2011
DOI: 10.1111/j.1751-1097.2011.00903.x
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The Primary Photophysics of the Avena sativa Phototropin 1 LOV2 Domain Observed with Time‐resolved Emission Spectroscopy

Abstract: The phototropins are blue-light receptors that base their light-dependent action on the reversible formation of a covalent bond between a flavin mononucleotide (FMN) cofactor and a conserved cysteine in light, oxygen or voltage (LOV) domains. The primary reactions of the Avena sativa phototropin 1 LOV2 domain were investigated by means of time-resolved and low-temperature fluorescence spectroscopy. Synchroscan streak camera experiments revealed a fluorescence lifetime of 2.2 ns in LOV2. A weak long-lived compo… Show more

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Cited by 18 publications
(22 citation statements)
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“…Time-resolved fluorescence measurements were carried out with a streak camera setup, as described previously373839 at room temperature (~20 °C). Femtosecond laser pulses from an integrated Ti:Sapphire oscillator (Vitesse, Coherent, 800 nm, 80 MHz, 100 fs) was incorporated into a Ti:Sapphire regenerative amplifier (RegA, Coherent, 800 nm, 50 kHz, 100 fs).…”
Section: Methodsmentioning
confidence: 99%
“…Time-resolved fluorescence measurements were carried out with a streak camera setup, as described previously373839 at room temperature (~20 °C). Femtosecond laser pulses from an integrated Ti:Sapphire oscillator (Vitesse, Coherent, 800 nm, 80 MHz, 100 fs) was incorporated into a Ti:Sapphire regenerative amplifier (RegA, Coherent, 800 nm, 50 kHz, 100 fs).…”
Section: Methodsmentioning
confidence: 99%
“…In crystal structures of LOV proteins, typically two orientations of the active site Cys residue are observed with only the conformation placing the cysteine thiol moiety above the C4a position competent for photoactivation [55,57]. Following excitation by blue-light, the flavin is promoted to an excited singlet state that rapidly converts to a triplet species [53,54,58,59]. The flavin triplet then causes an electron transfer event between the flavin and active site Cys to generate a flavin-semiquinone and a Cys-radical [37,58].…”
Section: Lov Domain Photocyclementioning
confidence: 99%
“…Thel ight-adapted signaling state of light-activated LOVp hotoreceptors is generated through the formation of af lavin-(C4a)-cysteinyl covalent bond with ac onserved cysteine residue in the active site of LOVd omains (Figure 1). [5,6] In the past decade,t he excited-state dynamics of the dark-adapted state of LOVp hotoreceptors has been thoroughly investigated experimentally [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24] while there have been less such studies on the light-adapted state.…”
mentioning
confidence: 99%