We aimed to investigate the prevalence of true rectocele and obstructed defecation (oD) in patients with pelvic organ prolapse (POP), to investigate the correlation between true rectocele and OD, and to understand the diagnostic value of translabial ultrasound (tLUS) in the diagnosis of true rectocele. the patients who scheduled for pop surgery were enrolled in this study. patients who had previous reconstructive pelvic surgery or repair of rectocele were excluded. Birmingham Bowel and Urinary symptoms questionnaires and Longo's obstructed defecation syndrome scoring system were used to assess the bowel symptoms of patients. tLUS was used to evaluate anatomical defects. p value <0.05 was considered statistically significant, and confidence intervals were set at 95%. 279 patients were included into this study. The prevalence rate of OD was 43%, and the average value of ODS score was 6.67. 17% patients presented straining at stool, 33% presented incomplete emptying, 13% presented digitations, and 12% required laxatives or enema. The prevalence rate of true rectocele was 23%. Defecation symptoms were significantly correlated with age, levator-ani hiatus, levator-ani muscle injury and true rectocele. Logistic regression showed that true rectocele and increased levator-ani hiatus were independent risk factors of OD. True rectocele was significantly correlated with straining at stool, digitation, incomplete emptying and requirement of laxatives or enema.In POP patients, the prevalence rate of true rectocele and OD was 23% and 43%, respectively. True rectocele was related to oD. tLUS was a valuable approach in anatomical evaluation of pop. Pelvic organ prolapse (POP) is a type of disease caused by the defect of pelvic floor supporting structure, which leads the pelvic organ to leave its own anatomical position. Constipation is a common symptom in POP patients 1. Previous study has shown that 50% of people with chronic constipation have symptoms of obstructed defecation (OD). OD is characterized by straining at stool, incomplete emptying, digitation (finger extrusion in perineum or vagina to assist defecation) and the requirement of laxatives or enema 2. Therefore, there should be certain prevalence rate of OD in POP patients. Regarding the relationship between anatomical defects of POP and obstructed constipation, recent studies have suggested that OD is significantly correlated with posterior pelvic anatomic abnormalities, such as true rectocele, enteric hernia and intussusceptions 3. Some scholars think that posterior vaginal prolapse (PVP) indicates rectocele, while a recent study has shown that true rectocele is only observed in 39% women 4. The prevalence of true rectocele and its effect on POP patients remain largely unexplored. Although video-defecography is considered to be the "gold standard" for the diagnosis of rectocele, a number of previous studies have confirmed that translabial ultrasound (TLUS) has good consistency and is in good agreement with video-defecography 5-8. In the present study, we selected TL...