The prevalence of dogs with lymphocyte proliferative responses to food allergens in canine allergic dermatitis

Kawano, Koji; Oumi, K; Ashida, Yosinori; Horiuchi, Yutaka; Mizuno, Takuya

doi:10.2478/pjvs-2013-0104

Cited by 16 publications

(24 citation statements)

References 6 publications

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“…Previous studies have shown that ALD constitutes 14 to 25% of CAD, which is similar to that of intrinsic AD in humans (20%) (Tokura 2010, Fujimura 2011, Kabashima et al 2013, Kawano et al 2013, Suto et al 2014. From what we know of the mode of action of CsA, it may be more effective against intrinsic AD, because IL-2 is a Th1 related cytokine and intrinsic AD in humans is Th1-dominant.…”

Section: Discussionmentioning

confidence: 81%

Cyclosporin A Treatment in Intrinsic Canine Atopic Dermatitis (Atopic-like Dermatitis): Open Trial Study

Fujimura¹,

Ishimaru²

2016

Polish Journal of Veterinary Sciences

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In this study, dogs were separated into two groups and treated with immunosuppressant (Cyclosporin A: CsA). The first group was the canine atopic dermatitis (CAD) group, which is similar to extrinsic atopic dermatitis (AD) in humans (treated with a CsA dose of 2.5-5.5 mg/kg, n=8), and the second group was the canine atopic-like dermatitis (ALD) group, which is similar to intrinsic AD in humans (treated with a CsA dose of 2.5-6.5 mg/kg, n=14). The canine atopic dermatitis extent and severity index (CADESI)-4 was evaluated before treatment (PRE) and after treatment (POST) to assess the effectiveness of CsA for the two groups. In the CAD group, CADESI-4 showed no change (PRE:79±29, POST:77±28) and out of the eight dogs, no dogs showed complete remission, three dogs showed partial remission, and five dogs showed no effect. Whereas in the ALD group, CADESI-4 showed a significant reduction (PRE: 61±42, POST: 32±25, p<0.01) and out of the 14 dogs, 11 dogs showed complete remission, two dogs showed partial remission, and one dog showed no effect. The results indicate that the immunosuppressant showed effectiveness for the dogs diagnosed with ALD. One dog had to be treated for a year and eight months, which was the longest period in the study, this dog presented with hyperplasia of the lymphoidgland and mammary tumor.

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Section: Discussionmentioning

confidence: 81%

Cyclosporin A Treatment in Intrinsic Canine Atopic Dermatitis (Atopic-like Dermatitis): Open Trial Study

Fujimura¹,

Ishimaru²

2016

Polish Journal of Veterinary Sciences

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“…The PBMC's utilized in this study were sent by veterinarians for a previously described standard lymphocyte proliferation assay [5,12,25], and after the assay was completed, the remaining cells were used to examine T-lymphocyte responses to the hydrolyzed diets. Therefore, we did not have access to the history and clinical features of the dogs and blood samples.…”

Section: Discussionmentioning

confidence: 99%

“…Another report evaluating clinical cases of dogs with chronic dermatitis and suspected food hypersensitivity confirmed this trend [5]. In addition, a study conducted on dogs with gastrointestinal symptoms clinically diagnosed as inflammatory bowel disease, found peripheral lymphocytes reactive to at least one food antigen in all the animals [12]. Flow cytometry analysis revealed that the type IV lymphocytes associated with hypersensitivity were CD4 + helper T-lymphocytes [5,21].…”

mentioning

confidence: 86%

“…As previously reported [5,12,25], lymphocyte proliferation of PBMC's isolated (by veterinarians in private animal hospitals) from dogs with suspected food hypersensitivity, was investigated in a routine assay with 18 types of food antigens in a commercial laboratory (Animal Allergy Clinical Laboratories, Inc., Sagamihara, Japan). The food allergens for the standard lymphocyte assay were extracted from beef, pork, chicken, egg white, egg yolk, milk, wheat, soybean, corn, lamb, turkey, duck, salmon, cod fish, catfish, potato, and rice, and all were prepared and purchased from Greer Laboratories Inc. as previously described [5,12,25]. However, capelin was prepared by homogenization of the dried fish body in PBS.…”

Section: Lymphocyte Stimulationmentioning

confidence: 99%

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Hydrolyzed diets may stimulate food-reactive lymphocytes in dogs

Masuda¹,

Sato²,

Tanaka³

et al. 2020

The Journal of Veterinary Medical Science

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Hydrolyzed proteins are often prescribed for dogs with food hypersensitivity in food elimination programs. However, the potential of these diets to stimulate lymphocyte-mediated hypersensitivity is currently unknown. In this study, two commercially available hydrolyzed diets for dogs, D-1 (Aminopeptide Formula Dry, Royal Canin Japon, Tokyo, Japan), and D-2 (Canine z/d Ultra Dry, Hill's-Colgate (Japan) Ltd., Tokyo, Japan), were analyzed to identify residual proteins or peptides, as well as activated helper T-lymphocyte reactions in dogs with suspected food hypersensitivity. Proteins and peptides with molecular weights >1 kDa (majority 1.5-3.5 kDa) were detected in both diet extracts with sodium dodecyl sulfate polyacrylamide gel electrophoresis, and size exclusion chromatography. When peripheral blood mononuclear cells (PBMC's) from 316 dogs with suspected food allergies were cultured with hydrolyzed diet extracts, flow cytometry analysis revealed detectable levels of CD25 low helper T-lymphocytes stimulated by D-1 and D-2 in 91 of 316, (28.8%), and 75 of 316 (23.7%) samples, respectively. These data indicated that the extracts contained proteins or peptides large enough to activate the lymphocytes. The percentages of CD25 low helper T-lymphocytes stimulated by D-1 and D-2 extracts increased to 38.7% and 29.6%, respectively, in 186 of the original 316 samples (186/316, 58.9%), also reactive to poultry-related antigens. Thus, both poultry-related antigens, and D-1 and D-2 diet extracts may activate helper T-lymphocytes. These results demonstrate that hydrolyzed diets may contain proteins that stimulate helper T-lymphocytes, and may not be effective for treating all dogs with food hypersensitivity.

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“…Ryota Asahina 1) , Takafumi Osumi 2) , Takuya Mizuno 3) , Yuri Oshima 4) , Michiyo Kadoya 5) , Tae Murai 6) , Kentaro Yamagishi 7) , Koji Nishifuji 2) , Masahiko Nagata 8) , Sadatoshi…”

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An attempt to develop guidelines for the diagnosis and treatment of canine atopic dermatitis: current status and issues

et al. 2019

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The international clinical guideline for canine atopic dermatitis (CAD) was published by the International Committee on Allergic Diseases of Animals in 2015. Unfortunately, it does not fully cover the situations in Japan due to differences in veterinary practice between Japan and the rest of world. The Guideline Committee of the Japanese Society of Veterinary Dermatology (JSVD) attempted to develop guidelines for the diagnosis and treatment of CAD in 2016 and 2017, respectively. However, these attempts were unsuccessful because of a lack of clinical evidence. The most critical issues identified were the clarification of the clinical features of CAD among the breeds in which it commonly occurs in Japan, and the establishment of new methods for evaluating its severity. In particular, it is important to promote clinical studies supported by JSVD, in order to establish suitable biomarkers that can be used to objectively evaluate the therapeutic effect of treatments for CAD.

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The prevalence of dogs with lymphocyte proliferative responses to food allergens in canine allergic dermatitis

Cited by 16 publications

References 6 publications

Cyclosporin A Treatment in Intrinsic Canine Atopic Dermatitis (Atopic-like Dermatitis): Open Trial Study

Cyclosporin A Treatment in Intrinsic Canine Atopic Dermatitis (Atopic-like Dermatitis): Open Trial Study

Hydrolyzed diets may stimulate food-reactive lymphocytes in dogs

An attempt to develop guidelines for the diagnosis and treatment of canine atopic dermatitis: current status and issues

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