The Preventive Effect of Curcumin on the Experimental Rat Epidural Fibrosis Model

Demirel, Cem; Turkoz, Dursun; Yazıcıoğlu, İrem Melike; Çokluk, Cengiz

doi:10.1016/j.wneu.2020.09.140

Cited by 5 publications

(1 citation statement)

References 49 publications

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“…We found that the scaffolds containing CURC induced a lower level of fibrosis between the scaffold and the spinal cord tissue, as evaluated by a macroscopic analysis of the H&E staining (data not shown). A recent study developed in a laminectomy model in which curcumin prevented peridural fibrotic tissue formation supports this finding [ 47 ]. The anti-fibrotic properties could be attributed to the capability of curcumin to suppress collagen production through down-regulation of transforming growth factor-beta 1 [ 48 , 49 ].…”

Section: Discussionmentioning

confidence: 57%

A Hyaluronic Acid Demilune Scaffold and Polypyrrole-Coated Fibers Carrying Embedded Human Neural Precursor Cells and Curcumin for Surface Capping of Spinal Cord Injuries

et al. 2021

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Tissue engineering, including cell transplantation and the application of biomaterials and bioactive molecules, represents a promising approach for regeneration following spinal cord injury (SCI). We designed a combinatorial tissue-engineered approach for the minimally invasive treatment of SCI—a hyaluronic acid (HA)-based scaffold containing polypyrrole-coated fibers (PPY) combined with the RAD16-I self-assembling peptide hydrogel (Corning® PuraMatrix™ peptide hydrogel (PM)), human induced neural progenitor cells (iNPCs), and a nanoconjugated form of curcumin (CURC). In vitro cultures demonstrated that PM preserves iNPC viability and the addition of CURC reduces apoptosis and enhances the outgrowth of Nestin-positive neurites from iNPCs, compared to non-embedded iNPCs. The treatment of spinal cord organotypic cultures also demonstrated that CURC enhances cell migration and prompts a neuron-like morphology of embedded iNPCs implanted over the tissue slices. Following sub-acute SCI by traumatic contusion in rats, the implantation of PM-embedded iNPCs and CURC with PPY fibers supported a significant increase in neuro-preservation (as measured by greater βIII-tubulin staining of neuronal fibers) and decrease in the injured area (as measured by the lack of GFAP staining). This combination therapy also restricted platelet-derived growth factor expression, indicating a reduction in fibrotic pericyte invasion. Overall, these findings support PM-embedded iNPCs with CURC placed within an HA demilune scaffold containing PPY fibers as a minimally invasive combination-based alternative to cell transplantation alone.

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Section: Discussionmentioning

confidence: 57%