The Preservation of Yeast Cultures by Lyophilization

Atkin, Lawrence; Moses, W.W.; Gray, Philip P.

doi:10.1128/jb.57.6.575-578.1949

Cited by 29 publications

(6 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When comparing the yeast survival rates obtained from different preservation methods, the survival of freeze‐dried yeast is lower than that obtained from freezing, l ‐drying or drying (Atkin et al . ; Miyamoto‐Shinohara et al . ; Prakash et al .…”

Section: Discussionmentioning

confidence: 99%

Influence of freezing temperatures prior to freeze-drying on viability of yeasts and lactic acid bacteria isolated from wine

et al. 2017

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Influence of freezing temperatures prior to freeze-drying on viability of yeasts and lactic acid bacteria isolated from wine

et al. 2017

View full text Add to dashboard Cite

show abstract

“…1960). However, not all strains survive the process (Atkin et al. 1949; Smith and Onions 1983) and, among those surviving, viability was progressively reduced over time (Abadias et al.…”

Section: Discussionmentioning

confidence: 99%

Drying of Epicoccum nigrum conidia for obtaining a shelf-stable biological product against brown rot disease

Larena¹,

Cal²,

Liñán³

et al. 2003

J Appl Microbiol

View full text Add to dashboard Cite

Aims: The effects of freeze-drying, spray-drying and fluidized bed-drying on survival of Epicoccum nigrum conidia were compared. Methods and Results: Viability of E. nigrum conidia (estimated by measuring its germination) was 100% after fluidized bed-drying and freeze-drying, but it was determined that skimmed milk must be added in the case of freeze-drying conidia. Addition of other protectants (Tween-20, peptone, sucrose, glucose, starch and peptone + starch) to skimmed milk before freeze-drying did not improve the conidial viability which was obtained with skimmed milk alone. Glycerol had a negative effect on the lyophilization of E. nigrum conidia. Epicoccum nigrum conidia freeze-dried with skimmed milk, or fluidized bed-dried alone maintained an initial viability for 30 and 90 days, respectively, for storage at room temperature. Epicoccum nigrum conidial viability after spray-drying was lower than 10%. Conclusions: The best method to dry E. nigrum conidia was fluidized bed-drying. Conidia without protectants dried by this method had 100% viability and survived for 90 days at room temperature. Significance and Impact of Study: This paper deals with methods for the potential formulation of a biocontrol agent which is being tested for eventual commercialization.

show abstract

“…A few investigators, including Fennell et al (49) and Wickerham and Andreason (207), recommend lyophilization as the method of choice for preservation of certain fungi and yeast, especially C. albicans. There have been few reports of physiological changes in yeast preserved by lyophilization (10,74) and of low viability after storage. As pointed out by Morris (122), there is an unfortunate dearth of information on the use of this technique.…”

Section: Preservation Of Culturesmentioning

confidence: 99%