The prevalence of human parvovirus B19 DNA and antibodies in blood donors from four Chinese blood centers

Ke, Ling; He, Miao; Li, Changqing; Liu, Yu; Gao, Lei; Yao, Fen; Li, Julin; Bi, Xingang; Lv, Yunlai; Wang, Jingxing; Hirsch, Matthew; Li, Wuping

doi:10.1111/j.1537-2995.2011.03067.x

Cited by 49 publications

(52 citation statements)

References 47 publications

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“…This prevalence is also similar to the data obtained in the USA (0.88 %) [8]. Nevertheless, the observed prevalence was higher compared to countries like Belgium (0.2 %) [9] and China (0.58 %) [10] and lower compared to Dutch blood donors (2.5 %) [11]. Such a difference is probably due to the different sample volume used in the B19V testing, demographic characteristics of the populations or regional specifications of the B19V circulation (genotypes) in a given geographic region.…”

supporting

confidence: 77%

Prevalence and Viral Load of Human Parvovirus B19 (B19V) Among Blood Donors in South-East Brazil

Slavov

Otaguiri

Covas

et al. 2015

Indian J Hematol Blood Transfus

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The infection of human parvovirus B19 (B19V) is a common event in the general population, including volunteer blood donors. In some cases it can be asymptomatic and can remain persistent for a long period of time. The objective of this study was to examine the B19V DNA prevalence and viral load in first-time volunteer blood donors. Blood samples were collected from 91 primary blood donors at the Regional Blood Center of Ribeirão Preto, Southeast Brazil. Viral detection and quantitation was performed by an in-house TaqMan Ò real-time PCR with high sensitivity. B19V DNA was detected in one male blood donor (1.0 %) and was characterized by a very low viral load (537.36 copies/mL). Our studies demonstrate that B19V DNA at low titer may be present in apparently healthy individuals. Sensitive molecular diagnostic tools can be applied for the screening of fresh blood derived products in order to prevent transfusion-transmitted B19V infection. Keywords Human parvovirus B19 Á B19V Á Viral quantitation Á Blood donorsHuman parvovirus B19 (B19V), a small icosahedral virus (19-22 nm), is the prototype member of the Erythrovirus genus (Parvoviridae family). B19V infection is a common finding in the general population and demonstrates marked seasonality. Clinical manifestations of B19V infection in children and adults may include erythema infectiosum (fifth disease) or arthropathy, nevertheless, great proportion of the infected individuals remain asymptomatic [1]. Therefore, in the general population B19V infections among blood donors can be considered a normal event and many of them have asymptomatic course [2,3]. A peculiar characteristic of B19V infection is that not always the virus is cleared and in many cases it can establish persistency in different body tissues [4]. Persistent B19V infection has been observed in immunologically normal individuals [5] including qualified blood donors [6]. The presence of persistent B19V infection in blood donors raises serious questions regarding transfusion safety of the obtained hemoderivatives, especially plasma products and packed erythrocytes.Moreover, B19V is highly resilient to almost all virus inactivation procedures [7], causes infections with a high viral load and these properties predispose to easy viral contamination of the blood products. The relevance of the low B19V load in asymptomatic donors and for the respective recipients of blood is still unclear. Therefore, the objective of this study was to evaluate B19V DNA prevalence and viral load (VL) during 1 month blood collection in a non-metropolitan blood transfusion center in Southeast Brazil (Ribeirão Preto city, Western part of the São Paulo State).Ninety one peripheral blood samples were collected from 91 first time volunteer blood donors (mean age 35.7 years, 18-65 years, 55 % males). All of them were approved on the preliminary interview for blood donation & Svetoslav Nanev Slavov

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confidence: 77%

Prevalence and Viral Load of Human Parvovirus B19 (B19V) Among Blood Donors in South-East Brazil

Slavov

Otaguiri

Covas

et al. 2015

Indian J Hematol Blood Transfus

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“…The study indicated a 0.58% genome DNA prevalence rate among the blood donor population [26], but the study was not large enough to define range of viral titers, as samples with very high titers are relatively rare. In this study, we investigated B19V DNA contamination in 142 plasma pools from two Chinese manufacturers.…”

Section: Discussionmentioning

confidence: 99%

“…Hot-start amplification was performed under the following conditions: 1 cycle of 95°C for 10 min; 45 cycles of 95°C for 15 s, 60°C for 30 s, and 72°C for 30 s; and a final cycle of 95°C for 15 s, 60°C for 15 s, and then gradually increased to 95°C in 30 min at a ramp rate of 2%. Detailed Q-PCR procedures have been previously described [26]. Q-PCR detected B19V DNA positive samples were confirmed by nested PCR (nPCR) using the conserved primers located in the NS1 region described previously [25].…”

Section: Methodsmentioning

confidence: 99%

Parvovirus B19V DNA contamination in Chinese plasma and plasma derivatives

Zhang

et al. 2012

J Transl Med

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BackgroundTo ensure the safety of plasma derivatives, screening for human parvovirus B19V genomic DNA in donated plasma using a pooling strategy is performed in some countries. We investigated the prevalence of B19V DNA and anti-B19V antibodies in Chinese plasma pools, plasma derivatives and plasma donations to evaluate the risk posed by B19V.MethodsUsing a Q-PCR assay developed in-house, we tested for B19V genomic DNA in 142 plasma pools collected between January 2009 and June 2011 from two Chinese blood products manufacturers. Plasma derivatives collected between 1993–1995 (10 batches of albumin, 155 batches of intravenous immunoglobulin, IVIG) and 2009–2011 (50 batches of albumin, 54 batches of IVIG, 35 batches of factor VIII, 7 batches of fibrinogen, and 17 batches of prothrombin complex concentrate, PCC) were also tested for B19V contamination. In addition, B19V genome prevalence in minipools(including 90 individual donations) of 49680 individual plasma samples collected between August 2011 and March 2012 by a single Chinese manufacturer was investigated. IgM/IgG was also investigated in plasma pools/derivatives and in minipools with B19V-DNA titers above 1x104 and 1x106 geq/mL using B19 ELISA IgM/IgG assay(Virion-Serion, Würzburg, Germany), respectively.ResultsB19V-DNA was detected in 54.2% of plasma pools from two Chinese blood product manufacturers; among recently produced blood products, B19V was detected in 21/54 IVIG samples, 19/35 factor VIII samples, 6/7 fibrinogen samples, and 12/17 PCC samples, but not in albumin samples. The levels of B19V-DNA in these samples varied from 102-107 geq/mL. In samples with >104 geq/mL genome DNA, B19V-specific IgG was also found in all corresponding plasma pools and IVIG, whereas none was detected in the majority of other plasma derivatives. Screening of plasma donations indicated that most minipools were contaminated with B19V-DNA (102-108 geq/mL) and one donation had 1.09 × 1010 geq/mL B19V genomic DNA along with a non-classical IgG/IgM profile.ConclusionsDespite the implementation of some inactivation/removal methods designed to prevent viral contamination, B19V DNA was detectable in Chinese plasma pools and plasma derivatives. Thus, the introduction of B19V screening and discard donation with high viramic concentration for Chinese plasma donors would be desirable.

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“…To measure the mRNA levels of HBV, Timp-1 , and Tgf-β1 , total RNA was isolated using a NucleoSpinRNA II kit (Macherey Nagal,GmbH & Co. KG, Germany) and reverse transcribed using a First Strand cDNA Synthesis Kit (Toyobo, Japan). All of the qPCR reactions were performed in triplicate in 96-well optical reaction plates using an ABI 7900 Sequence Detection System (Applied Biosystems, Foster City, CA) and SYBR Green I PCR mix (Roche Diagnostics, Indianapolis, IN) as previously described [31]. The primer sequences are shown in the S1 Table.…”

Section: Methodsmentioning

confidence: 99%

Adeno-Associated Virus Vector Mediated Delivery of the HBV Genome Induces Chronic Hepatitis B Virus Infection and Liver Fibrosis in Mice

et al. 2015

PLoS ONE

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Liver cirrhosis and hepatocellular carcinomas are major health problems of chronic hepatitis B virus (HBV) infection. To date, rare model has reproduced liver fibrosis associated with long-term HBV infection which in turn has hindered both the understanding of HBV biology and the development of new treatment options. Here, using adeno-associated virus serotype 8 (AAV8) mediated delivery of a 1.2-kb HBV genome, we successfully generated a chronic HBV infectious mouse model that presents the associated liver fibrosis observed following human infection. After AAV8/HBV1.2 vector administration, mice demonstrated effective HBV replication and transcription which resulted in HBV antigen expression and viremia over 6 months. Although no obvious acute inflammatory response was noted, these mice still developed chronic liver disease and hepatic fibrogenesis as demonstrated by increased ground glass-like hepatocytes, an increasing trend of collagen deposition and upregulated fibrosis markers, including type I collagen, type III collagen, tissue inhibitor of metalloproteinase (TIMP), and transforming growth factor-β1(TGF-β1). Taken together, AAV-mediated HBV gene delivery to the mouse liver, induced HBV persistent infection accompanied by liver fibrosis which can serve as a model for investigating the precise mechanisms underlying liver fibrosis following chronic HBV infection as well as for the potential development of novel therapeutics.

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The prevalence of human parvovirus B19 DNA and antibodies in blood donors from four Chinese blood centers

Abstract: Whether B19 NAT screening of blood and blood products should be launched in China, larger studies are needed to facilitate an informed decision.

Cited by 49 publications

References 47 publications

Prevalence and Viral Load of Human Parvovirus B19 (B19V) Among Blood Donors in South-East Brazil

Prevalence and Viral Load of Human Parvovirus B19 (B19V) Among Blood Donors in South-East Brazil

Parvovirus B19V DNA contamination in Chinese plasma and plasma derivatives

Adeno-Associated Virus Vector Mediated Delivery of the HBV Genome Induces Chronic Hepatitis B Virus Infection and Liver Fibrosis in Mice

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