The presence of JC virus in gastric carcinomas correlates with patient's age, intestinal histological type and aberrant methylation of tumor suppressor genes

Ksiâa, Feryel; Ziadi, Sonia; Mokni, Moncef; Korbi, S.; Trimèche, Mounir

doi:10.1038/modpathol.2009.184

Cited by 27 publications

(30 citation statements)

References 40 publications

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“…In this study only two samples (1 GC and 1 GA) were positive for JCPyV LTag sequences. Our result reveals a very low frequency of JCPyV LTag sequences in GC and other gastroduodenal diseases, which is inconsistent with a number of other reports [15,23,36]. One possible explanation for this inconsistency is the high percentage of FFPE samples in our GC group (67.9%); the detection rate of JCPyV DNA sequences could be significantly reduced in FFPE samples [15,38].…”

Section: Discussioncontrasting

confidence: 57%

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No Evidence for an Association between JC Polyomavirus Infection and Gastroduodenal Diseases

Cherati

Yahyapour

Ranaee

et al. 2018

Gastrointest Tumors

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Background: Helicobacter pylori (HP) infection is one of the hypothesized infectious etiologies of gastric cancer (GC) and other gastroduodenal diseases. It was suggested that other infectious agents, including oncogenic viruses, may increase the risk of gastroduodenal diseases. A number of reports regarding JC polyomavirus (JCPyV) have shown that JCPyV could be implicated in colorectal cancer and gastrointestinal carcinogenesis. Objective: The current investigation aimed to investigate whether JCPyV could have any association with the pathogenesis of gastroduodenal diseases either alone or together with HP. Methods: A total of 237 fresh or formalin-fixed and paraffin-embedded gastroduodenal samples were examined by quantitative real-time polymerase chain reaction targeting the JCPyV large tumor antigen (LTag) oncogene, and viral load was determined as viral copy number/cell. Results: In total, 2 out of 237 samples (0.8%) were positive for JCPyV LTag DNA. One positive sample derived from diffuse-type gastric adenocarcinoma (6.8 × 10^–3 copies/cell) and other JCPyV-positive sample obtained from a patient with gastritis (2.5 × 10^–3 copies/cell) were recorded. Both JCPyV-positive samples were negative for HP infection. Conclusion: This study suggests no association between JCPyV infection and GC or other gastroduodenal diseases. The very low frequency of JCPyV LTag sequences in GC is an important aspect that weakens the hypothesis of the pathogenic role of JCPyV in gastric tumor induction.

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Section: Discussioncontrasting

confidence: 57%

“…Another plausible explanation could be geographic and ethnic variations in JCPyV distribution. The JCPyV LTag sequence was detected in 26.0% of Tunisian GC patients [36]. In addition, 57.0% of Korean [23] and 86.3% of Japanese [15] GC patients harbored the JCPyV LTag sequence in their neoplastic tissues.…”

Section: Discussionmentioning

confidence: 99%

No Evidence for an Association between JC Polyomavirus Infection and Gastroduodenal Diseases

Cherati

Yahyapour

Ranaee

et al. 2018

Gastrointest Tumors

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“…Importantly, Bofill-Mas, et al showed that viral particles isolated from sewage remained intact after treatment at a pH of 1 for 30 minutes, indicating that ingestion of contaminated water or food may be sufficient for JCV infection of the gastrointestinal tract [20]. Many studies since, have demonstrated the presence of JCV genomic sequences and the expression of T-Antigen in tissues from gastrointestinal tumors, including esophageal carcinoma [11], gastric carcinoma [12], [21], [22], sporadic adenomatous polyps [23], and colorectal adenocarcinomas [10], [24]–[30].…”

Section: Introductionmentioning

confidence: 99%

Activation of c-Myc and Cyclin D1 by JCV T-Antigen and β-Catenin in Colon Cancer

et al. 2014

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During the last decade, mounting evidence has implicated the human neurotropic virus JC virus in the pathology of colon cancer. However, the mechanisms of JC virus-mediated oncogenesis are still not fully determined. One candidate to mediate these effects is the viral early transcriptional product T-Antigen, which has the ability to inactivate cell cycle regulatory proteins such as p53. In medulloblastomas, T-Antigen has been shown to bind the Wnt signaling pathway protein β-catenin; however, the effects of this interaction on downstream cell cycle regulatory proteins remain unknown. In light of these observations, we investigated the association of T-Antigen and nuclear β-catenin in colon cancer cases and the effects of this complex in the activation of the transcription and cell cycle regulators c-Myc and Cyclin D1 in vitro. Gene amplification demonstrated the presence of viral sequences in 82.4% of cases and we detected expression of T-Antigen in 64.6% of cases by immunohistochemistry. Further, we found that T-Antigen and β-catenin co-localized in the nuclei of tumor cells and we confirmed the physical binding between these two proteins in vitro. The nuclear presence of T-Antigen and β-catenin resulted in the significant enhancement of TCF-dependent promoter activity and activation of the β-catenin downstream targets, c-Myc and Cyclin D1. These observations provide further evidence for a role of JCV T-Antigen in the dysregulation of the Wnt signaling pathway and in the pathogenesis of colon cancer.

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“…More recently, JCV was also found in non-neural cancers, such as gastric [9] and lung cancers [10]. JCV infection was first reported as a potential risk factor for colorectal cancer (CRC) in a work by Laghi et al [11], which found that 96% of CRC tissues were positive for JCV DNA sequences.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of JC Virus in Chinese Patients with Colorectal Cancer

et al. 2012

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BackgroundJCV is a DNA polyomavirus very well adapted to humans. Although JCV DNA has been detected in colorectal cancers (CRC), the association between JCV and CRC remains controversial. In China, the presence of JCV infection in CRC patients has not been reported. Here, we investigated JCV infection and viral DNA load in Chinese CRC patients and to determine whether the JCV DNA in peripheral blood (PB) can be used as a diagnostic marker for JCV-related CRC.Methodology/Principal FindingsTumor tissues, non-cancerous tumor-adjacent tissues and PB samples were collected from 137 CRC patients. In addition, 80 normal colorectal tissue samples from patients without CRC and PB samples from 100 healthy volunteers were also harvested as controls. JCV DNA was detected by nested PCR and glass slide-based dot blotting. Viral DNA load of positive samples were determined by quantitative real-time PCR. JCV DNA was detected in 40.9% (56/137) of CRC tissues at a viral load of 49.1 to 10.3×104 copies/µg DNA. Thirty-four (24.5%) non-cancerous colorectal tissues (192.9 to 4.4×103 copies/µg DNA) and 25 (18.2%) PB samples (81.3 to 4.9×103 copies/µg DNA) from CRC patients were positive for JCV. Tumor tissues had higher levels of JCV than non-cancerous tissues (P = 0.003) or PB samples (P<0.001). No correlation between the presence of JCV and demographic or medical characteristics was observed. The JCV prevalence in PB samples was significantly associated with the JCV status in tissue samples (P<0.001). Eleven (13.8%) normal colorectal tissues and seven (7.0%) PB samples from healthy donors were positive for JCV.Conclusions/SignificanceJCV infection is frequently present in colorectal tumor tissues of CRC patients. Although the association between JCV presence in PB samples and JCV status in tissue samples was identified in this study, whether PB JCV detection can serve as a marker for JCV status of CRC requires further study.

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The presence of JC virus in gastric carcinomas correlates with patient's age, intestinal histological type and aberrant methylation of tumor suppressor genes

Cited by 27 publications

References 40 publications

No Evidence for an Association between JC Polyomavirus Infection and Gastroduodenal Diseases

No Evidence for an Association between JC Polyomavirus Infection and Gastroduodenal Diseases

Activation of c-Myc and Cyclin D1 by JCV T-Antigen and β-Catenin in Colon Cancer

Prevalence of JC Virus in Chinese Patients with Colorectal Cancer

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