The prevalence of Merkel cell polyomavirus in Japanese patients with Merkel cell carcinoma

Hattori, Tomoyasu; Takeuchi, Yûkô; Takenouchi, Tatsuya; Hirofuji, Akiko; Tsuchida, Takaaki; Kabumoto, Takenori; Fujiwara, Hiroshi; Ito, Masaaki; Shimizu, Akira; Okada, Etsuko; Motegi, Sei‐ichiro; Tamura, Atsushi; Ishikawa, Osamu

doi:10.1016/j.jdermsci.2013.02.010

Cited by 32 publications

(33 citation statements)

References 51 publications

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“…The most commonly used antibody is CM2B4, which specifically detects MCPyV LTAg and not other polyomavirus T-antigens (9)(10)(11)(12)(13). Multiple reports have found IHC with CM2B4 antibody to be less sensitive than qPCR (14,16,17). Existing assays have limitations for routine clinical practice, warranting the need for development of new MCPyV detection methods.…”

Section: Introductionmentioning

confidence: 99%

Age and Gender Associations of Virus Positivity in Merkel Cell Carcinoma Characterized Using a Novel RNA In Situ Hybridization Assay

Wang

Harms

Palanisamy

et al. 2017

Clinical Cancer Research

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Purpose-Merkel cell carcinoma (MCC) is a highly aggressive neuroendocrine tumor of the skin. Merkel cell polyomavirus (MCPyV) plays an oncogenic role in the majority of MCCs. Detection of MCPyV in MCCs has diagnostic utility and prognostic potential. We investigated whether RNAscope, an RNA in situ hybridization (ISH) assay for detection of RNA transcripts in tissues, is useful for MCPyV detection.Experimental Design-We applied an RNAscope probe targeting MCPyV T antigen transcripts on tissue microarrays (TMAs) and whole tissue sections encompassing 87 MCCs from 75 patients, 14 carcinomas of other types, and benign tissues. For comparison, quantitative PCR (qPCR) was performed on 57 cases of MCC from 52 patients.Results-RNA-ISH demonstrated the presence of MCPyV in 37/75 (49.3%) cases. Notably, tumors from younger patients (< 73 years) had a significantly higher virus positivity than those from elderly patients (≥ 73 years) (64.9% vs. 34.2%, P =0.011). Female patients had a higher positive rate of MCPyV than male patients (66.7% vs. 39.6%, P =0.032). Data from both RNA- HHMI Author Manuscript HHMI Author Manuscript HHMI Author Manuscriptdetermining MCPyV status, RNAscope had 100% sensitivity and 100% specificity. There was a strong correlation between qPCR copy number and RNA-ISH product score (Spearman's correlation coefficient R 2 = 0.932, P < 0.0001).Conclusions-RNA-ISH is comparably sensitive to qPCR for detection of MCPyV and allows for correlation with tissue morphology. This study also reveals a significant association between age, gender, and MCPyV positivity.

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Section: Introductionmentioning

confidence: 99%

Age and Gender Associations of Virus Positivity in Merkel Cell Carcinoma Characterized Using a Novel RNA In Situ Hybridization Assay

Wang

Harms

Palanisamy

et al. 2017

Clinical Cancer Research

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“…Furthermore, it has been shown that JCPyV coevolved with human populations and can be used as a molecular tracer for deciphering human migrations (10). Concerning MCPyV, recent studies have suggested the existence of geographically related variants with one group composed of MCPyV strains present in Caucasians and another group comprising strains found in Asians (11)(12)(13). This hypothesis, currently based on few preliminary data, needs to be strengthened on a larger scale.…”

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confidence: 99%

Molecular Epidemiology of Merkel Cell Polyomavirus: Evidence for Geographically Related Variant Genotypes

et al. 2014

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“…Recently, the potential causative role of MCPyV has been suggested in the pathogenesis of MCC, because this virus is identified in approximately 90% of MCC cases (2). On the other hand, MCPyV is usually not detected in other skin tumours such as SCC (5).…”

Section: Discussionmentioning

confidence: 99%

“…Elderly patients with EV have a high risk of developing carcino mas in situ and invasive squamous cell carcinoma (SCC) associated with HPV infections, mainly on sunexposed skin. On the other hand, Merkel cell polyomavirus (MC PyV) is detected in most Merkel cell carcinomas (MCC) (2), which arise from Merkel cells, neuroendocrine cells in the skin. We present here a rare occurrence of MCPyV + MCC in a patient with EV.…”

Section: Short Communicationmentioning

confidence: 99%

Merkel Cell Polyomavirus-positive Merkel Cell Carcinoma in a Patient with Epidermodysplasia Verruciformis

Mizuno¹,

Kato²,

Shu³

et al. 2015

Acta Derm Venerol

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The prevalence of Merkel cell polyomavirus in Japanese patients with Merkel cell carcinoma

Cited by 32 publications

References 51 publications

Age and Gender Associations of Virus Positivity in Merkel Cell Carcinoma Characterized Using a Novel RNA In Situ Hybridization Assay

Age and Gender Associations of Virus Positivity in Merkel Cell Carcinoma Characterized Using a Novel RNA In Situ Hybridization Assay

Molecular Epidemiology of Merkel Cell Polyomavirus: Evidence for Geographically Related Variant Genotypes

Merkel Cell Polyomavirus-positive Merkel Cell Carcinoma in a Patient with Epidermodysplasia Verruciformis

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