The preventive effects of neural stem cells and mesenchymal stem cells intra-ventricular injection on brain stroke in rats

Abstract

Introduction:Stroke is one of the most important causes of disability in developed countries and, unfortunately, there is no effective treatment for this major problem of central nervous system (CNS); cell therapy may be helpful to recover this disease. In some conditions such as cardiac surgeries and neurosurgeries, there are some possibilities of happening brain stroke. Inflammation of CNS plays an important role in stroke pathogenesis, in addition, apoptosis and neural death could be the other reasons of poor neurological out come after stroke. In this study, we examined the preventive effects of the neural stem cells (NSCs) and mesenchymal stem cells (MSCs) intra-ventricular injected on stroke in rats.Aim:The aim of this study was to investigate the preventive effects of neural and MSCs for stroke in rats.Materials and Methods:The MSCs were isolated by flashing the femurs and tibias of the male rats with appropriate media. The NSCs were isolated from rat embryo ganglion eminence and they cultured NSCs media till the neurospheres formed. Both NSCs and MSCs were labeled with PKH26-GL. One day before stroke, the cells were injected into lateral ventricle stereotactically.Results:During following for 28 days, the neurological scores indicated that there are better recoveries in the groups received stem cells and they had less lesion volume in their brain measured by hematoxylin and eosin staining. Furthermore, the activities of caspase-3 were lower in the stem cell received groups than control group and the florescent microscopy images showed that the stem cells migrated to various zones of the brains.Conclusion:Both NSCs and MSCs are capable of protecting the CNS against ischemia and they may be good ways to prevent brain stroke consequences situations.

Neuroprotective Effects of Sulphated Agaran from Marine Alga Gracilaria cornea in Rat 6‐Hydroxydopamine Parkinson's Disease Model: Behavioural, Neurochemical and Transcriptional Alterations

“…Many existing pharmaceuticals are rendered ineffective in the treatment of cerebral diseases due to our inability to effectively deliver and sustain them within the brain . Thus, the intracranial administration of drugs, natural bioactives and cells is utilized by some studies as a primary option for analysis of local effects in specific areas of interest on the brain .…”

Niche astrocytes promote the survival, proliferation and neuronal differentiation of co-transplanted neural stem cells following ischemic stroke in rats

“…While conventional therapeutic strategies focus on preventing brain damage, stem cell transplantation has the potential to repair the injured brain tissue. Recently, neural stem cells (NSCs) have been developed and used in preclinical studies as therapy for stroke (3,4).…”

“…NSCs reside in specialized microenvironments, or 'niches', that regulate their self-renewal and differentiation activities (13). For example, the continuous production of neuroblasts is maintained by the SVZ microenvironmental niche (3,4). It is widely believed that enhancing the neurogenic activities of endogenous NSCs will provide a much-needed therapeutic option for brain injuries (14).…”

Optimization of time for neural stem cells transplantation for brain stroke in rats

“…Noticeable amongst these are neural stem cells (3,4), bone marrow stem cells (3,5,6), cord blood derived MSCs (7,8), adipose tissue derived (5), embryonic stem cells and their derivatives (9), and induced pluripotent stem cells (iPSCs) (10,11). Nevertheless, ease of availability without moral and ethical strings and differentiation capacity to adopt desired phenotype are vital issues for the search of ideal donor cells for transplantation therapy.…”