The Pro12Ala polymorphism in the PPAR-γ2 gene is not associated to obesity and type 2 diabetes mellitus in a Cameroonian population

Abstract

BackgroundPeroxisome proliferator-activated receptor gamma 2 (PPAR-γ2) is a transcription factor with a key role in adipocyte differentiation, lipid storage and glucose homeostasis. The Ala allele of the common Pro12Ala polymorphism in the isoform PPAR-γ2 is at the center of many controversies because in some populations, it has been observed to be associated with T2DM or obesity but, not in others. The aim of this study was to investigate the association of Pro12Ala polymorphism in the PPAR-γ2 gene with susceptibility to obesity or T2DM in a Cameroonian population.MethodsThis case-control study included 62 obese, 60 T2DM patients and 120 controls (60 non obese and 60 patients without T2DM), all unrelated and of Cameroonian origin. PPAR-γ2 was examined by genotyping for Pro12Ala using the Restriction Fragment Length Polymorphism - Polymerase Chain Reaction (PCR - RFLP).ResultsA portion of the 270 base pair bands of the PPAR-γ2 gene was successfully amplified. The Ala12 variant was totally absent from the study population, all participants being homozygote Pro/Pro.ConclusionPPAR-γ2 Pro12Ala gene polymorphism may not be associated with obesity and T2DM. These results suggest that, PPAR-γ2 is unlikely a major gene for obesity or T2DM in the study population.

Peroxisome proliferator-activated receptor-γ polymorphism (rs1801282) is associated with obesity in Egyptian patients with coronary artery disease and type 2 diabetes mellitus

“…The relation between PPAR-γ Pro12Ala polymorphism and obesity in recent studies is still conflicting, some researchers linked PPAR-γ Pro12Ala polymorphism with waist circumference in patients having T2DM [8], while others did not find an association between PPAR-γ Pro12Ala polymorphism and obesity [34], [35], [36].…”

Association between LEPR, FTO, MC4R, and PPARG-2 polymorphisms with obesity traits and metabolic phenotypes in school-aged children

“…PPARG isoforms 1 and 2 are transcription factors that activate adipocyte differentiation and mediate the expression of specific fat cell genes [ 28 ]. However, PPARG-2 rs1801282 may not be associated with obesity and type 2 diabetes mellitus [ 50 ]. Although we did not detect significant associations, the results showed a trend toward reduced mean BMI in heterozygous overweight/obese children than in homozygous wild type.…”

The role of TGF-β1 869 T > C and PPAR γ2 34 C > G polymorphisms, fat mass, and anthropometric characteristics in predicting childhood obesity at birth

“…Other studies underlined that thin patients’ carriers of the variant gene have a diminished expression of this gene in their visceral tissue. [41] Mato et al [56] did not obtain any correlation between the polymorphism of this gene and obesity, and type 2 diabetes mellitus, respectively.…”