The prevalence and distribution of Anaplasma phagocytophilum genotypes in Ixodes ricinus nymphs collected from farm- and woodland sites in Ireland

Flattery, Amie; McKiernan, Fiona; Browne, John A.; Gray, Jeremy; Zaid, Taher; O’Connor, Jack; Zintl, Annetta

doi:10.1016/j.ttbdis.2022.101928

Cited by 4 publications

(2 citation statements)

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“…Canine infection has been recorded following blood transfusion, and transmission to the fetus via placenta has been reported in cattle [14], while transmission from mother to child immediately before or after birth has been documented in humans [15]. Cases of canine, ovine, equine, bovine, and human anaplasmosis have been reported globally and infection of questing ticks, ruminants and wildlife has been reported in Europe over the past decade [16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Anaplasma phagocytophilum strain analysis in cattle from Great Britain

Apaa

McFadzean

Gandy³

et al. 2023

Preprint

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Anaplasma phagocytophilum (A. phagocytophilum) is the aetiological organism of tick-borne fever in cattle and sheep, and granulocytic anaplasmosis in dogs and human. Reports of livestock and wildlife infection and associated zoonotic risk of human infection with A. phagocytophilum have been reported across the world. A previous study has reported two strains, also called ecotypes, in ticks collected from across England and Wales. This study examined A. phagocytophilum strains present in livestock and wildlife in Great Britain (GBR), with a particular focus on cattle. Clinical submissions (EDTA blood) from livestock were received by APHA for tick-borne disease testing. Of these 24 (cattle: n = 21; sheep: n = 3) were confirmed to be infected with A. phagocytophilum using a PCR targeting the msp2 gene. Further submissions from roe deer (n = 2), red deer (n = 2) and Ixodes ricinus ticks (n = 22) were also shown to be infected with A. phagocytophilum. Subsequent analysis using a nested PCR targeting the groEL gene and sequencing, confirmed the presence of ecotype I in cattle, sheep, red deer and Ixodes ricinus, and ecotype II in roe deer and I. ricinus ticks removed from the deer carcass. Despite the presence of two ecotypes, widely distributed in ticks from England and Wales, only ecotype I was detected in cattle in this study.

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Section: Introductionmentioning

confidence: 99%

Anaplasma phagocytophilum strain analysis in cattle from Great Britain

Apaa

McFadzean

Gandy³

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Transmission from mother to child immediately before or after birth has been documented in humans [ 14 ]; transmission to the foetus via placenta has also been reported in cattle [ 15 ]. Cases of canine, ovine, equine, bovine and human anaplasmosis have been reported globally and the infection of questing ticks, ruminants and wildlife has been reported in Europe over the past decade [ 16 , 17 , 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Anaplasma phagocytophilum Ecotype Analysis in Cattle from Great Britain

Apaa

McFadzean

Gandy³

et al. 2023

Pathogens

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Anaplasma phagocytophilum (A. phagocytophilum) is the aetiological agent of tick-borne fever in cattle and sheep, and granulocytic anaplasmosis in human and dogs. Livestock, companion animal and human infections with A. phagocytophilum have been reported globally. Across England and Wales, two isolates (called ecotypes) have been reported in ticks. This study examined A. phagocytophilum isolates present in livestock and wildlife in Great Britain (GB), with a particular focus on cattle. Clinical submissions (EDTA blood) from cattle (n = 21) and sheep (n = 3) were received by APHA for tick-borne disease testing and the animals were confirmed to be infected with A. phagocytophilum using a PCR targeting the Msp2 gene. Further submissions from roe deer (n = 2), red deer (n = 2) and Ixodes ricinus ticks (n = 22) were also shown to be infected with A. phagocytophilum. Subsequent analysis using a nested PCR targeting the groEL gene and sequencing confirmed the presence of ecotype I in cattle, sheep, red deer and Ixodes ricinus, and ecotype II in roe deer and I. ricinus removed from deer carcasses. Despite the presence of two ecotypes, widely distributed in ticks from England and Wales, only ecotype I was detected in cattle in this study.

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