The presence of F cells with a fetal phenotype in adults with hemoglobinopathies limits the utility of flow cytometry for quantitation of fetomaternal hemorrhage

Othman, Jad; Orellana, Daniel; Chen, Lin Selina; Russell, Megan R.; Khoo, Teh‐Liane

doi:10.1002/cyto.b.21598

Cited by 6 publications

(5 citation statements)

References 9 publications

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“…It is more expensive but more accurate and automated. With this method, it is possible to precisely detect and characterize a low number of cells (e.g., fetal RBCs) present in large cell populations [ 30 , 31 , 32 ]. The evaluation of FMH using flow cytometry requires minimal blood (only approximately 20 µL for fetal hemoglobin/carbonic anhydrase staining).…”

Section: Discussionmentioning

confidence: 99%

Is Feto-Maternal Transfusion after Cesarean Delivery Different in Singleton and Twin Pregnancy?

Stachurska-Skrodzka,

Mielecki,

Fijałkowska

et al. 2024

JCM

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Background: The aim of the study was to investigate if feto-maternal transfusion was related to the size of the fetal-maternal interface, and, therefore, was larger in twin pregnancy in comparison with singleton pregnancy. Methods: Blood samples from women with singleton (n = 11), and monochorionic (n = 11) and dichorionic (n = 13) twin gestations were tested. Flow cytometry tests with hemoglobin F, glycophorin A, and hemoglobin F and carbonic anhydrase simultaneous staining were used to detect fetal red blood cells and maternal F cells. Results: In all cases, the volume of feto-maternal transfusion was estimated to be low. The highest rate of fetal red blood cells in the maternal circulation was observed in the blood of women with dichorionic twin gestations both before and after delivery. An increase in fetal red blood cells was observed after cesarean section in singletons and twins. The median rate of maternal F cells was 2.23% in singleton, 2.1% in monochorionic and 3.95% in dichorionic pregnancy. Conclusions: Feto-maternal transfusion during pregnancy may be related to the multiplicity and chorionicity of pregnancy.

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Section: Discussionmentioning

confidence: 99%

Is Feto-Maternal Transfusion after Cesarean Delivery Different in Singleton and Twin Pregnancy?

Stachurska-Skrodzka,

Mielecki,

Fijałkowska

et al. 2024

JCM

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“…Both methods have similar results in detecting different classes of hemoglobin, including the HbF fraction, which opens up the possibility of HPLC use in FMH detection. However, we did not use HPLC for this purpose [10,11].…”

Section: Discussionmentioning

confidence: 99%

Fetomaternal Hemorrhage: Can We Use Hemoglobin Electrophoresis as a Diagnostic Tool?

Stanić

Stanišić

Fureš

et al. 2019

Z Geburtshilfe Neonatol

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Fetomaternal hemorrhage (FMH) is usually detected by either the Kleihauer-Betke (KB) test or by 2 cytometry, the latter of which represents the gold standard of FMH diagnosis today. But what do we do when neither method is available? We present two cases of suspected FMH due to their characteristic signs and symptoms that were ultimately confirmed by hemoglobin electrophoresis (HE).

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“…The following clinical conditions may result in an increased level of HbF due to elevated levels of adult F cells, and should not be confused with a fetomaternal hemorrhage: severe anemia, hereditary persistence of HbF, and sickle cell disease in crisis or under therapy with hydroxyurea, butyrate, or other drugs that elevate F cells (Corcoran, Murphy, Donnelly, & Ainle, 2014, Othman, Orellana, Chen, Russell, & Khoo, 2018.…”

Section: Critical Parameters and Troubleshootingmentioning

confidence: 99%

Enumeration of Fetal Red Blood Cells, Hemoglobin‐Specific RBC Cells, and F Reticulocytes in Human Blood

Davis

2019

CP Cytometry

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Recent advances in analytical cytometry have improved diagnostic tools for the study of erythropoiesis in anemic patients and resolution of differential diagnosis in diseases of the erythron. This article presents three applications of red blood cell (RBC) analysis-quantitation of fetal red cells, F-cell enumeration, and F-reticulocyte analysis-which improve diagnostic precision, sensitivity, and specificity, and provide better laboratory indicators of therapeutic efficacy in a variety of hematologic and obstetric disorders. Such advances also include the measurement and quantitation of RBC hemoglobins and their relative ribonucleic acid levels. These advances not only promise to improve diagnostic accuracy and laboratory precision over techniques such as the traditional manual reticulocyte counting method and the Kleihauer-Betke stain method for evaluating fetomaternal hemorrhage (FMH), but also serve as tools for newer assays of anemia diagnosis and improved clinical outcomes. In addition to the primary methods, supporting techniques for preparing spiked controls, automating data analysis, setting up a fetal hemoglobin acquisition protocol, and assaying reticulocytes using thiazole orange are also presented. C 2019 by John Wiley & Sons, Inc. Keywords: F cells r F reticulocytes r fetal hemoglobin r fetal red cells r fetomaternal hemorrhage r flow cytometry r hemoglobin levels r red blood cells How to cite this article: Davis, B. H. (2019). Enumeration of fetal red blood cells, hemoglobin-specific RBC cells, and F reticulocytes in human blood.

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The presence of F cells with a fetal phenotype in adults with hemoglobinopathies limits the utility of flow cytometry for quantitation of fetomaternal hemorrhage

Cited by 6 publications

References 9 publications

Is Feto-Maternal Transfusion after Cesarean Delivery Different in Singleton and Twin Pregnancy?

Is Feto-Maternal Transfusion after Cesarean Delivery Different in Singleton and Twin Pregnancy?

Fetomaternal Hemorrhage: Can We Use Hemoglobin Electrophoresis as a Diagnostic Tool?

Enumeration of Fetal Red Blood Cells, Hemoglobin‐Specific RBC Cells, and F Reticulocytes in Human Blood

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