The primary structure of rat ribosomal protein S16

Chan, Yuen‐Ling; Paz, Veronica; Olvera, Joe; Wool, Ira G.

doi:10.1016/0014-5793(90)80711-q

Cited by 76 publications

(36 citation statements)

References 15 publications

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“…Four hundred nanograms of total RNA was reverse transcribed with avian myeloblastosis virus reverse transcriptase, random primers, and deoxy-NTPs at 37°C for 75 min, and heating at 95°C for 5 min terminated the reaction. Target cDNA amplification was performed by PCR and using published sequences (14)(15)(16)(17)(18)22) conducted four to five times for each gene of interest. Densitometric signals from individual bands were divided by the respective density for rat ribosomal protein S16 to correct for differences in gel loading (Kodak).…”

Section: Analysis Of Serum Mehp and Dehp Levels By Hplcmentioning

confidence: 99%

Phthalate-induced Leydig cell hyperplasia is associated with multiple endocrine disturbances

Akingbemi

Klinefelter

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

317

198

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The possibility that exposures to environmental agents are associated with reproductive disorders in human populations has generated much public interest recently. Phthalate esters are used most commonly as plasticizers in the food and construction industry, and di-(2-ethylhexyl) phthalate (DEHP) is the most abundant phthalate in the environment. Daily human exposure to DEHP in the U.S. is significant, and occupational and clinical exposures from DEHP-plasticized medical devices, e.g., blood bags, hemodialysis tubing, and nasogastric feeding tubes, increase body burden levels. We investigated the effects of chronic exposures to low environmentally relevant DEHP levels on testicular function. Our data show that prolonged exposures to this agent induced high levels of the gonadotropin luteinizing hormone and increased the serum concentrations of sex hormones [testosterone and 17␤-estradiol (E2)] by >50%. Increased proliferative activity in Leydig cells was evidenced by enhanced expression of cell cycle proteins, as determined by RT-PCR. The numbers of Leydig cells in the testis of DEHP-treated rats were 40 -60% higher than in control rats, indicating induction of Leydig cell hyperplasia. DEHP-induced elevations in serum testosterone and E2 levels suggest the possibility of multiple crosstalks between androgen, estrogen, and steroid hormone receptors, whereas the presence of estrogen receptors in nonreproductive tissues, e.g., cardiovascular system and bones, implies that the increases in serum E2 levels have implications beyond reproduction, including systemic physiology. Analysis of the effects of phthalate exposures on gonadotropin and steroid hormone levels should form part of overall risk assessment in human populations. R eports of a higher incidence of urogenital anomalies of the newborn, such as cryptorchidism, hypospadias, and reproductive abnormalities in wild life exposed to high levels of chemicals in the environment, have generated public concern that these agents may impair human reproductive health (1, 2). Phthalates are used as plasticizers in certain infant toys and consumer products (e.g., containers for soaps, shampoos, and perfumes) and medical devices such as tubings and catheters. The U.S. Department of Health and Human Services in 1985 (3) estimated the total daily human consumption of di-(2-ethylhexyl) phthalate (DEHP) from all sources of exposure at 5.8 mg in the U.S. In a report just published by the Center for Disease Control and Prevention, the urinary levels of mono-(ethylhexyl) phthalate (MEHP) (micrograms per liter), which is the chief metabolite of DEHP, ranged from 3.26 to 4.15 in males and 2.93 to 3.51 in females; these levels are thought to represent only one-tenth of the ingested DEHP dose within the previous 24 h (4). In a recent review of laboratory studies, the U.S. National Toxicology Program's Center for the Evaluation of Risks to Human Reproduction Expert Panel concluded that DEHP has the potential to produce adverse reproductive effects in humans (5). Indeed, several propo...

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Section: Analysis Of Serum Mehp and Dehp Levels By Hplcmentioning

confidence: 99%

Phthalate-induced Leydig cell hyperplasia is associated with multiple endocrine disturbances

Akingbemi

Klinefelter

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

317

198

View full text Add to dashboard Cite

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“…The cDNA insert in pL26-5 was made radioactive and used to probe digests made with the restriction endonucleases BumHI, EcoRI, and Hind111 from rat liver nuclear DNA [5]. The number of hybridization bands suggest that there are 8-16 copies of the L26 gene ( fig.2A).…”

Section: Resultsmentioning

confidence: 99%

“…Radioactive rat ribosomal protein L26 cDNA was hybridized to genomic DNA that had been digested with restriction enzymes [5], and to a preparation of rat liver poly(A)+ mRNA [6].…”

Section: Methodsmentioning

confidence: 99%

The primary structure of rat ribosomal protein L26

et al. 1989

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The amino acid sequence of rat ribosomal protein L26 was deduced from the sequence of nucleotides in a recombinant cDNA and confirmed from the NH,-terminal amino acid sequence of the protein. Rat L26 contains 145 amino acids and has a molecular mass of 17 266 Da. Hybridization of the cDNA to digests of nuclear DNA suggests that there are 8-16 copies of the L26 gene. The mRNA for the protein is about 650 nucleotides in length. Protein L26 has a sequence of 9 residues that may be repeated in three places.Ribosomal protein L26; Amino acid sequence; cDNA; Elongation factor-2 binding domain; (Rat)

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“…The cDNA insert in pL34-1 was made radioactive and used to probe digests made with the restriction endonucleases BarnHI, EcoRI, and Hind111 from rat liver nuclear DNA [3]. The number of hybridization bands suggest that there are 7-9 copies of the L34 gene ( fig.%A).…”

Section: Resultsmentioning

confidence: 99%

“…Radioactive rat ribosomal protein L34 cDNA was hybridized to restriction enzyme digests of genomic DNA [3], and to a preparation of rat liver poly(A)+ mRNA [4].…”

Section: Methodsmentioning

confidence: 99%

The primary structure of rat ribosomal protein L34

1989

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The amino acid sequence of rat ribosomal protein L34 was deduced from the nucleotide sequence in a recombinant cDNA and confirmed from the NH,-terminal amino acid sequence of the protein. Ribosomal protein L34 contains 117 amino acids (the NH,-terminal methionine is removed after translation of the mRNA) and has an h4, of 13498. Hybridization of the cDNA to digests of nuclear DNA suggests that there are 7-9 copies of the L34 gene. The mRNA for the protein is about 630 nucleotides in length.Ribosomal protein L34; Amino acid sequence; cDNA; (Rat)

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The primary structure of rat ribosomal protein S16

Cited by 76 publications

References 15 publications

Phthalate-induced Leydig cell hyperplasia is associated with multiple endocrine disturbances

Phthalate-induced Leydig cell hyperplasia is associated with multiple endocrine disturbances

The primary structure of rat ribosomal protein L26

The primary structure of rat ribosomal protein L34

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