autoimmune disorders, little is known about ocular disease. Previously we reported that IRT5, a mixture of five probiotic strains, could suppress autoimmune dry eye. In this study, we investigated the mechanism by which IRT5 performs its immunomodulatory function in a mouse model of autoimmune dry eye. Methods NOD.B10.H2b mice were used as an autoimmune dry eye model. Either IRT5 or PBS was gavaged orally for 3 weeks, with or without 5 days of antibiotic pretreatment. The effects on clinical features, extraorbital lacrimal gland and spleen proteins, and fecal microbiota were analyzed. Results The ocular staining score was lower, and tear secretion was higher, in the IRT5-treated groups than in the PBS-treated groups. After IRT5 treatment, the downregulated lacrimal gland proteins were enriched in the biological processes of defense response and immune system process. The relative abundances of 33 operational taxonomic units were higher, and 53 were lower, in the feces of the IRT5-treated groups than in those of the PBS-treated groups. IRT5 administration without antibiotic pretreatment also showed immunomodulatory functions with increases in the Lactobacillus helveticus group and Lactobacillus hamsteri . Additional proteomic assays revealed a decrease of proteins related to antigen-presenting processes in the CD11b + and CD11c + cells of spleen in the IRT5-treated groups. Conclusions Changes in the gut microbiome after IRT5 treatment improved clinical manifestations in the autoimmune dry eye model via the downregulation of antigen-presenting processes in immune networks.

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“…Even though NO is locally produced, its effects can diffuse systemically, thus influencing the progression of the disease [34][35][36] [ [34][35][36]. Bioactive nutrients possibly modulate individual microbiome responses limiting inflammation and free radical synthesis, including NO [37]. Although further studies are necessary to clarify the consequences of pathological NO signaling in different tissues, the study of NO/iNOS-targeted therapeutic strategies might have a clinical benefit [38].…”

Section: Resultsmentioning

confidence: 99%

Reducing the Search Space in Literature-Based Discovery by Exploring Outlier Documents: a Case Study in Finding Links Between Gut Microbiome and Alzheimer’s Disease

Cestnik

Fabbretti

Gubiani

et al. 2017

Genomics Comput Biol

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Literature-based discovery tools have been often used to overcome the problem of fragmentation of science and to assist researchers in their process of cross-domain knowledge discovery.In this paper we propose a methodology for cross-domain literature-based discovery that focuses on outlier documents to reduce the search space of potential cross-domain links and to improve search efficiency. In a previous study, literature mining tools OntoGen for document clustering and CrossBee for cross-domain bridging term exploration were combined to search for hidden relations in scientific papers from two different domains of interest, where the utility of the approach was demonstrated in a study involving PubMed papers about Alzheimer's disease and gut microbiome. This paper extends the approach by proposing a methodology, implemented as a repeatable workflow in a web-based text mining platform TextFlows, which enables easy access and execution of the methodology for the interested researcher.

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The probiotic mixture IRT5 ameliorates age-dependent colitis in rats

Cited by 25 publications

References 35 publications

Clinical Effect of IRT-5 Probiotics on Immune Modulation of Autoimmunity or Alloimmunity in the Eye

Clinical Effect of IRT-5 Probiotics on Immune Modulation of Autoimmunity or Alloimmunity in the Eye

IRT5 Probiotics Changes Immune Modulatory Protein Expression in the Extraorbital Lacrimal Glands of an Autoimmune Dry Eye Mouse Model

Reducing the Search Space in Literature-Based Discovery by Exploring Outlier Documents: a Case Study in Finding Links Between Gut Microbiome and Alzheimer’s Disease

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