The presence of mycoviral infection attenuates the growth and pathogenicity of the phytopathogenic fungus Botrytis cinerea collected from strawberry fields in Pakistan

Ahmed, Aqeel; Khan, Haris Ahmed; Jamal, Atif; Baig, Danish Ilyas; Fatma, Tehsin; Virk, Nasar; Bhatti, Muhammad Faraz

doi:10.1007/s10658-023-02689-w

Cited by 2 publications

(1 citation statement)

References 36 publications

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“…During a mycoviral screening survey in 2017-18, the fungal strain Kst14a (Botrytis cinerea) was collected and identi ed from an affected strawberry plant leaf [21]. To isolate pure cultures, leaves were surface sterilized with 1% sodium hypochlorite solution before being washed with autoclaved sterile distilled water.…”

Section: Screening Fungal Strains For Mycovirusesmentioning

confidence: 99%

Characterization of two novel fusariviruses co-infecting a single isolate of phytopathogenic fungus Botrytis cinerea

Ahmed,

Khan,

Jamal

et al. 2024

Virus Genes

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A wide diversity of mycoviruses have been reported from Botrytis species, some with the potential to supress the pathogenic abilities of this fungus. Considering their importance, this study was devised to nd potential hypovirulence associated mycoviruses from in Botrytis cinerea strains isolated from Pakistani strawberry elds. Here we report complete genome characterization of two novel fusariviruses co-infecting single isolate of phytopathogenic fungus Botrytis cinerea. Viral genomes were sequenced via deep sequencing using total RNA fractions from isolate Kst14. Identi ed viruses were tentatively named Botrytis cinerea fusarivirus 8 (BcFV8) and Botrytis cinerea fusarivirus 9 (BcFV9). Both viruses had a single segmented (ssRNA) genome having a size of 6,424 and 8,370 nucleotides encoding two discontinuous open reading frames (ORFs). ORF1 of both viruses encodes for a poly-protein having a conserved domain of RNA dependent RNA polymerase (RdRP) and a helicase domain (Hel) which function in RNA replication, while ORF2 encodes a hypothetical protein with an unknown function, respectively. Phylogenetic analysis indicated that BcFV8 made clade with the genus alphafusariviruses and BcFV9 made fall in the genus betafusarivirus in the family Fusariviridae. To our knowledge, this is the rst report of two fusariviruses identi ed in B. cinerea isolates from Pakistan. Both viruses successfully transfected to a compatible strain of Botrytis cinerea (Mst11). A comparison of virus free and infected isogenic line showed that the presence of these viruses was causing hypovirulence in infected strains. Virus infected strains also had a small lesion size while testing the pathogenicity via apple assay.

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Section: Screening Fungal Strains For Mycovirusesmentioning

confidence: 99%

Characterization of two novel fusariviruses co-infecting a single isolate of phytopathogenic fungus Botrytis cinerea

Ahmed,

Khan,

Jamal

et al. 2024

Virus Genes

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View full text Add to dashboard Cite

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Pre-harvest treatments: A different insight into preservation of strawberries

Xu,

Wang,

Chai

et al. 2024

JBR

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Strawberry is one of the most favored consumed fresh fruits worldwide. However, the major constraint limiting future sustainable production and sales of strawberries is post-harvest decay. Thus, taking a deep look into the effective preservation measures including pre- and post-harvest techniques to retain freshness and inhibit the pathological spoilage of strawberries in the supply chain is of great significance and necessity. At present, relying solely on post-harvest technology is not enough to fix the aim of strawberry fruit preservation and longer shelf life. On the contrary, pre-harvest treatments could be extend shelf life of strawberry fruit and adapt to the diverse post-harvest environments. This review give the latest pre-harvest treatments, their effects on post-harvest quality and storability of strawberries, and develop the expectation of strawberries pre-harvest methods applicated in the future.

show abstract

The presence of mycoviral infection attenuates the growth and pathogenicity of the phytopathogenic fungus Botrytis cinerea collected from strawberry fields in Pakistan

Cited by 2 publications

References 36 publications

Characterization of two novel fusariviruses co-infecting a single isolate of phytopathogenic fungus Botrytis cinerea

Characterization of two novel fusariviruses co-infecting a single isolate of phytopathogenic fungus Botrytis cinerea

Pre-harvest treatments: A different insight into preservation of strawberries

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