The Prevalence of Abnormally Increasing Proportion of INF-γ/IL-4 in Peripheral Blood Among Patients with Unexplained Recurrent Spontaneous Abortion

Abstract

Problem: To retrospectively observe the abnormally elevated proportion of INF-γ / IL-4 in peripheral blood among patients with recurrent spontaneous abortion, and to evaluate the application value of flow cytometry in detecting peripheral blood INF-γ / IL-4 in early etiological screening of patients with recurrent spontaneous abortion. Method of Study: A total of 77 patients with recurrent spontaneous abortion who were admitted to the Department of Obstetrics and Gynecology of The General Hospital of the People's Liberation Army of China from January 2021 to December 2021 were selected. All patients were women who had experienced two or more consecutive spontaneous abortions before 28 weeks of gestation but had not achieved clinical pregnancy; The peripheral blood of 58 women without recurrent spontaneous abortion history was selected as the control group. The levels of CD3 + CD8-INF-γ and IL-4 in peripheral blood lymphocytes were measured, and the ratio of INF-γ / IL-4 was calculated. Results: The ratio of INF-γ / IL-4 in peripheral blood of patients with rrecurrent spontaneous abortion was significantly higher compared to the control group, and the difference was statistically significant (P=0.000, < 0.001). The difference in the ratio between the two groups was caused by abnormally high INF-γ levels in the recurrent spontaneous abortion group (P=0.000, < 0.001), there was no statistical difference in IL-4 level between the two groups. The cutoff value of INF-γ / IL-4 in peripheral blood was 6.86, and the reference range was 1.28-8.49. The AUC of the detection method was 0.75 (P=0.000, < 0.001), has high clinical diagnostic value. According to the reference range, 33.77% of patients with recurrent spontaneous abortion had abnormally elevated INF-γ / IL-4 ratio. Conclusions: Compared with the control group, INF-γ / IL-4 ratio of peripheral blood in patients with recurrent spontaneous abortion was abnormally higher, and the carrier rate was 33.77%. Flow cytometry has high clinical value in detecting INF-γ / IL-4 ratio in peripheral blood.