The preventive effect of taxifolin on acrylamide-induced heart damage in rats

Abstract

Objective Acrylamide is a toxic compound widely used in industrial sectors. Acrylamide causes reactive oxygen species formation and the subsequent lipid peroxidation reaction, which plays an important role in the pathogenesis of oxidative damage. Taxifolin is a flavonoid with antioxidant properties that inhibit reactive oxygen species formation. In this study, we aimed to investigate the preventive effect of taxifolin on acrylamide-induced oxidative heart damage. Methods The rats were divided into three groups: Acrylamide, Acrylamide+Taxifolin , and Healthy group. Water and food intake and body weight alterations were recorded daily. Malondialdehyde, total glutathione, nuclear factor kappa-B, total oxidant status, and total antioxidant status levels were analyzed from the heart tissue. Troponin-I levels, the parameter known as a cardiac biomarker, were analyzed from the blood sample. The cardiac histopathologic examination was also performed. Results In the Acrylamide group animals, the malondialdehyde, nuclear factor kappa-B, total oxidant status, and troponin-I levels were significantly higher compared to the ones of Acrylamide+Taxifolin and Healthy groups. The levels of total glutathione and total antioxidant status were significantly lower compared to Acrylamide+Taxifolin and Healthy groups’. Additionally, in the Acrylamide group, body weight gain, food and water intake, significantly declined compared to the Acrylamide+Taxifolin and Healthy groups. However, in the Acrylamide+Taxifolin group, taxifolin supplementation brought these values close to Healthy group ones. Furthermore, taxifolin treatment ameliorated structural myocardial damage signs induced by acrylamide. Conclusion Acrylamide exposure significantly induced oxidative damage to rat heart tissue. Taxifolin was able to improve the toxic consequences of acrylamide biochemically and histopathologically, possibly due to its antioxidant properties.

Cardioprotective Effect of Taxifolin against Isoproterenol-Induced Cardiac Injury through Decreasing Oxidative Stress, Inflammation, and Cell Death, and Activating Nrf2/HO-1 in Mice

“…In accordance with our results, several investigations have shown that the cardioprotective action of TAX is associated with reduced oxidative markers and elevated antioxidant components, resulting in ameliorated oxidative injury to the myocardium [ 39 , 40 , 49 ]. Furthermore, TAX exhibited tissue protective effect against chemically-induced oxidative damage in various experimental models, such as acrylamide-induced heart [ 21 ] and kidney injury [ 50 ], alcohol [ 25 ] and carbon tetrachloride (CCl 4 )-induced liver injury [ 25 ], and methanol-induced oxidative optic nerve damage [ 51 ].…”

“…(+)-Taxifolin (TAX; C 15 H 12 O 7 , Figure 1 ) is a natural flavonoid present mainly in olive oil, grapes, and onions, and one which possesses several pharmacological actions, including radical scavenging and anti-inflammatory properties [ 19 , 20 ]. Indeed, TAX was shown to prevent against acrylamide-induced heart damage in rats by decreasing oxidative stress and enhancing antioxidants [ 21 ]. Furthermore, TAX protected against renal damage induced by unilateral ureteral obstruction (UUO) in rats via suppressing oxidative tissue injury, inflammation, and fibrosis [ 22 ].…”