The Prevalence of Hepatitis C Virus Core Amino Acid 70 Substitution and Genotypes of Polymorphisms Near the IFNL3 Gene in Iranian Patients With Chronic Hepatitis C

Kadjbaf, Danesh; Keshvari, Maryam; Alavian, Seyed Moayed; Pouryasin, Ali; Behnava, Bita; Salimi, Shima; Mehrnoush, Leila; Elizee, Pegah Karimi; Sharafi, Heidar

doi:10.5812/hepatmon.37011

Cited by 9 publications

(8 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Iran, HCV genotype 1 is the most prevalent HCV isolate (17,18). Moreover, HCV genotype 1 was the most isolated genotype from Iranian thalassemia patients with HCV infection (19). However, a previous study in Sistan and Baluchistan found HCV genotype 3 as the dominant genotype in children and adolescents with thalassemia major (20).…”

Section: Discussionmentioning

confidence: 98%

Hepatitis B and Hepatitis C in Patients with Inherited Disorders of Hemoglobin in Sistan and Baluchistan Province, Iran

Khosravi

Sharafi

Moghadam

et al. 2018

Arch Clin Infect Dis

Self Cite

View full text Add to dashboard Cite

Background: The hepatitis C virus (HCV) and hepatitis B virus (HBV) infections are significant concerns in patients with inherited disorders of hemoglobin (IDH). Objectives: This study aimed to assess the prevalence of HCV and HBV infections in a large cohort of patients with IDH in Sistan and Baluchistan province, Iran. Methods: In this retrospective study, 2391 patients with IDH in Sistan and Baluchistan were enrolled. The anti-HCV antibody (HCV Ab) and hepatitis B surface antigen (HBsAg) were assessed using ELISA. Results: A total of 2387 patients with a mean age of 12.7 years were included in the final analysis. Most of the patients were residents of Zahedan city (28.1%) and suffered beta-thalassemia major (99.2%). The prevalence of HCV Ab was 5.87% (95% CI = 5.0%-6.89%) and HBsAg was detected in 0.29% (95%CI = 0.14%-0.6%) of the patients. The prevalence of HCV Ab was associated with the age of patients, and there was a great difference in the prevalence of HCV Ab between patients with IDH who born before and after 1996 (17.2% vs. 5.1%; P < 0.05). Among 101 patients with positive results for HCV Ab who were tested for HCV RNA, 53 (52.5%; 95% CI = 42.8%-61.9%) had HCV RNA in their serum samples. Moreover, 40 patients had results for HCV genotyping including 29 (72.5%) HCV genotype 3 and 11 (27.5%) HCV genotype 1. Conclusions: Hepatitis C had a high seroprevalence of 5.87% in patients with IDH in Sistan and Baluchistan while hepatitis B had a low seroprevalence of 0.29% that is unusual in a region with > 3% prevalence of hepatitis B in the general population.

show abstract

Section: Discussionmentioning

confidence: 98%

Hepatitis B and Hepatitis C in Patients with Inherited Disorders of Hemoglobin in Sistan and Baluchistan Province, Iran

Khosravi

Sharafi

Moghadam

et al. 2018

Arch Clin Infect Dis

Self Cite

View full text Add to dashboard Cite

show abstract

“…The different pattern of HCV infection in Iranian hemophiliac patients has been clearly defined. Previously, it was shown that HCV-1b is more frequently observed in Iranian hemophiliac patients than other Iranian HCV infected groups (57). This may indicate the possible similarity of HCV-1b of Iranian and European isolates and suggests an infection through blood products such as clotting factors imported to Iran.…”

Section: Discussionmentioning

confidence: 99%

Molecular Tracing of Hepatitis C Virus Genotype 1 Isolates in Iran: A NS5B Phylogenetic Analysis with Systematic Review

et al. 2016

Self Cite

View full text Add to dashboard Cite

ContextHepatitis C virus (HCV) is characterized by a high degree of genetic heterogeneity and classified into 7 genotypes and different subtypes. It heterogeneously distributed through various risk groups and geographical regions. A well-established phylogenetic relationship can simplify the tracing of HCV hierarchical strata into geographical regions. The current study aimed to find genetic phylogeny of subtypes 1a and 1b of HCV isolates based on NS5B nucleotide sequences in Iran and other members of Eastern Mediterranean regional office of world health organization, as well as other Middle Eastern countries, with a systematic review of available published and unpublished studies.Evidence AcquisitionThe phylogenetic analyses were performed based on the nucleotide sequences of NS5B gene of HCV genotype 1 (HCV-1), which were registered in the GenBank database. The literature review was performed in two steps: 1) searching studies evaluating the NS5B sequences of HCV-1, on PubMed, Scopus, and Web of Science, and 2) Searching sequences of unpublished studies registered in the GenBank database.ResultsIn this study, 442 sequences from HCV-1a and 232 from HCV-1b underwent phylogenetic analysis. Phylogenetic analysis of all sequences revealed different clusters in the phylogenetic trees. The results showed that the proportion of HCV-1a and -1b isolates from Iranian patients probably originated from domestic sources. Moreover, the HCV-1b isolates from Iranian patients may have similarities with the European ones.ConclusionsIn this study, phylogenetic reconstruction of HCV-1 sequences clearly indicated for molecular tracing and ancestral relationships of the HCV genotypes in Iran, and showed the likelihood of domestic origin for HCV-1a and various origin for HCV-1b.

show abstract

“…On samples with con rmed positive antibody, quantitative HCV RNA test (The Artus HCV RG RT-PCR Kit, Qiagen) and genotyping were performed by reverse transcription-polymerase chain reaction (RT-PCR) followed by sequencing (26). Sample collection procedures have been previously detailed elsewhere (27).…”

Section: Sample Collectionmentioning

confidence: 99%

Continuum of Hepatitis C Care Cascade in Prison and Following Release in the Direct-acting Antivirals Era

Hariri

Sharafi

Sheikh

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background People with criminal justice involvement contribute remarkably to the rising hepatitis C virus (HCV) burden; however, the continuum of care is a major barrier to prison-based programs. We aimed to evaluate a comprehensive HCV care model in an Iranian provincial prison. Methods Between 2017–2018, in the Karaj Central Prison, newly admitted male inmates received HCV antibody testing and venipuncture for RNA testing (antibody-positive only). Participants with positive RNA underwent direct-acting antiviral (DAA) therapy (Sofosbuvir/Daclatasvir). Sustained virological response was evaluated at 12 weeks post-treatment (SVR12). Results Overall, from 3,485 participants, 182 (5.2%) and 117 (3.4%) tested positive for HCV antibody and RNA, respectively. Among 116 patients who were eligible for treatment, 24% (n = 28) were released before treatment and 72% (n = 83) initiated DAA therapy, of whom 81% (n = 67/83) completed treatment in prison and the rest were released. Of total released patients, 68% (n = 30/44) were linked to care in community, and 70% (n = 21/30) completed treatment, including 60% (n = 12/20) and 90% (n = 9/10) among those who were released before and during treatment, respectively. The overall HCV treatment uptake and completion were 89% (n = 103/116) and 85% (n = 88/103), respectively. From people who completed treatment, 43% (n = 38/88) attended for response assessment and all were cured (SVR = 100%). Conclusions Integrated HCV care models are highly effective and can be significantly strengthened by post-release interventions. The close collaboration of community and prison healthcare systems is crucial to promote high levels of adherence to treatment. Future studies should investigate the predictors of engagement with HCV care following release.

show abstract

The Prevalence of Hepatitis C Virus Core Amino Acid 70 Substitution and Genotypes of Polymorphisms Near the IFNL3 Gene in Iranian Patients With Chronic Hepatitis C

Cited by 9 publications

References 63 publications

Hepatitis B and Hepatitis C in Patients with Inherited Disorders of Hemoglobin in Sistan and Baluchistan Province, Iran

Hepatitis B and Hepatitis C in Patients with Inherited Disorders of Hemoglobin in Sistan and Baluchistan Province, Iran

Molecular Tracing of Hepatitis C Virus Genotype 1 Isolates in Iran: A NS5B Phylogenetic Analysis with Systematic Review

Continuum of Hepatitis C Care Cascade in Prison and Following Release in the Direct-acting Antivirals Era

Contact Info

Product

Resources

About