The prevalence of abnormal leukocyte count, and its predisposing factors, in patients with sickle cell disease in Saudi Arabia

Ahmed, Anwar; Ali, Yosra; Alsuliman, Ahmed; Albagshi, Jafar M.; Salamah, Majid Al; Elsayid, Mohieldin; Alanazi, Wala R; Ahmed, Rayan A; McClish, Donna K.; Al-Jahdali, Hamdan

doi:10.2147/jbm.s148463

Cited by 20 publications

(20 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 26 Several reports indicate that high WBC counts usually observed in SCD population are mainly due to bacterial infections. 8 , 9 , 27 – 29 The current study, however, did not establish a relationship between bacterial infections and WBC counts among the SCD patients. The results from this study revealed that people with the severer form of the SCD have higher counts of WBC and PLTs.…”

Section: Discussioncontrasting

confidence: 61%

“…A previous report indicated that SCD patients have elevated white blood cell (WBC) counts, 6 , 8 , 9 activated granulocytes, monocytes, and endothelial cells, enhanced expression of endothelial cell adhesion molecules, elevated cytokine levels and elevated acute-phase reactants. 7 Moreover, another study has reported that the use of drugs, such as Hydroxyurea, lowers WBC count and thus improves the clinical outcome of SCD patients.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hematological parameters in Ghanaian sickle cell disease patients

Antwi‐Boasiako¹,

Ekem²,

Abdul-Rahman³

et al. 2018

JBM

View full text Add to dashboard Cite

BackgroundEffective treatment and management of sickle cell disease (SCD) has been a challenge in Africa over the years. Hematological parameters are very useful profiles in the effective management of the disease. However, there is scarcity of studies on the hematological parameters of SCD in Ghana. This study aimed at determining hematological parameters among SCD patients with vaso-occlusion, those in the steady state as well as healthy controls at a teaching hospital in Ghana.MethodologyThis was a cross-sectional study involving a total of 628 subjects, including 148 HbAA controls, 208 HbSS patients in steady state, 82 HbSC patients in steady state, 156 HbSS patients in vaso-occlusive crises (VOC), and 34 HbSC patients in VOC. Venous blood sample was collected from all study participants. A full blood count was done within 2 hours of collection, and hemoglobin (Hb) concentration, packed cell volume, red blood cell (RBC) concentration, mean corpuscular Hb, mean cell volume, mean corpuscular Hb concentration, and white blood cells (WBC) and platelet (PLT) counts were recorded.ResultsWBC and PLT counts were significantly higher in both female and male patients with SCD, compared with their healthy counterparts (P<0.05). The level of WBC was, however, significantly higher in patients with HbSS VOC among the SCD patients (P<0.001). Levels of Hb, RBC, and hematocrit were significantly higher in the controls (P<0.001). There was no significant difference in mean cell Hb among male patients with SCD (P=0.274) and female patients with SCD (P=0.5410).ConclusionThe SCD patients had lower Hb and RBC than the controls; however, higher PLT and WBC are noted in various status of SCD, possibly reflecting spleen effect in these patients. Further studies are needed to confirm these findings.

show abstract

Section: Discussioncontrasting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

Hematological parameters in Ghanaian sickle cell disease patients

Antwi‐Boasiako¹,

Ekem²,

Abdul-Rahman³

et al. 2018

JBM

View full text Add to dashboard Cite

show abstract

“…Neutrophils have a central role in VOCs through their interactions with both the erythrocytes and the endothelium; neutrophil count and their activity have been consistently correlated with disease severity. [2, 3]…”

Section: Introductionmentioning

confidence: 99%

Neutrophils remain detrimentally active in hydroxyurea-treated patients with sickle cell disease

et al. 2019

View full text Add to dashboard Cite

Neutrophilia is a feature of sickle cell disease (SCD) that has been consistently correlated with clinical severity and has been shown to remain highly activated even at steady state. In addition to induction of fetal hemoglobin (HbF), hydroxyurea (HU) leads to reduction in neutrophil count and their adhesion properties, which contributes to the clinical efficacy of HU in SCD. Although HU reduces the frequency and severity of acute vaso-occlusive crises (VOCs) and chest syndrome, HU therapy does not abolish these acute clinical events. In this study we investigated whether neutrophils in SCD patients whilst on HU therapy retained features of detrimental pro-inflammatory activity. Freshly isolated neutrophils from SCD patients on HU therapy at steady state and from ethnic-matched healthy controls were evaluated ex vivo for their degranulation response and production of neutrophil extracellular traps (NETs). Unstimulated SCD patient neutrophils already produced NETs within 30 minutes, compared to none for healthy neutrophils, and by 4 hours, these neutrophils produced significantly more NETs than the control neutrophils (P = 0.0079**). Higher numbers of neutrophils from SCD patients also showed higher degree of degranulation-related intracellular features compared to healthy neutrophils, including rough-textured cellular membranes (P = 0.03*), double-positivity for F-Actin and CD63 (P = 0.02*) and re-located CD63 within cytoplasm more efficiently than their healthy counterparts (P = 0.02*). The neutrophils from SCD donors released more myeloperoxidase (P = 0.02*) in the absence of any trigger. Our data showed that neutrophils from patients with SCD at steady state remained active during hydroxyurea treatment and are likely to be able to contribute to the SCD pro-inflammatory environment.

show abstract

“…Elevated white blood cell count has been associated with many complications in SCD [29]. Previous reports revealed a possible association between elevated white blood cell count and bacterial infection or vaso-occlusive crisis in sickle cell patients and elevated white blood cell count was associated with admission and frequent emergency department visits [29][30][31].…”

Section: Discussionmentioning

confidence: 99%

Patterns and patient factors associated with loss to follow-up in the Muhimbili Sickle Cell Cohort, Tanzania

Masamu

Sangeda

Kandoga

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Monitoring patient's clinical attendance is a crucial means of improving retention in care and treatment programmes. Sickle cell patients' outcomes are improved by participation in comprehensive care programmes, but these benefits cannot be achieved when patients are lost from clinical care. In this study, patients are defined as loss to follow-up when they did not attend clinic for more than nine months. Precise information on the retention rate and characteristics of those who are not following their clinic appointments is needed to enable the implementation of interventions that will be effective in increasing the retention to care. Method: This was a retrospective study involving sickle cell patients registered in the Muhimbili Sickle Cohort in Tanzania. Descriptive and survival analysis techniques both non-parametric methods (Kaplan-Meier estimator and Log-rank test) and semi-parametric method (Cox’s proportional hazard model), were used. A p-value of 0.05 was considered significant to make an inference from the analysis. Results: A total of 5476 patients were registered in the cohort from 2004 to 2016. Of these, 3350 (58.13%) were actively participating in clinics, while 2126 (41.87%) were inactive, of which 1927 (35.19%) were loss to follow-up. We used data from 2004-2014 because between 2015-2016, patients were referred to other government hospitals. From the survival analysis results, pediatric (HR: 14.29,95% CI: 11.0071-18.5768, p <0.001) and children between 5-17 years [HR:2.61,95% CI:2.2324 - 3.0705, p< 0.001] patients were more likely to be loss to follow-up than the adult (18 and above years) patients. It was found that patients with above averages for hematocrit (HR: 2.38, 95% CI: 1.0076 - 1.0404, p=0.0039) or mean cell volume (HR: 4.28, (95% CI: 1.0260 - 1.0598, p<0.001) were more likely to be loss to follow-up than their counterparts. Conclusion: Loss to follow-up is evident in the cohort of patients in long term comprehensive care. It is, therefore, necessary to design interventions that improve patients’ retention. Suggested solutions include refresher training for health care workers and those responsible for patient follow-up on techniques for retaining patients and comprehensive transition programs to prepare patients who are moving from pediatric to adult clinics

show abstract

The prevalence of abnormal leukocyte count, and its predisposing factors, in patients with sickle cell disease in Saudi Arabia

Cited by 20 publications

References 23 publications

Hematological parameters in Ghanaian sickle cell disease patients

Hematological parameters in Ghanaian sickle cell disease patients

Neutrophils remain detrimentally active in hydroxyurea-treated patients with sickle cell disease

Patterns and patient factors associated with loss to follow-up in the Muhimbili Sickle Cell Cohort, Tanzania

Contact Info

Product

Resources

About