1975

DOI: 10.1016/0014-5793(75)80703-4

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The primary structure of a non‐initiating methionine specific tRNA from brewer's yeast

Hartmut Gruhl¹,

Horst Feldmann²

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1976

1976

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1978

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Cited by 12 publications

(1 citation statement)

References 19 publications

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“…It remains to be seen which other structural features could contribute to the specificity in this reaction. Some of these considerations have already been presented in a short communication [21].…”

Section: Pmentioning

confidence: 99%

The Primary Structure of a Non-initiating Methionine-Specific tRNA from Brewer's Yeast

1976

Eur J Biochem

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tRNA3Met, one of the non‐initiating methionine‐specific tRNAs in brewer's yeast was purified from bulk tRNA labelled with [32P]phosphate by two column chromatographic steps. The primary structure of this tRNA was determined by the usual fingerprinting technique. Analyses of the isolated nucleotides and oligonucleotides from digests with pancreatic and T1 ribonucleases were in good agreement and stated that tRNA3Met consists of 76 nucleotide residues including 13 minor nucleotides. Overlaps from which the complete sequence could be deduced were derived from the analyses of 15 fragments obtained by partial digestion with T1 ribonuclease.

“…It remains to be seen which other structural features could contribute to the specificity in this reaction. Some of these considerations have already been presented in a short communication [21].…”

Section: Pmentioning

confidence: 99%

The Primary Structure of a Non-initiating Methionine-Specific tRNA from Brewer's Yeast

1976

Eur J Biochem

View full text Add to dashboard Cite

tRNA3Met, one of the non‐initiating methionine‐specific tRNAs in brewer's yeast was purified from bulk tRNA labelled with [32P]phosphate by two column chromatographic steps. The primary structure of this tRNA was determined by the usual fingerprinting technique. Analyses of the isolated nucleotides and oligonucleotides from digests with pancreatic and T1 ribonucleases were in good agreement and stated that tRNA3Met consists of 76 nucleotide residues including 13 minor nucleotides. Overlaps from which the complete sequence could be deduced were derived from the analyses of 15 fragments obtained by partial digestion with T1 ribonuclease.

Translation of Messenger RNA

1977

The Ribonucleic Acids

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No abstract

The Transfer Ribonucleic Acids

¹

1978

The Genetic Mechanism and the Origin of Life

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No abstract

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