The presence of Penicillium and Penicillium mycotoxins in food wastes

Rundberget, Thomas; Skaar, Ida; Flåøyen, A.

doi:10.1016/s0168-1605(03)00291-5

Cited by 99 publications

(52 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Roquefortine is a dimethylallyltryptophan-derived metabolite known to be produced in Penicillium roqueforti and several other fungi (Rundberget et al, 2004;Frisvad & Filtenborg, 1983;de la Campa et al, 2007), and we have identified this mycotoxin in cultures of different P. chrysogenum strains. The gene cluster responsible for the biosynthesis of this mycotoxin is still being elucidated (A. Gó mez & J. F. Martín, unpublished results).…”

Section: Introductionmentioning

confidence: 82%

The heterotrimeric Gα protein Pga1 regulates biosynthesis of penicillin, chrysogenin and roquefortine in Penicillium chrysogenum

et al. 2008

View full text Add to dashboard Cite

We have studied the role of the pga1 gene of Penicillium chrysogenum, encoding the alpha subunit of a heterotrimeric G protein, in secondary metabolite production. The dominant activating pga1 G42R mutation caused an increase in the production of the three secondary metabolites penicillin, the yellow pigment chrysogenin and the mycotoxin roquefortine, whereas the dominant inactivating pga1 G203R allele and the deletion of the pga1 gene resulted in a decrease of the amount of produced penicillin and roquefortine. Chrysogenin is produced in solid medium as a yellow pigment, and its biosynthesis is clearly enhanced by the presence of the dominant activating pga1 G42R allele. Roquefortine is produced associated with mycelium during the first 3 days in submerged cultures, and is released to the medium afterwards; dominant activating and inactivating pga1 mutations result in upregulation and downregulation of roquefortine biosynthesis recpectively. Pga1 regulates penicillin biosynthesis by controlling expression of the penicillin biosynthetic genes; the three genes pcbAB, pcbC and penDE showed elevated transcript levels in transformants expressing the pga1 G42R allele, whereas in transformants with the inactivating pga1 G203R allele and in the pga1-deleted mutant their transcript levels were lower than those in the parental strains. Increase of intracellular cAMP levels had no effect on penicillin production. In summary, the dominant activating pga1 G42R allele upregulates the biosynthesis of three secondary metabolites in Penicillium chrysogenum to a different extent.

show abstract

Section: Introductionmentioning

confidence: 82%

The heterotrimeric Gα protein Pga1 regulates biosynthesis of penicillin, chrysogenin and roquefortine in Penicillium chrysogenum

et al. 2008

View full text Add to dashboard Cite

show abstract

“…Roquefortine C (Fig. 1), containing tryptophan and histidine moieties, was isolated first from Penicillium roqueforti (9) and identified later in many Penicillium strains (10,11), including different strains of P. roqueforti used for cheese production (9,11). Roquefortine C is also the precursor of further prenylated indole alkaloids such as meleagrine (12), oxaline (12,13), and glandicolines (12).…”

Section: From the Philipps-universität Marburg Institut Für Pharmazementioning

confidence: 99%

Acetylaszonalenin Biosynthesis in Neosartorya fischeri

Yin

Grundmann

Cheng

et al. 2009

Journal of Biological Chemistry

154

129

View full text Add to dashboard Cite

Based on the structural information of acetylaszonalenin isolated from Neosartorya fischeri, a putative biosynthetic gene cluster was identified in the genome sequence of this fungus by genomic mining. This cluster consists of three genes coding for a putative non-ribosomal peptide synthetase (AnaPS), a prenyltransferase (AnaPT), and an acetyltransferase (AnaAT). The coding sequences of anaPT and anaAT were cloned in pQE70 and pQE60, respectively, and overexpressed in Escherichia coli. The soluble His 6 fusion proteins were purified to near homogeneity and characterized biochemically. The structures of the enzymatic products were elucidated by NMR and mass spectroscopy analysis. AnaPT was found to catalyze the reverse prenylation of (

show abstract

“…Furthermore, A. niger can produce malformins, naphtho-r-pyrones, nigerazine B, nigragillin and ochratoxin A, while roquefortine C, PR toxin and mycophenolic acid are produced by P. roqueforti (Sahin and Korukluoglu, 2000; Varga et al, 2000;Taniwaki et al, 2001;Erdogan et al, 2003;Blumenthal, 2004;Rundberget et al, 2004). Thus, the presence and growth of these fungi in food products and feed threaten human and animal health.…”

Section: Introductionmentioning

confidence: 99%

The fungicidal efficacy of various commercial disinfectants used in the food industry

2006

View full text Add to dashboard Cite

The antifungal effects of eight commercial disinfectants namely alcohol, peracetic acid, iodophors, aldehydes, quaternary amine compounds (QAC, a, b and c), and a chlorine-based agent were assessed at different concentrations. The time taken for these disinfectants to kill different microorganisms was used to assess their efficacy. The microorganisms tested were six yeasts, Saccharomyces cerevisiae, Saccharomyces uvarum, Kloeckera apiculata, Candida oleophila, Metschnikowia fructicola, Schizosaccharomyces pombe, and two moulds, Aspergillus niger (5 strains) and Penicillium roqueforti (5 strains). The disinfectants QAC (a) and QAC (c) were the most effective against all the microorganisms tested. The chlorine-based disinfectant worked most efficiently against the moulds at all concentrations used (0.5, 1.0, 1.5 and 2.0%). Peracetic acid and alcohol based disinfectants were most effective against the yeasts than mould. Tested yeasts were more resistant to the aldehyde and iodophors base disinfectants than the others.

show abstract

The presence of Penicillium and Penicillium mycotoxins in food wastes

Cited by 99 publications

References 17 publications

The heterotrimeric Gα protein Pga1 regulates biosynthesis of penicillin, chrysogenin and roquefortine in Penicillium chrysogenum

The heterotrimeric Gα protein Pga1 regulates biosynthesis of penicillin, chrysogenin and roquefortine in Penicillium chrysogenum

Acetylaszonalenin Biosynthesis in Neosartorya fischeri

The fungicidal efficacy of various commercial disinfectants used in the food industry

Contact Info

Product

Resources

About