The prevalence of Borrelia in Ixodes persulcatus in southeastern Kazakhstan

Zhigailov, Andrey V.; Neupokoyeva, Alena S.; Maltseva, Elina R.; Perfilyeva, Yuliya V.; Bissenbay, Akerke O.; Turebekov, Nurkeldi; Frey, Stefan; Eßbauer, Sandra; Abdiyeva, Karlygash; Ostapchuk, Yekaterina O.; Berdygulova, Zhanna A.; Aimbetov, Rakhan; Naizabayeva, Dinara A.; Dmitrovskiy, Andrey; Skiba, Yuriy A.; Mamadaliyev, Seidigapbar M.

doi:10.1016/j.ttbdis.2021.101716

Cited by 4 publications

(5 citation statements)

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“…The presented study revealed a zonal distribution of different spirochete species from the Borreliaceae family in Northern Poland not only by the presence or absence of particular species but also by differences in infection rates that correlate with different risks of human and animal infection. Similar differences in the distribution and infection rates are observed in other European studies [45][46][47]51,[58][59][60] but also in the case of different Borreliaceae species characteristics for Asia [61,62] and North America [63][64][65]. The cause of such zonality may be host specific, especially in the case of Borreliella species [66], and due to the differential availability in the studied area but not the climatic conditions which are similar for the entire area of research.…”

Section: Discussionsupporting

confidence: 79%

“…In this study, markers were applied to allow for the detection of all the representatives of the Borreliaceae family in a nested PCR procedure with subsequent species identification by restriction analysis or by discrimination of the PCR product length. This procedure allows for avoiding results with undefined Borreliella species that occur in the case of using less variable molecular markers for standard PCR or speciesspecific probes for real-time PCR procedure [53,56,60,61,68]. The approach used in this study ensures identifying all PCR products so it allows for the assessment of the real representation of particular spirochete species in examined tick populations.…”

Section: Discussionmentioning

confidence: 99%

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Genetic Diversity of Borreliaceae Species Detected in Natural Populations of Ixodes ricinus Ticks in Northern Poland

Wodecka

Kolomiiets

2023

Life

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In Europe, Ixodes ricinus tick is the vector of Lyme disease spirochetes and their relatives (Borreliella genus) and Borrelia miyamotoi. However, a newly described tick I. inopinatus with similar biological features and separated from I. ricinus may act as a vector for different Borrelia species. To date, eleven Borreliella species were detected in the natural populations of I. ricinus. Recently, two North American species have been detected in ticks parasitizing bats and red foxes in Europe, i.e., B. lanei and B. californiensis pointing to the necessity for searching for them in natural tick populations. In this study, using the coxI molecular marker only I. ricinus was identified in field-collected ticks with the exception of individual specimens of Haemaphysalis concinna. Using the flaB gene and mag-trnI intergenic spacer as molecular markers 14 Borreliaceae species have been detected with various frequencies in different parts of northern Poland. Among infected ticks, the most frequent were Borreliella (Bl.) afzelii (29.4%) and Bl. garinii (20.0%), followed by Bl. spielmanii, Bl. valaisiana, Bl. lanei, Bl. californiensis, B. miyamotoi, Bl. burgdorferi, Bl. carolinensis, Bl. americana, B. turcica, Bl. lusitaniae, Bl. bissettiae and Bl. finlandensis. Three of the above-mentioned species, i.e., Bl. lanei, Bl. californiensis and B. turcica were detected in this study for the first time in the natural ixodid tick population in Europe. The existence of the newly detected spirochetes increases their total diversity in Europe and points to the necessity of careful identification and establishment of the actual distribution of all Borreliaceae species transmitted by I. ricinus.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Genetic Diversity of Borreliaceae Species Detected in Natural Populations of Ixodes ricinus Ticks in Northern Poland

Wodecka

Kolomiiets

2023

Life

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“…A collection of samples for the examination of Orthohantaviruses with regard to their role concerning infections in acute patients was performed in eight hospitals in 2018 and 2019. Included in the study were patients presenting with either fever and/or abdominal pain and/or feeling seriously ill and/or with renal insufficiency ( 19 ). The results of this study will be obtained in the following phase of the project.…”

Section: Scientific Output Of the Projectmentioning

confidence: 99%

“…The first of these took place in 2014-15 on patients with fever of unknown origin in 13 hospitals in Almaty and Kyzylorda oblast. Fevers of unknown origin (FUO) are frequently occurring in low and middle income countries and are often a result of incomplete diagnostic setups (19,24).…”

Section: Scientific Output Of the Project Peer Reviewed Publicationsmentioning

confidence: 99%

Eight Years of Collaboration on Biosafety and Biosecurity Issues Between Kazakhstan and Germany as Part of the German Biosecurity Programme and the G7 Global Partnership Against the Spread of Weapons and Materials of Mass Destruction

Peintner

Wagner

Shin³

et al. 2021

Front. Public Health

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In 2013, the German Federal Foreign Office launched the German Biosecurity Programme with the aim to minimise risks associated with biological substances and pathogens. In this context, the German-Kazakh Network for Biosafety and Biosecurity was established in 2013 and constitutes a successful collaboration between Kazakh and German biomedical organisations, under the co-management of the Bundeswehr Institute of Microbiology (IMB), and the Deutsche Gesellschaft für Internationale Zusammenarbeit (GIZ) GmbH. Ever since then, a network of scientists, stake holders and policymakers has been established, aiming to work on highly pathogenic, potential biological warfare agents with the focus on biosafety and biosecurity, surveillance, detection and diagnostics, networking and awareness raising of these agents in Kazakhstan. Over the past 8 years, the project members trained four PhD candidates, organised over 30 workshops and trainings with more than 250 participants and conducted more than 5,000 PCR assays and 5,000 serological analyses for surveillance. A great success was the description of new endemic areas for Orthohantaviruses, the mixture of two Crimean-Congo haemorrhagic fever virus genetic clusters, new foci and genetic information on tick-borne encephalitis virus and rickettsiae in Kazakh oblasts. The latter even led to the description of two new genogroups. Furthermore, joint contributions to international conferences were made. In this report, we summarise the evolution of the German-Kazakh Network for Biosafety and Biosecurity and critically reflect on the strengths and possible weaknesses. We were able to establish a viable network of biosafety and biosecurity shareholders and to accomplish the aims of the German Biosecurity Programme to lower biosecurity risks by increased awareness, improved detection and diagnostic methods and surveillance. Further, we reflect on forthcoming aspects to lead this interstate endeavour into a sustainable future.

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“…Similarly, relapsing fevers (RF) are infectious, neglected, and emerging diseases in the Americas and in some African countries, caused by 21 recognized species [4,25,26]. Vectors involved in the transmission cycle of relapsing fevers are mainly soft ticks of the genus Ornithodoros (Argasidae), hard ticks of the genera Amblyomma, Dermacentor, Ixodes, and Rhipicephalus, the human body louse or clothing louse Pediculus humanus humanus, the latter vector of Borrelia recurrentis [4,5,[27][28][29][30][31][32]. Borrelia of the RFG exhibit transovarial and transstadial transmission in ticks, highlighting the importance of these vectors in the enzootic and epizootic cycles of the disease [4,29,32].…”

Section: Introductionmentioning

confidence: 99%

Is Borrelia burgdorferi Sensu Stricto in South America? First Molecular Evidence of Its Presence in Colombia

et al. 2022

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The genus Borrelia encompasses spirochetal species that are part of three well-defined groups. Two of these groups contain pathogens that affect humans: the group causing Lyme disease (LDG) and the relapsing fever group (RFG). Lyme disease is caused by Borrelia burgdorferi s.l., which is distributed in the Northern Hemisphere, and relapsing fevers are caused by Borrelia spp., which are found in temperate and tropical countries and are an emerging but neglected pathogens. In some departments of Colombia, there are records of the presence of Borrelia sp. in humans and bats. However, little is known about the impact and circulation of Borrelia spp. in the country, especially in wildlife, which can act as a reservoir and/or amplifying host. In this context, the objective of our research was to detect and identify the Borrelia species present in wild mammals in the departments of Caldas and Risaralda in Colombia. For morphological detection, blood smears and organ imprints were performed, and molecular identification was carried out through a nested PCR directed on the flagellin B (flaB) gene. A total of 105 mammals belonging to three orders (Chiroptera, Didelphimorphia and Rodentia) were analyzed, of which 15.24% (n = 16) were positive for Borrelia. Molecularly, the presence of Borrelia burgdorferi s.s. in lung tissues of Thomasomys aureus and blood of Mus musculus (Rodentia) was detected, with 99.64 and 100% identity, respectively. Borrelia sp. genospecies from a clade branch of a bat-associated LDG sister group were identified in seven individuals of bat species, such as Artibeus lituratus, Carollia brevicauda, Sturnira erythromos, and Glossophaga soricina. Furthermore, two Borrelia genospecies from the RFG in seven individuals of bats (A. lituratus, Artibeus jamaicensis, Platyrrhinus helleri, Mesophylla macconnelli, Rhynchonycteris naso) and rodents (Coendou rufescens, Microryzomys altissimus) were documented. Additionally, the presence of a spirochete was detected by microscopy in the liver of a Sturnira erythromos bat specimen. These results contain the first molecular evidence of the presence of B. burgdorferi s.s. in South America, which merits the need for comprehensive studies involving arthropods and vertebrates (including humans) in other departments of Colombia, as well as neighboring countries, to understand the current status of the circulation of Borrelia spp. in South America.

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The prevalence of Borrelia in Ixodes persulcatus in southeastern Kazakhstan

Cited by 4 publications

References 21 publications

Genetic Diversity of Borreliaceae Species Detected in Natural Populations of Ixodes ricinus Ticks in Northern Poland

Genetic Diversity of Borreliaceae Species Detected in Natural Populations of Ixodes ricinus Ticks in Northern Poland

Eight Years of Collaboration on Biosafety and Biosecurity Issues Between Kazakhstan and Germany as Part of the German Biosecurity Programme and the G7 Global Partnership Against the Spread of Weapons and Materials of Mass Destruction

Is Borrelia burgdorferi Sensu Stricto in South America? First Molecular Evidence of Its Presence in Colombia

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