The Prevalence of Atypical Pneumonia Caused by Mycoplasma pneumoniae (P1 gene) in Patients with Respiratory Infections by Culture and Molecular PCR Methods in Tehran, Iran

Abstract

Background: Atypical pneumonia is an upper and lower respiratory tract infection. Mycoplasma pneumoniae is a major cause of community-acquired pneumonia (CAP). Objectives: The present study aimed to determine the prevalence of atypical pneumonia caused by M. pneumonia by culture and molecular PCR methods in Tehran. Methods: In the present study, 102 samples of throat swab were collected from patients with respiratory infections. All samples were cultured in liquid PPLO Broth And solid PPLO agar media (1% glucose and 20% horse serum). The PCR technique with specific primers was implemented after culture and genome extraction through phenol-chloroform technique. Results: In this study, 27 (26.47%) colonies of Mycoplasma were isolated on PPLO agar medium. Using specific primers, it was found that 33 samples (32.4%) were positive in terms of Mycoplasma genus and 14 samples (13.7%) were positive for the presence of M. pneumonia. Conclusions: Mycoplasma pneumonia is a pathogen that causes respiratory tract infections in humans. Molecular PCR method is a quick and sensitive technique that has higher sensitivity and specificity than other methods. The obtained results may contribute to the specific treatment of some patients with symptoms of respiratory infections. Informed Consent:Patients were recruited through a complete and informed questionnaire, followed by a complete description of the research and complete patient satisfaction.

The Prevalence of 23S rRNA Mutations in ML-Resistant M. pneumoniae Isolates to Clarithromycin in Patients with Respiratory Infections

“…Although M. pneumoniae is a self-limiting disease, some patients complain of progressive disease despite courses of antibiotic therapy (7)(8)(9). Macrolides (MLs) are used as the first-line treatment of M. pneumoniae infection (1)(2)(3)(4)(5)(6)(7)(8)(9)(10). The duration of treatment of infections with ML-resistant M. pneumoniae (MRMP) is longer compared to treatment-sensitive infections (11)(12).…”

Molecular Detection and Evaluation of MLـ Resistance M. Pneumoniae Associated with Mutation in 23S RNA Gene among Iranian Patients with Respiratory Infections

“…Mycoplasma pneumoniae (M. pneumoniae) is one of the common causes of upper and lower respiratory tract infections and is a major cause of community-acquired pneumonia (CAP) and atypical pneumonia which is responsible for mortality and morbidity among children and adults (1)(2)(3)(4). Since M. pneumoniae lacks a cell wall, the infection is primarily treated with macrolide antibiotics (MLs), tetracycline, and fluoroquinolones (5)(6)(7).…”