The prevalence of plasmid-mediated quinolone resistance genes among Escherichia coli strains isolated from urinary tract infections in southwest Iran

Jomehzadeh, Nabi; Saki, Morteza; Ahmadi, Khadijeh; Zandi, Golshan

doi:10.1007/s11033-022-07215-5

Cited by 9 publications

(10 citation statements)

References 32 publications

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“…The Antibiotic Resistance Profile of E. coli is expected as antibiotic resistance is now a common phenomenon that is known to be responsible for 1.27 million global deaths directly in 2019 and contributed to 4.95 million deaths about 10million for deaths [26] . Although, the resistance to ciprofloxacin (being the antibiotic of interest in this research being a quinolone) was low (9.8%) in comparison to other antibiotics used in this study such as the ones mentioned above, it is however still lower when compared to 18.8% as reported by Silva [27].…”

Section: Discussioncontrasting

confidence: 50%

Molecular Characterization of Plasmid-Mediated Quinolone resistance in Urinary Escherichia coli from some Healthcentres in Nasarawa-West Senatorial District, Nasarawa State, Nigeria

Kefas Osayi Eko,

Yakubu Boyi Ngwai,

David Ishaleku

et al. 2024

GSC Adv. Res. Rev.

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The most common bacteria associated with urinary tract infections (UTIs) worldwide Escherichia coli. To treat UTIs, Fluoroquinolones are widely used especially Ciprofloxacin. The honey moon did not last long due to the development of resistance acquired by the bacteria as a result of resistance gene. This study investigated ciprofloxacin resistant urinary E. coli resistance via plasmid-mediated quinolone resistance (PMQR) genes rather than the chromosomal mutation of patients attending some Healthcentres in Nasarawa-West Senatorial District, Nasarawa State, Nigeria. Ninety-Two (92) 42% confirmed ciprofloxacin urinary resistant E. coli from patients attending the three (3) study Centres were screened for the presence of PMQR resistant genes (aac(6’)-Ib-cr, oqxA, oqxB, qnrA, qnrB, qnrS) using polymerase chain reaction (PCR) method. The screening detected the presence of QnrA 4(44.4%), QnrB and QnrS both with 5(55.6%) occurrence, meaning aac(6’)-Ib-cr, QnrVC ,GyrA, ParC and others were not detected. It is clear that these isolates harbored some PMQR genes both singly and in combination. This is a strong warning to both the prescribers and users of Antibiotic of the consequences of negligence of strict adherence to the usage of antimicrobials, since resistance to antibiotics has led to death of great number of People worldwide.

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Section: Discussioncontrasting

confidence: 50%

Molecular Characterization of Plasmid-Mediated Quinolone resistance in Urinary Escherichia coli from some Healthcentres in Nasarawa-West Senatorial District, Nasarawa State, Nigeria

Kefas Osayi Eko,

Yakubu Boyi Ngwai,

David Ishaleku

et al. 2024

GSC Adv. Res. Rev.

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“…Contrary to our findings, Rahimi et al [ 23 ] revealed that the sole E. coli O157 isolate from camel meat in Iran was deficient in eaeA and ehlyA virulent genes. In the same country, the markedly genetic resistance of the human-originating E. coli strains was recently demonstrated in a study conducted by Jomehzadeh et al [ 44 ].…”

Section: Resultsmentioning

confidence: 98%

Cefotaxime-, Ciprofloxacin-, and Extensively Drug-Resistant Escherichia coli O157:H7 and O55:H7 in Camel Meat

Sallam

Abd-Elrazik

Raslan³

et al. 2023

Foods

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The present study aimed to explore for the first time the occurrence and the antimicrobial resistance profiles of E. coli O157:H7 and O55:H7 isolates in camel meat in Egypt. Among the 110 camel meat samples examined using standardized microbiological techniques, 10 (9.1%) and 32 (29.1%) were positive for E. coli O157:H7 and E. coli O55:H7, respectively. In total, 24 isolates were verified as E. coli O157:H7, while 102 isolates were confirmed serologically as E. coli O55:H7. Multiplex PCR revealed the existence of eaeA, stx1, stx2, and EHEC-hlyA among E. coli O157:H7 and O55:H7 isolates (n = 126) at various percentages. According to their resistance against 14 antibiotics, 16.7% and 83.3% of O157:H7 isolates and 8.6% and 76.5% of O55:H7 isolates were classified into extensively drug-resistant and multi-drug-resistant, respectively, whereas 29.4% and 22.2% of E. coli isolates were resistant to cefotaxime and ciprofloxacin, respectively. The study results emphasize that camel meat may be a vehicle for multi- and extensively drug-resistant E. coli O157:H7 and O55:H7 strains, indicating a potential threat to public health. Further studies based on the molecular evidence of the antimicrobial resistance genes and enrolling a larger number of samples are recommended for a better understanding of the antimicrobial resistance phenomenon of camel-meat-originating pathogenic E. coli strains.

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“…The results indicate that ESBL‐EC has higher antibiotic resistance than COL‐EC, even for the same plasmid‐mediated antibiotic‐resistant E. coli . There are also reports of the quinolone gene qnr (Jomehzadeh et al, 2022) and the FOS resistance gene fosA encoded in ESBL plasmids (Xiaoliang et al, 2019). The acquisition of ESBL‐related genes is reportedly involved in the acquisition of quinolone and aminoglycoside resistance (Tayh et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Acquisition of cephalosporin resistance genes bla_CTX‐M_‐55, bla_CTX‐M_‐65, and bla_CMY_‐2 leads to quinolone resistance in colistin‐resistant Escherichia coli harboring mcr‐1 gene

Nakayama,

Li,

Ngo

et al. 2024

Journal of Food Safety

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Food contamination with plasmid‐mediated antibiotic‐resistant Escherichia coli has not been thoroughly investigated. This study aimed to clarify the prevalence of colistin‐resistant AmpC/extended‐spectrum β‐lactamase (ESBL)‐producing E. coli harbouring mcr (COL‐ESBL‐EC) and to determine antibiotic resistance by comparison with AmpC/ESBL‐producing E. coli (ESBL‐EC) and colistin‐resistant E. coli harbouring mcr (COL‐EC). Sixty chicken meats were tested for COL‐ESBL‐EC contamination. The result showed that 64 COL‐ESBL‐EC were isolated from 66.7% of the samples, and compared with ESBL‐EC and COL‐EC were isolated before. The genotypes of the COL‐ESBL‐EC showed blaCTX‐M‐55/TEM, blaCTX‐M‐55, blaCMY‐2, and blaCTX‐M‐65 were predominant. The COL‐ESBL‐EC results showed a similar trend for blaCTX‐M identification to that of ESBL‐EC. The mcr was investigated, and mcr‐1 was detected in COL‐ESBL‐EC and COL‐EC. Antibiotic susceptibility testing revealed that many strains of COL‐ESBL‐EC and ESBL‐EC were resistant to quinolones, and fosfomycin (FOS). These results suggest that COL‐ESBL‐EC has simultaneously acquired AmpC/ESBL‐related, quinolone, and FOS resistance genes in COL‐EC.

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The prevalence of plasmid-mediated quinolone resistance genes among Escherichia coli strains isolated from urinary tract infections in southwest Iran

Cited by 9 publications

References 32 publications

Molecular Characterization of Plasmid-Mediated Quinolone resistance in Urinary Escherichia coli from some Healthcentres in Nasarawa-West Senatorial District, Nasarawa State, Nigeria

Molecular Characterization of Plasmid-Mediated Quinolone resistance in Urinary Escherichia coli from some Healthcentres in Nasarawa-West Senatorial District, Nasarawa State, Nigeria

Cefotaxime-, Ciprofloxacin-, and Extensively Drug-Resistant Escherichia coli O157:H7 and O55:H7 in Camel Meat

Acquisition of cephalosporin resistance genes bla_CTX‐M_‐55, bla_CTX‐M_‐65, and bla_CMY_‐2 leads to quinolone resistance in colistin‐resistant Escherichia coli harboring mcr‐1 gene

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The prevalence of plasmid-mediated quinolone resistance genes among Escherichia coli strains isolated from urinary tract infections in southwest Iran

Cited by 9 publications

References 32 publications

Molecular Characterization of Plasmid-Mediated Quinolone resistance in Urinary Escherichia coli from some Healthcentres in Nasarawa-West Senatorial District, Nasarawa State, Nigeria

Molecular Characterization of Plasmid-Mediated Quinolone resistance in Urinary Escherichia coli from some Healthcentres in Nasarawa-West Senatorial District, Nasarawa State, Nigeria

Cefotaxime-, Ciprofloxacin-, and Extensively Drug-Resistant Escherichia coli O157:H7 and O55:H7 in Camel Meat

Acquisition of cephalosporin resistance genes blaCTX‐M‐55, blaCTX‐M‐65, and blaCMY‐2 leads to quinolone resistance in colistin‐resistant Escherichia coli harboring mcr‐1 gene

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Acquisition of cephalosporin resistance genes bla_CTX‐M_‐55, bla_CTX‐M_‐65, and bla_CMY_‐2 leads to quinolone resistance in colistin‐resistant Escherichia coli harboring mcr‐1 gene