The Presence of Kinesin Superfamily Motor Proteins KIFC1 and KIF17 in Normal and Pathological Human Placenta

Satı, Leyla; Seval-Celik, Yasemin; Unek, Gozde; Korgun, Emin Türkay; Demir, Ramazan

doi:10.1016/j.placenta.2009.07.005

Cited by 14 publications

(11 citation statements)

References 36 publications

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“…The three dimensional analysis of P. clarkii KIFC1 protein shows a 350-residue C-terminal globular motor domain that is conserved in the kinesin family [23], N-terminal species specific cargo binding domain that is slightly longer than that of other crustaceans, and a helical neck region that links these two domains. KIFC1 has been shown to participate in chromosomal and spindle movement during mitosis and meiosis, and transport membranous organelles and macromolecules fundamental to cellular functions [24]. It was found that kifc1 mRNA was expressed in varieties of tissues in P. clarkii including testis, heart, green gland, muscle and gill, implying a wide function in P. clarkii , although there existed a difference in kifc1 levels among tissues, with higher expressions in testis, heart, green gland while a relatively lower expressions in muscle and gill.…”

Section: Discussionmentioning

confidence: 99%

KIFC1 is essential for acrosome formation and nuclear shaping during spermiogenesis in the lobster Procambarus clarkii

Yang

2017

Oncotarget

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In order to study the function of kinesin-14 motor protein KIFC1 during spermatogenesis of Procambarus clarkii, the full length of kifc1 was cloned from testes cDNA using Rapid-Amplification of cDNA Ends (RACE). The deduced KIFC1 protein sequence showed the highest similarity between Procambarus clarkii and Eriocheir senensis (similarity rate as 64%). According to the results of in situ hybridization (ISH), the kifc1 mRNA was gathered in the acrosome location above nucleus in the mid- and late-stage spermatids. Immunofluorescence results were partly consistent with the ISH in middle spermatids, while in the late spermatids the KIFC1 was distributed around the nucleus which had large deformation and formed four to six nuclear arms. In the mature sperm, KIFC1 and microtubules were distributed around the sperm, playing a role in maintaining the sperm morphology and normal function. Overexpression of P. clarkii kifc1 in GC1 cells for 24 hours resulted in disorganization of microtubules which changed the cell morphology from circular and spherical into fusiform. In addition, the overexpression also resulted in triple centrosomes during mitosis which eventually led to cell apoptosis. RNAi experiments showed that decreased KIFC1 protein levels resulted in total inhibition of spermatogenesis, with only mature sperm found in the RNAi-testis, implying an indispensable role of KIFC1 during P. clarkii spermiogenesis.

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Section: Discussionmentioning

confidence: 99%

KIFC1 is essential for acrosome formation and nuclear shaping during spermiogenesis in the lobster Procambarus clarkii

Yang

2017

Oncotarget

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“…KIF17 has been localized to the vascular endothelium of early, normal and pathological term human placental villi, where vasculogenesis and angiogenesis occur [90]. Its presence in hematopoietic and Hofbauer cells of early but not term placentas raises the possibility that KIF17 may be involved in the development of hematopoietic cells and in placental vascular formation.…”

Section: Diverse Functions Of Kif17mentioning

confidence: 99%

The kinesin superfamily protein KIF17: one protein with many functions

Wong‐Riley

Besharse²

2012

BioMolecular Concepts

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Kinesins are ATP-dependent molecular motors that carry cargos along microtubules, generally in an anterograde direction. They are classified into 14 distinct families with varying structural and functional characteristics. KIF17 is a member of the kinesin-2 family that is plus end-directed. It is a homodimer with a pair of head motor domains that bind microtubules, a coiled-coil stalk, and a tail domain that binds cargos. In neurons, KIF17 transports N-methyl-D-aspartate receptor NR2B subunit, kainate receptor GluR5, and potassium Kv4.2 channels from cell bodies exclusively to dendrites. These cargos are necessary for synaptic transmission, learning, memory, and other functions. KIF17’s interaction with NXF2 enables the transport of mRNA bidirectionally in dendrites. KIF17 or its homolog OSM-3 also mediates intraflagellar transport of cargos to the distal tips of flagella or cilia, thereby aiding in ciliogenesis. In many invertebrate and vertebrate sensory cells, KIF17 delivers cargos that contribute to chemosensory perception and signal transduction. In vertebrate photoreceptors, KIF17 is necessary for outer segment development and disc morphogenesis. In the testis, KIF17 (KIF17b) mediates microtubule-independent delivery of ACT from the nucleus to the cytoplasm and microtubule-dependent transport of Spatial-ε, both are presumably involved in spermatogenesis. KIF17 is also implicated in epithelial polarity and morphogenesis, placental transport and development, and the development of specific brain regions. The transcriptional regulation of KIF17 has recently been found to be mediated by nuclear respiratory factor 1 (NRF-1), which also regulates NR2B as well as energy metabolism in neurons. Dysfunctions of KIF17 are linked to a number of pathologies.

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“…This acts to demonstrate the indispensable roles of such kinesins and kinesin related proteins and particularly suggests the possible pivotal role of KIFC1 for oocyte division and proper embryonic development. Moreover, KIFC1 is also reported to be divergently and periodically expressed during bovine ovarian follicular selection [96] and in the early stage human placenta, in the latter case along with KIF17 [97]. We, therefore, suspect a role of KIFC1 in gestation.…”

Section: Potential Risks Of Targeting Kifc1-related Systemsmentioning

confidence: 89%

KIFC1: a promising chemotherapy target for cancer treatment?

Xiao

Yang

2016

Oncotarget

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The kinesin motor KIFC1 has been suggested as a potential chemotherapy target due to its critical role in clustering of the multiple centrosomes found in cancer cells. In this regard, KIFC1 seems to be non-essential in normal somatic cells which usually possess only two centrosomes. Moreover, KIFC1 is also found to initiatively drive tumor malignancy and metastasis by stabilizing a certain degree of genetic instability, delaying cell cycle and protecting cancer cell surviving signals. However, that KIFC1 also plays roles in other specific cell types complicates the question of whether it is a promising chemotherapy target for cancer treatment. For example, KIFC1 is found functionally significant in vesicular and organelle trafficking, spermiogenesis, oocyte development, embryo gestation and double-strand DNA transportation. In this review we summarize a recent collection of information so as to provide a generalized picture of ideas and mechanisms against and in favor of KIFC1 as a chemotherapy target. And we also drew the conclusion that KIFC1 is a promising chemotherapy target for some types of cancers, because the side-effects of inhibiting KIFC1 mentioned in this review are theoretically easy to avoid, while KIFC1 is functionally indispensable during mitosis and malignancy of multi-centrosome cancer cells. Further investigations of how KIFC1 is regulated throughout the mitosis in cancer cells are needed for the understanding of the pathways where KIFC1 is involved and for further exploitation of indirect KIFC1 inhibitors.

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The Presence of Kinesin Superfamily Motor Proteins KIFC1 and KIF17 in Normal and Pathological Human Placenta

Cited by 14 publications

References 36 publications

KIFC1 is essential for acrosome formation and nuclear shaping during spermiogenesis in the lobster Procambarus clarkii

KIFC1 is essential for acrosome formation and nuclear shaping during spermiogenesis in the lobster Procambarus clarkii

The kinesin superfamily protein KIF17: one protein with many functions

KIFC1: a promising chemotherapy target for cancer treatment?

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