The Prestige oil spill: A laboratory study about the toxicity of the water-soluble fraction of the fuel oil

Stress Biomarkers in Juvenile Senegal Sole, Solea senegalensis, Exposed to the Water-Accommodated Fraction of the “Prestige” Fuel Oil

“…These levels are in line with the exposure doses from our study (19.3, 9.6 and 0.19 lg/L for the 1:5,000, 1:10,000 and 1:50,000, respectively). Similarly to our observations, PAH levels obtained from the water soluble fraction (WSF) of the same ''Prestige'' crude of about 70 lg/L (Navas et al 2006) caused no toxicity associated to an RTG-2 cell line, the algae Chlorela vulgaris or the crustacean Daphnia magna. A 1:500 dilution of the WSF of the crude was the lowest dilution to produce a significant EROD induction in the RTG-2 cell line (Navas et al 2006).…”

“…Similarly to our observations, PAH levels obtained from the water soluble fraction (WSF) of the same ''Prestige'' crude of about 70 lg/L (Navas et al 2006) caused no toxicity associated to an RTG-2 cell line, the algae Chlorela vulgaris or the crustacean Daphnia magna. A 1:500 dilution of the WSF of the crude was the lowest dilution to produce a significant EROD induction in the RTG-2 cell line (Navas et al 2006). No citotoxicity, using a bivalve embryogenesis bioassay, was associated to exposures to polluted ''Prestige'' sediment either (Franco Table 1 Total wet weight (TW), protein yield (PY) and biomarkers of stress in Solea senegalensis 70 dph exposed to different dilutions of the Water Accommodated Fraction (WAF) of the Prestige crude oil .…”

Bioaccumulation and biochemical responses in mussels exposed to the water-accommodated fraction of the <i>Prestige</i> fuel oil

“…This lack of response in our study is supported by the fact that much higher levels of encapsulated Ph, Fl and BaP, than those found in our mussels, were required to exert an effect on CAT and NADPH reductase in M. edulis (Krishnakumar et al, 1997) or for water-borne BaP to cause CYP1A induction and oxidative damage in M. galloprovincialis (Canova et al, 1998). In fact, dilutions lower than 1:500 of the WAF of the Prestige oil were necessary to induce the MFO dependent EROD activity in exposed RTG-2 cells (Navas et al, 2006).…”

“…These components have been reported to have, at sublethal environmentally realistic concentrations, a narcotic effect on invertebrates (Barata et al, 2005), exhorting certain genotoxicity in M. edulis (Hamountene et al, 2002) but a negligible toxic effect on fish (Seruto et al, 2005). Moreover, no cytotoxic effects were observed in RTG-2 cells, no effects on the growth of Chlorella vulgaris nor acute or reproductive effects on Daphnia magna exposed to the water accommodated fraction (WAF) of the Prestige oil (Navas et al, 2006).…”

Severe abnormalities in the reproductive organs of mice caused by chemical substances contained in heavy oil

“…the marine ecosystem, including effects on mussels, et al, 2004;Alonso-Alvarez et al, 2007). Some chemical components that a fraction of the heavy oil is soluble in water may affect the organisms (Navas et al, 2006). oil on marine organisms, few studies have investigated its effects on the health of mammals and humans.…”