The Prevalence of netB Gene in Isolated Clostridium perfringens From Organic Broiler Farms Suspected to Necrotic Enteritis

Ezatkhah, Majid; Alimolaei, Mojtaba; Shahdadnejad, Neda

doi:10.17795/ijep35667

Cited by 2 publications

(2 citation statements)

References 21 publications

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“…Similarl studies [51,47,36] detected cpb 2 toxin gene in 75%, 74.2% and 62.6% of C. perfringens type A isolates in NE affected chickens. C. perfringens isolates were not capable of causing disease without net B gene especially it is linked with the health condition of the bird while a weak or no relationship between β 2 toxin and NE disease in birds [8,46].…”

Section: Discussionmentioning

confidence: 95%

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Toxin Genotyping of <i>C. perfringens</i> Isolated from Broiler Cases of Necrotic Enteritis

Ibrahim¹

2017

AVS

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Clostridium perfringens organisms have an economic concern in poultry production. The purpose of this study was to investigate Net B and β 2 C. perfringens positive isolates in broiler farms and their clinic-pathological effects in broiler chicks. A bacteriological examination of C. perfringens was carried upon 92 Necrotic enteritis (NE) diseased cases and 55 apparently healthy broilers of different ages in Egypt. C. perfringens type A was only recovered (49.7%). cpa gene was detected in 100% of samples with PCR technique. NE diseased cases exhibited both Net B (87.5%) and cpb 2 (75%) toxin genes. Experimentally, an intra-gut induction of Net B and β 2 C. perfringens toxins were evaluated in chicken models. The hematological studies revealed hemolytic anemia 5 days post infection (p.i) in Net B and β 2 inoculated groups (G1&G2). Leucogram revealed neutrophilia and lymphopenia 5 days p.i. A significant increase in ALT, AST, uric acid and creatinine serum levels were recorded in the infected groups at 5 th and 12 th day p.i. DNA Sequencing for net B gene revealed an amino acid replacement from glutamate into arginine at codon 379 with silent mutation was also detected at nucleotide 1134. Sequencing of both toxin genes were recorded in the gene bank for the first time in Egypt. This study pointed out that C. perfringens Net B toxin, is a new key virulent factor for the development of NE. Further studies of Net B toxiod for vaccine production could minimize the clostridial problems in broiler farms.

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Section: Discussionmentioning

confidence: 95%

“…A new discovered virulence determinant (net B) toxin recently was discovered and studied [9,45,46]. In this paper, net B toxin of C. perfringens was studied and detected in NE diseased broilers in a percentage of (46.7%) but didn't found in the isolates from healthy birds.…”

Section: Discussionmentioning

confidence: 96%

Toxin Genotyping of <i>C. perfringens</i> Isolated from Broiler Cases of Necrotic Enteritis

Ibrahim¹

2017

AVS

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Clostridium Perfringens Toxin Types Associated with Meat: Review in Iran

Masoumi¹,

Hosseini²,

Moghaddam³

et al. 2021

Iran J Med Microbiol

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Food poisoning due to Clostridium perfringens (C. perfringens) is a major food health problem, particularly in terms of meat consumption. Due to human's susceptibility to this pathogen, detection methods and prevention measures should be implemented to reduce its incidence. Several pathogenic strains of C. perfringens have been identified so far. One of the potential concerns about this bacterium is its toxin-producing characteristic that causes food poisoning. It has seven toxin types (A-G) according to the existence of four unique toxin genes. This study aimed to assess the prevalence of food poisoning caused by C. perfringens in meat and meat-derived products in Iran. We collected and categorized all the available data on this issue in Iran. Moreover, we summarized some methods used to detect toxins and genes and finally placed a prevention section for clarifying how to prevent such events. The best method for preventing such an organism's growth is by keeping foods in their normal state (hot and cold criteria) and chilling prepared foods in shallow containers as soon as possible.

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The Prevalence of netB Gene in Isolated Clostridium perfringens From Organic Broiler Farms Suspected to Necrotic Enteritis

Cited by 2 publications

References 21 publications

Toxin Genotyping of <i>C. perfringens</i> Isolated from Broiler Cases of Necrotic Enteritis

Toxin Genotyping of <i>C. perfringens</i> Isolated from Broiler Cases of Necrotic Enteritis

Clostridium Perfringens Toxin Types Associated with Meat: Review in Iran

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The Prevalence of netB Gene in Isolated Clostridium perfringens From Organic Broiler Farms Suspected to Necrotic Enteritis

Cited by 2 publications

References 21 publications

Toxin Genotyping of &lt;i&gt;C. perfringens&lt;/i&gt; Isolated from Broiler Cases of Necrotic Enteritis

Toxin Genotyping of &lt;i&gt;C. perfringens&lt;/i&gt; Isolated from Broiler Cases of Necrotic Enteritis

Clostridium Perfringens Toxin Types Associated with Meat: Review in Iran

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Toxin Genotyping of <i>C. perfringens</i> Isolated from Broiler Cases of Necrotic Enteritis

Toxin Genotyping of <i>C. perfringens</i> Isolated from Broiler Cases of Necrotic Enteritis