The presence of microcystins and other cyanobacterial bioactive peptides in aquatic fauna collected from Greek freshwaters

Gkelis, Spyros; Lanaras, T.; Sivonen, Kaarina

doi:10.1016/j.aquatox.2006.02.001

Cited by 93 publications

(84 citation statements)

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“…However, few studies have simultaneously evaluated multiple fish organs, such as the gut, kidney and heart, which may also accumulate MCs and affect the health of aquatic animals. MCs accumulate in the phytoplanktivorous silver carp (Hypophthalmichthys molitrix), herbivorous white bream (Parabramis pekinensis), omnivorous common carp (Cyprinus carpio),crucian carp (Carassius auratus) and carnivorous species, including the predatory carps (Culter ilishaeformisand Culter erythropterus), the yellow catfish (Pseudobagrus fulvidraco), Osbeck's grenadier anchovy (Coilia ectenes) and the salangid icefish (Neosalanx taihuensis); however, it is not currently known which fish species accumulate the highest amounts of MCs in their tissues (Gkelis et al, 2006;Xie et al, 2005;Zhang et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Bioaccumulation of microcystins (MCs) in four fish species from Lake Taihu, China: Assessment of risks to humans

Jia

Luo

Lü

et al. 2014

Science of The Total Environment

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Microcystins (MCs) are the toxic products of harmful algal blooms and they accumulate in fish. The accumulation of MCs in fish living in different trophic levels from different parts of Lake Taihu was determined. This information was then used to evaluate the risks posed by the MCs in fish to human health. The concentrations of three MCs, MC-LR, MC-YR and MC-RR, were quantified in the following four fish species: silver carp (Hypophthalmichthys molitrix), bighead carp (Aristichthys nobilis), crucian carp (Carassius auratus) and common carp (Cyprinus carpio), using high performance liquid chromatography interfaced with tandem (triple quadrupole) mass spectrometry. The mean concentrations of MCs in the muscle, the kidney, the intestinal wall and the heart were significantly different among the four fishes except in the liver. C. carpio contained the highest mean concentration of MCs in the muscle (31.7 ± 12.1 ng/g, dry mass (dm)), whereas C. auratus had the highest mean concentrations of MCs in the liver (45.4 ± 44.5 ng/g, dm), kidney (114 ± 51.1 ng/g, dm), intestinal wall (2.04 × 10 3 ± 4.43 × 10 3 ng/g, dm) and heart (59.5 ± 26.7 ng/g, dm). The mean concentration of MCs in the intestinal walls of the fish species was significantly higher than in other organs (p b 0.01). The fish from Meiliang Bay had significantly higher concentrations of MCs than those from the centre, west or south banks of the lake (p b 0.01). The body lengths and masses of the fish were negatively correlated with the concentrations of MCs in the kidney (p b 0.05) and heart (p b 0.01). The average daily intake (ADI) of MCs in the muscle of all fishes exceeded the provisional tolerable daily intake (TDI) set by World Health Organization. The estimated daily intakes of MCs in 55.6% of the muscle samples exceeded the TDI. The MCs in the tissues of the fish from Lake Taihu pose potential risks to the health of humans who consume these four fish species.

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Section: Introductionmentioning

confidence: 99%

Bioaccumulation of microcystins (MCs) in four fish species from Lake Taihu, China: Assessment of risks to humans

Jia

Luo

Lü

et al. 2014

Science of The Total Environment

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“…X and Z represent variable L-amino acids in positions 2 and 4, respectively (Carmichael, 1992). Similar to MCs, anabaenopeptins (APN) have been reported to inhibit protein phosphatase 1 (APN A and B; Gkelis et al, 2006) as well as proteases such as carboxypeptidase A (APN G and H, Itou et al, 1999). APNs contain six amino acids: five of them form a cyclic structure with a sixth amino acid that is attached.…”

Section: Introductionmentioning

confidence: 99%

Variation in peptide net production and growth among strains of the toxic cyanobacteriumPlanktothrixspp.

Kosol

Schmidt

Kurmayer

2009

European Journal of Phycology

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Cyanobacteria frequently form mass developments in surface waters. Populations consist of strains that differ in the production of bioactive peptides, e.g. microcystins (MC) inhibiting protein phosphatases 1 and 2A and anabaenopeptins (APN) inhibiting carboxypeptidases. Forty-nine strains (18 green-pigmented (phycocyanin-rich) P. agardhii strains and 31 redpigmented (phycoerythrin-rich) P. rubescens strains) of the filamentous cyanobacterium Planktothrix (Anagnostidis et Komare´k) were analysed for their MC and APN net production rates. These rates were compared with (i) the pigmentation, (ii) the proportion of extra-and intracellular peptide concentrations and (iii) the cellular growth rates under standardized laboratory conditions. Excluding the strains lacking MC and APN, the MC and APN contents varied up to 14-fold and 12-fold, each. The variation in minimum and maximum peptide content (0.32-4.51 mg MC mg À1 dry weight; 0.85-10.32 mg APN mg À1 dry weight) exceeded the variation found for chlorophyll a (4.8-16.9 mg mg À1 dry weight). The extracellular proportions of MC (0-62%) and APN (0-58%) varied among strains, however, on average, proportions of extracellular MC and APN were low (MC: 8.8 AE (1 SE) 1.9%, APN: 8.4 AE 1.8%). Among all strains cellular growth rates showed a 5-fold variation (0.07-0.33 doublings of dry weight.day À1) and were found independent of the pigmentation and the peptide net production rate. It is concluded that the MC and APN net production rate is not causally related to the cell-division cycle and the synthesis of highest amounts of MC and APN does not constrain cell division.Key words: cyanobacteria, cyanotoxins, freshwater phytoplankton, harmful algal bloom, growth rate, high performance liquid chromatography, Microcystis, water reservoir IntroductionBecause of their frequent occurrence in fresh water, toxin-producing cyanobacteria are considered to be a general threat to drinking water quality (Codd et al., 1999; WHO, 2004). Many of the studies dealing with toxin production have focused on microcystins (MC), cyclic heptapeptides inhibiting eukaryotic proteinphosphatase 1 and 2A. MCs are synthesized via non-ribosomal peptide synthetases (NRPS) and polyketide synthases (PKS) assembled into large multifunctional proteins encoded by the mcy gene cluster (Tillett et al., 2000). , where D-MeAsp is the non-proteinogenic amino acid D-erythro--iso-aspartic acid (methyl aspartate), Mdha is N-methyl-dehydroalanine and Adda is a -amino acid with a C 10 -chain: (2S, 3S, 8S, 9S)-3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid. X and Z represent variable L-amino acids in positions 2 and 4, respectively (Carmichael, 1992). Similar to MCs, anabaenopeptins (APN) have been reported to inhibit protein phosphatase 1 (APN A and B; Gkelis et al., 2006) as well as proteases such as carboxypeptidase A (APN G and H, Itou et al., 1999). APNs contain six amino acids: five of them form a cyclic structure with a sixth amino acid that is attached. The pentapeptide moiety has the common struct...

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“…Alguns autores destacam a presença comprovada das microcistinas em diferentes animais aquáticos, como peixes (DE-BLOIS et al, 2008), zooplânctons (FERRÃO-FILHO et al, 2002), caramujos (ZHANG et al, 2009a;2009b), gastró-podes (ZHANG et al, 2007) e bivalves (DIONISIO PIRES et al, 2004;GKELIS et al, 2006).…”

Section: Introductionunclassified

Acumulação de microcistinas no mexilhão dourado Limnoperna fortunei e riscos para a biota aquática

Minillo

Casali

Isique

et al. 2016

Rev. Bras. Ciênc. Ambient. (Online)

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Endereço para correspondência:Alessandro Minillo -Universidade Estadual do Mato Grosso do SulRodovia Dourados/Ithaum, km 12, 7804-9970 -Dourados (MS), Brasil -E-mail: alminillo@yahoo.com.br RESUMOAs cianobactérias representam um componente natural da comunidade fitoplanctônica, mas estão frequentemente associadas com águas eutrofizadas. Os rios do estado de São Paulo estão em processo contínuo de eutrofização, o que favorece frequentes florações de cianobactérias, prejuízos à qualidade da água para o consumo humano e riscos à biota aquática. O mexilhão dourado (Limnoperna fortunei) é uma espécie exótica invasora que pode ser vetor na transferência de toxinas de cianobactérias. O objetivo deste estudo foi avaliar a ocorrência de florações de cianobactérias no rio São José dos Dourados, suas toxinas produzidas e a possibilidade de ficotoxinas bioacumularem em Limnoperna fortunei. Foram detectadas, nas amostras, a dominância de cianobactérias tóxicas e cianotoxinas (microcistinas). Os resultados indicaram que L. fortunei acumularam microcistinas. As florações de cianobactérias nas águas do rio estudado indicaram prejuízo ao ambiente associado à eutrofização e aos riscos da presença das cianotoxinas à biota aquática.Palavras-chave: bioacumulação; cianobactéria; ficotoxinas; espécies exóticas; molusco; eutrofização. ABSTRACTCyanobacterias are natural components of the phytoplankton community, but they are often associated with eutrophic waters. Rivers in the state of São Paulo are in a continuous process of eutrophication, which favors the frequent cyanobacteria blooms in these environments, compromises the quality of the water for human consumption and promotes risks to the aquatic biota. The golden mussel (Limnoperna fortune) is an invasive alien species that can be a vector in the transfer of cyanobacterial toxins. The aim of this study was to evaluate the cyanobacteria blooms occurrence in São José dos Dourados River, their cyanotoxins and the possibility of phycotoxins bioaccumulating in the Limnoperna fortunei. Toxic cyanobacterias and cyanotoxins (microcystins) were detected in the samples. The results indicated that L. fortunei accumulated microcystins. The cyanobacterial blooms in the water of the studied river indicated a damage to the environment associated with eutrophication and risks of cyanotoxins presence to the aquatic biota.

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The presence of microcystins and other cyanobacterial bioactive peptides in aquatic fauna collected from Greek freshwaters

Cited by 93 publications

References 47 publications

Bioaccumulation of microcystins (MCs) in four fish species from Lake Taihu, China: Assessment of risks to humans

Bioaccumulation of microcystins (MCs) in four fish species from Lake Taihu, China: Assessment of risks to humans

Variation in peptide net production and growth among strains of the toxic cyanobacteriumPlanktothrixspp.

Acumulação de microcistinas no mexilhão dourado Limnoperna fortunei e riscos para a biota aquática

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