The prevalence, putative virulence genes and antibiotic resistance profiles of Aeromonas spp. isolated from cultured freshwater fishes in peninsular Malaysia

Azzam-Sayuti, Mohamad; Ina‐Salwany, Yasin; Zamri‐Saad, Mohd; Yusof, Mohd Termizi; Salleh, Annas; Najihah, Yunus; Liles, Mark R.; Monir, Shirajum; Zaidi, Zairy; Amal, Mohammad Noor Azmai

doi:10.1016/j.aquaculture.2021.736719

Cited by 33 publications

(31 citation statements)

References 42 publications

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“…It is worth mentioning that no abnormalities were observed following infection by the myoinositol utilizing A. dhakensis 1P11S3, indicating that inositol catabolism per se did not contribute to A. dhakensis pathogenesis in tilapia with current disease model. Furthermore, all isolates used in this study were obtained from diseased fish, without any relation with epidemic outbreak [32]. On the other hand, myo-inositol utilizing A. hydrophila vAh, that were isolated during epidemic outbreak among catfish, have been reported to cause severe clinical MAS symptoms with rapid onset of mortality [27].…”

Section: Discussionmentioning

confidence: 99%

“…When the LD 50 of each isolate was compared with the presence of virulence genes [32], A. dhakensis 4PS2 and A. hydrophila 8TK3 were found to have all of the virulence genes that were tested. In addition, both strains also possessed the aer gene, which might play a significant role in the virulence of Aeromonas.…”

Section: Discussionmentioning

confidence: 99%

“…A total of 124 Aeromonas isolates that were previously obtained from fish with chronic ulcerative infection were used. The isolates were identified in previous experiment [32] as A. dhakensis (n = 53), A. hydrophila (n = 25), A. veronii (n = 27), A. caviae (n = 10), and A. jandaei (n = 9) using primers that identified the gyrB gene [33]. The isolates were streaked onto tryptic soy agar (TSA) (Oxoid, Hampshire, UK) and further sub-cultured onto Aeromonas selective agar (ASA) (Oxoid, Hampshire, UK) and incubated at 30 • C overnight.…”

Section: Collection Of Aeromonas Sppmentioning

confidence: 99%

“…Six isolates of Aeromonas spp. were selected for the pathogenicity study based on the number of virulence genes present in each isolate [32]. The selected isolates were the myo-inositol utilizing A. dhakensis 1P11S3 and the non-myo-inositol utilizing A. dhakensis 4PS2, A. hydrophila 8TK3, A. veronii 6TS5, A. caviae 7X11, and A. jandaei 7KL3.…”

Section: Preparation Of Different Concentrations Of Aeromonas Sppmentioning

confidence: 99%

“…The five non-myo-inositol utilizing Aeromonas spp. had highest number of virulence genes within each species (Table A1) [32]. Briefly, five colonies on ASA were randomly picked and inoculated into 1 L of tryptic soy broth (TSB; Oxoid), and incubated for 18 h at 30 • C. Serial dilutions were performed up to tenfold and 1 mL of TSB from each dilution was plated on TSA in triplicate, and further incubated at 30 • C overnight.…”

Section: Preparation Of Different Concentrations Of Aeromonas Sppmentioning

confidence: 99%

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Comparative Pathogenicity of Aeromonas spp. in Cultured Red Hybrid Tilapia (Oreochromis niloticus × O. mossambicus)

Azzam-Sayuti

Ina‐Salwany

Zamri‐Saad

et al. 2021

Biology

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The genus Aeromonas has been recognised as an important pathogenic species in aquaculture that causes motile Aeromonas septicaemia (MAS) or less severe, chronic infections. This study compares the pathogenicity of the different Aeromonas spp. that were previously isolated from freshwater fish with signs of MAS. A total of 124 isolates of Aeromonas spp. were initially screened for the ability to grow on M9 agar with myo-inositol as a sole carbon source, which is a discriminatory phenotype for the hypervirulent A. hydrophila (vAh) pathotype. Subsequently, LD50 of six selected Aeromonas spp. were determined by intraperitoneal injection of bacterial suspension containing 103, 105, and 107 CFU/mL of the respective Aeromonas sp. to red hybrid tilapias. The kidneys, livers and spleens of infected moribund fish were examined for histopathological changes. The screening revealed that only A. dhakensis 1P11S3 was able to grow using myo-inositol as a sole carbon source, and no vAh strains were identified. The LD50–240h of A. dhakensis 1P11S3 was 107 CFU/mL, while the non-myo-inositol utilizing A. dhakensis 4PS2 and A. hydrophila 8TK3 was lower at 105 CFU/mL. Similarly, tilapia challenged with the myo-inositol A. dhakensis 1P11S3 showed significantly (p < 0.05) less severe signs, gross and histopathological lesions, and a lower mortality rate than the non-myo-inositol A. dhakensis 4PS2 and A. hydrophila 8TK3. These findings suggested that myo-inositol utilizing A. dhakensis 1P11S3 was not a hypervirulent Aeromonas sp. under current experimental disease challenge conditions, and that diverse Aeromonas spp. are of concern in aquaculture farmed freshwater fish. Therefore, future study is warranted on genomic level to further elucidate the influence of myo-inositol utilizing ability on the pathogenesis of Aeromonas spp., since this ability correlates with hypervirulence in A. hydrophila strains.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Collection Of Aeromonas Sppmentioning

confidence: 99%

Section: Preparation Of Different Concentrations Of Aeromonas Sppmentioning

confidence: 99%

Section: Preparation Of Different Concentrations Of Aeromonas Sppmentioning

confidence: 99%

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Comparative Pathogenicity of Aeromonas spp. in Cultured Red Hybrid Tilapia (Oreochromis niloticus × O. mossambicus)

Azzam-Sayuti

Ina‐Salwany

Zamri‐Saad

et al. 2021

Biology

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Streptomyces enissocaesilis L-82 has broad-spectrum antibacterial activity and promotes growth for Carassius auratus

Long,

Zhao,

et al. 2024

Appl Microbiol Biotechnol

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Aeromonas is the main pathogen causing bacterial diseases in fish. The disadvantages of chemical drugs to control fish diseases have been highlighted, and it is urgent to find an eco-friendly control method. In this study, an actinomycete strain with antibacterial activity against fish pathogenic bacteria was screened from soil samples. Combined with morphological characteristics, physiological and biochemical characteristics, and gyrB gene and whole genome comparison analysis, it was identified as a new strain of Streptomyces enissocaesilis, named Streptomyces enissocaesilis L-82. The strain has broad-spectrum antibacterial activity against fish pathogens. A substance with a mass-to-charge ratio of 227.20 [M + H] + was isolated and purified by high-performance liquid chromatography and mass spectrometry. It was presumed to be a derivative of 5-dimethylallylindole-3-acetonitrile. The strain is safe and non-toxic to crucian carp, and can stably colonize crucian carp and inhibit the proliferation of A. hydrophila. After feeding the feed containing 1 × 108 CFU/mL strain concentration, the weight growth rate and specific growth rate of crucian carp increased, the activity of ACP and SOD in serum increased, and the survival rate of crucian carp increased after challenge. Genome-wide analysis showed that the strain had strong ability to metabolize and tolerate extreme environments. And has a strong potential for disease resistance. Therefore, the strain is expected to be developed as a feed additive for fish farming. Key points • The new Streptomyces enissocaesilis L-82 has a broad spectrum and stable antibacterial activity and meets the safety standards of feed additives. • Strain L-82 can colonize crucian carp, improve the growth, antioxidant, and immune performance of the host, and improve the survival rate after being infected with A. hydrophila. • Genome-wide analysis suggests that the strain has great disease resistance potential and is expected to be developed as a feed additive for fish culture.

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In vivo assessment of Lactobacillus plantarum strains in black tiger shrimp (Penaeus monodon): implications for growth performance, probiotic-pathogen interaction, and defense against AHPND infection

Chin,

Haifa-Haryani,

Nazarudin

et al. 2024

Aquacult Int

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The prevalence, putative virulence genes and antibiotic resistance profiles of Aeromonas spp. isolated from cultured freshwater fishes in peninsular Malaysia

Cited by 33 publications

References 42 publications

Comparative Pathogenicity of Aeromonas spp. in Cultured Red Hybrid Tilapia (Oreochromis niloticus × O. mossambicus)

Comparative Pathogenicity of Aeromonas spp. in Cultured Red Hybrid Tilapia (Oreochromis niloticus × O. mossambicus)

Streptomyces enissocaesilis L-82 has broad-spectrum antibacterial activity and promotes growth for Carassius auratus

In vivo assessment of Lactobacillus plantarum strains in black tiger shrimp (Penaeus monodon): implications for growth performance, probiotic-pathogen interaction, and defense against AHPND infection

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