The prevalence and pattern of HPV-16 immunostaining in uterine cervical carcinomas in Ethiopian women: a pilot study

Rashed, Mona M.; Bekele, Alemayehu

doi:10.4314/pamj.v8i1.71077

Cited by 5 publications

(1 citation statement)

References 16 publications

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“…The degree of differentiation (keratinization) is an important pathological feature in cervical tumors, indicates the site of infection in the cervical mucosal epithelium (squamo-columnar junction) and susceptible to HPV infection. In this study, majority of SCC cases were classified as non-keratinizing SCC, 68.8% and 26% were keratinizing SCC, this corroborates previous findings from Ethiopia [ 28 ].…”

Section: Discussionsupporting

confidence: 91%

Introduction of p16INK4a as a surrogate biomarker for HPV in women with invasive cervical cancer in Sudan

Sarwath

Bansal

Husain

et al. 2017

Infect Agents Cancer

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BackgroundCervical cancer is the fourth most common cancer in women worldwide with highest incidence reported in Eastern Africa in 2012. The primary goal of this study was to study the expression of p16INK4a in squamous cell carcinoma (SCC) of the cervix by immunohistochemistry (IHC) and determine relation with clinico-pathological parameters. This study further explored the correlation of p16INK4a immunostaining with another proliferation marker, Ki-67 and to study if human papillomavirus (HPV) IHC can be used as a marker for detection of virus in high-grade dysplasia.MethodsA total of 90 samples, diagnosed for cervical cancer, were included in the study. Fixed Paraffin Embedded (FFPE) tissue sections were stained with anti-p16INK4a, anti-Ki-67 and anti-HPV antibodies using automated immunohistochemistry platform (ASLink 48-DAKO).ResultsImmunohistochemical protein expression of p16INK4a positivity was found to be highest in SCC (92.2%, n = 71) than other HPV tumors (76.9%, n = 10). The majority of cases (97.4%) were p16INK4a positive in the age group 41–60 years. In addition, a statistically significant difference between p16INK4a and HPV was observed among total cervical tumor cases and SCC cases.ConclusionsAs expected staining of invasive cervical cancer with anti-HPV showed rare positivity because HPV heralds active infection in dysplastic lesions and not of frank cervical carcinoma. In contrast, anti-p16INK4a IHC results showed positive correlation in SCC and other cervical tumors.Electronic supplementary materialThe online version of this article (doi:10.1186/s13027-017-0159-0) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 91%

Introduction of p16INK4a as a surrogate biomarker for HPV in women with invasive cervical cancer in Sudan

Sarwath

Bansal

Husain

et al. 2017

Infect Agents Cancer

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Human Papilloma Virus Early Proteins E6 (HPV16/18-E6) and the Cell Cycle Marker P16 (INK4a) are Useful Prognostic Markers in Uterine Cervical Carcinomas in Qassim Region- Saudi Arabia

Omran

Al-Sheeha

2014

Pathol. Oncol. Res.

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Cervical cancer is a common and an important public health problem for adult women in developing countries. In contrast, cervical cancer incidence is low in Saudi Arabia. High-risk types of human papilloma viruses (HPV16 and HPV18) are the most significant risk factors for cervical cancer. HPV16/18-E6 oncoprotein is associated with HPV etiology, viral persistence and epithelial transformation. Cell cycle protein p16 INK4a (p16) plays an important role in the pathophysiology of cervical carcinomas. The aims of this study were to investigate the expression of HPV16/18-E6 and p16 in uterine cervical carcinomas in Qassim Region--Saudi Arabia, and to relate the results to the established clinicopathological prognostic parameters (age of the patient, educational level, birth control methods, number of pregnancy, smoking status, degree of histological differentiation, clinical stage, and lymph node metastasis) The study included 40 specimens of uterine cervical squamous cell carcinomas diagnosed and confirmed by biopsy. Histopathological classification of cervical tumors cases was performed according to the International Federation of Gynecology and Obstetrics (FIGO). Immunohistochemical analysis for HPV16/18-E6 and p16 were carried out on formalin-fixed paraffin-embedded sections of cervical tissues using avidin-biotin peroxidase method. There was a significant statistical correlation between HPV16/18-E6 expression in cervical carcinoma and nationality, smoking status and size of the tumor. HPV16/18-E6 oncoprotein expression in normal lymphocytes and endothelial cells in the tumor tissues and the adjacent normal cervical tissues suggest the possibility that HPV infection might spread to other organs through blood circulation. P16 expression has been correlated with high grade, stage of cervical SCC and HPV16/18-E6 expression. The current study supports the critical function of p16 and HPV16/18-E6 as specific markers for cervical carcinoma. However the potential for usage of p16 and HPV16/18-E6 as prognostic markers will require detailed follow data for a larger group of patients.

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Caractéristiques épidémiologiques et histopathologiques de 1280 cancers du col utérin à Kinshasa

Katumbayi¹,

Muyulu²,

Zakayi³

et al. 2022

Gynécologie Obstétrique Fertilité & Sénologie

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The prevalence and pattern of HPV-16 immunostaining in uterine cervical carcinomas in Ethiopian women: a pilot study

Cited by 5 publications

References 16 publications

Introduction of p16INK4a as a surrogate biomarker for HPV in women with invasive cervical cancer in Sudan

Introduction of p16INK4a as a surrogate biomarker for HPV in women with invasive cervical cancer in Sudan

Human Papilloma Virus Early Proteins E6 (HPV16/18-E6) and the Cell Cycle Marker P16 (INK4a) are Useful Prognostic Markers in Uterine Cervical Carcinomas in Qassim Region- Saudi Arabia

Caractéristiques épidémiologiques et histopathologiques de 1280 cancers du col utérin à Kinshasa

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