The Presence of the Virulence Island Containing the Usp Gene in Uropathogenic Escherichia Coli Is Associated With Urinary Tract Infection in an Experimental Mouse Model

Yamamoto, Shingo; Nakano, Masayuki; Terai, Akito; Yuri, Kazuyo; Nakata, Katsuhisa; Nair, G. Balakrish; Kurazono, Hisao; Ogawa, Osamu

doi:10.1016/s0022-5347(01)69897-5

Cited by 48 publications

(40 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a series of studies, we reported usp as a new urovirulence factor that was more frequently found in UPEC strains associated with different symptoms in humans (3). Using a pyelonephritis mouse model, we also demonstrated that the presence of the virulence island containing usp in UPEC was associated with infection (9).…”

Section: Discussionmentioning

confidence: 74%

“…The PI contains usp that encodes a 346-amino acid protein, which we designated as uropathogenic specific protein (Usp), and three small open reading frames (orfU1-3). Further, we demonstrated that Usp significantly enhanced the infectivity of E. coli whereas the three small proteins downstream of usp failed to exhibit this effect in mouse urinary tract infection model (9). The structural and sequence diversity of the PI of UPEC which encodes the Usp protein 798 H. KURAZONO ET AL Fig.…”

mentioning

confidence: 87%

See 1 more Smart Citation

Distribution of the usp Gene in Uropathogenic Escherichia coli Isolated from Companion Animals and Correlation with Serotypes and Size‐Variations of the Pathogenicity Island

Kurazono

Nakano

Yamamoto

et al. 2003

Microbiology and Immunology

Self Cite

View full text Add to dashboard Cite

Escherichia coli is a major causative agent of uncomplicated urinary tract infection (UTI) and these strains are designated as uropathogenic E. coli (UPEC). UTI occurs not only among humans, but is also observed in companion animals such as dogs and cats (7). Although the etiological bacterial agents of UTI are diverse, E. coli is the chief causative pathogen in dogs and cats suffering from UTI of bacterial origin. UTI caused by E. coli has been associated with urogenital disease such as cystitis, nephritis, metritis and prostatitis in dogs and cats (7). Seven virulence factors (VFs), including type 1 pilus (pil), pilus associated with pyelonephritis (pap), S fimbriae (sfa), afimbrial adhesin I (afaI), hemolysin (hly), aerobactin (aer) and cytotoxic necrotizing factor 1 (cnfI) have been documented to play an important role in causing human UTI (2). Several serotypes such as O1, O2, O4, O6, O8, O16, O18, O22, O25 and O75 are preferentially associated with UPEC strains and each of the uropathogenic virulence factors is significantly associated with these serotypes (1,6,8). E. coli strains isolated from dogs and cats with UTI have high prevalence of hly and clustered in 5 serotypes including O2, O4, O6, O7 and O18 (7). These serotypes except for O7 are shared with human UPEC strains. Further, E. coli strains isolated from dogs and cats with UTI were similar to E. coli strains isolated from human UTI (4, 10, 11). These findings suggest that similar E. coli strains might be 797 Microbiol. Immunol., 47(10), [797][798][799][800][801][802] 2003 Editor-Communicated Paper Abstract: Uncomplicated urinary tract infection (UTI) caused by uropathogenic Escherichia coli (UPEC) is a serious problem not only among humans but also in companion animals such as dogs and cats. The uropathogenic specific protein gene (usp) is preferentially distributed in UPEC isolates from dogs and cats compared with the distribution of usp in E. coli strains from feces of healthy dogs and cats and this pattern of distribution resembles that observed in human UPEC strains. The UPEC strains from companion animals share common O serotypes like O1, O2, O4, O6, O16, O18, O22, O25 and O75 as those reported for human UPEC. The size variation of the pathogenicity island that includes usp in UPEC from dogs and cats was almost similar to those seen in human UPEC. We propose that dogs and cats are the alternative reservoirs for UPEC strains that are associated with human UTI. Distribution of the usp Gene in Uropathogenic

show abstract

Section: Discussionmentioning

confidence: 74%

mentioning

confidence: 87%

Distribution of the usp Gene in Uropathogenic Escherichia coli Isolated from Companion Animals and Correlation with Serotypes and Size‐Variations of the Pathogenicity Island

Kurazono

Nakano

Yamamoto

et al. 2003

Microbiology and Immunology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Recently, a new urovirulence factor gene, which was more frequently found in UPEC strains, was reported. This gene encoding a protein designated usp (uropathogenic-specific protein) was demonstrated significantly enhancing the infectivity of E. coli in the mouse UTI model 15,18,28 .…”

Section: Introductionmentioning

confidence: 99%

Genotypic characterization of virulence factors in Escherichia coli strains from patients with cystitis

Tiba

Yano

Leite

2008

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

Abbreviations: aerobactin (iucD), afimbrial adhesin (afaB/C), cytolethal distending toxin (cdtB), cytotoxic necrotizing factor type 1 (cnf-1), G adhesin classes of P fimbriae (papG alleles), group II capsule (kpsMTII), α-hemolysin (hly), minor structural subunits of P fimbriae (papE/F), outer membrane protein of P fimbrae (papC), polymerase chain reaction (PCR), S fimbriae (sfaC/D), type 1 fimbriae (fimH), urinary tract infection (UTI), uropathogenic specific protein (usp), uropathogenic Escherichia coli (UPEC), virulence factors (VFs SUMMARYAdhesins (P-fimbriae, S-fimbriae, type 1 fimbriae and afimbrial adhesin), toxins (α-hemolysin and cytotoxic necrotizing factor type 1), iron acquisition systems (aerobactin) and host defense avoidance mechanisms (capsule or lipopolysaccharide) have been shown to be prevalent in Escherichia coli strains associated with urinary tract infections. In this work, 162 Uropathogenic Escherichia coli (UPEC) strains from patients with cystitis were genotypically characterized by polymerase chain reaction (PCR) assay. We developed three multiplex PCR assays for virulence-related genes papC, papE /F, papG alleles, fimH, sfa/foc, afaE, hly, usp, cdtB, iucD, and kpsMTII, all

show abstract

“…E. coli strain Z42 was isolated from a patient suffering from an inflammation of the prostate gland (prostatitis). The DNA element carrying the usp gene was found on the chromosome of a large number of UPEC strains (4) and is significantly more frequently associated with UPEC strains than with faecal E. coli strains from healthy individuals (8). In addition, the contribution to virulence of the Usp protein in uropathogenic E. coli was assessed using an experimental mouse model.…”

Section: Christopher M Thomas Editor-in-chiefmentioning

confidence: 99%

The tadpole effect in Escherichia coli B/r

Grover

2002

Microbiology

View full text Add to dashboard Cite

show abstract

The Presence of the Virulence Island Containing the Usp Gene in Uropathogenic Escherichia Coli Is Associated With Urinary Tract Infection in an Experimental Mouse Model

Abstract: Our results indicate that usp may contribute to the causation of urinary tract infection and may be considered a major virulence determinant of uropathogenic E. coli.

Cited by 48 publications

References 19 publications

Distribution of the usp Gene in Uropathogenic Escherichia coli Isolated from Companion Animals and Correlation with Serotypes and Size‐Variations of the Pathogenicity Island

Distribution of the usp Gene in Uropathogenic Escherichia coli Isolated from Companion Animals and Correlation with Serotypes and Size‐Variations of the Pathogenicity Island

Genotypic characterization of virulence factors in Escherichia coli strains from patients with cystitis

The tadpole effect in Escherichia coli B/r

Contact Info

Product

Resources

About