The Presence of Bradykinin-Like Polypeptides, Kinin-Releasing and Destroying Activity in Brain

Hori, Seiki

doi:10.2170/jjphysiol.18.772

Cited by 54 publications

(24 citation statements)

References 4 publications

(4 reference statements)

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“…The findings obtained on the isolated guinea pig ileum are in accordance with the results reported by others (Hori, 1968;Camargo et al , 1973;Roberts, 1980). Since Camargo and co-workers (1973) found that fragments of bradykinin induce a negligible contraction of the guinea pig ileum, it is evident that the effects measured are really due to bradykinin and not to a product of catabolism.…”

Section: Discussionsupporting

confidence: 91%

“…Components of an intracerebral bradykinin system have been described previously. Kininogen-and kininogenase-like activity have been found in the brains of rabbits, rats, cattle, and bull-frogs (Hori, 1968;Shikimi et aI. , 1973), although measurable amounts of kininogen could not be detected in the cortex of cats (Baethmann et aI.…”

Section: Lund Swedenmentioning

confidence: 99%

“…, 1981). Kinin-like activity and immunoreactive bradykinin localized in the neuronal cells have been observed, primarily in the hypothalamus (Hori, 1968;Pela et aI., 1975). However, bradykinin-containing fibres also exist in superficial layers of cerebral cortex (Correa et aI.…”

Section: Lund Swedenmentioning

confidence: 99%

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Effects of Bradykinin on Pial Arteries and Arterioles in vitro and in situ

Wahl¹,

Young

Edvinsson

et al. 1983

J Cereb Blood Flow Metab

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Summary: The effect of bradykinin on cerebrovascular resistance vessels was investigated by the use of in vitro and in situ preparations. Bradykinin, in the range of 10-10 to 10-5 M, elicited a concentration-dependent vasodilata tion on both feline and human pial arteries in vitro; the half-maximal response was found to be approximately at 2.8 x 10-7 M and 1.3 x 10-8 M (EC5o), respectively. This dilatatory effect of bradykinin in vitro was found only in arteries pre constricted with prostaglandin F 2a or 5-hydroxytryptamine. In order to determine the effects of bradykinin on the diameter of cat pial arteries in situ, perivascular microapplication was employed. The dose response curves obtained showed vasodilatation; the EC50 and the maximal response (EArn) were 4.4 x 10-7 M

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Section: Discussionsupporting

confidence: 91%

Section: Lund Swedenmentioning

confidence: 99%

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Effects of Bradykinin on Pial Arteries and Arterioles in vitro and in situ

Wahl¹,

Young

Edvinsson

et al. 1983

J Cereb Blood Flow Metab

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“…Most studies demonstrating endogenous bradykinin-like activity in brain have used bioassays, demonstrating the presence in brain extracts of material whose pattern of effects on various smooth muscles is unique to bradykinin and closely related kinins (5)(6)(7)16). These effects include relaxation of the rat duodenum, contraction of the estrous rat uterus and guinea pig ileum, and lowering of blood pressure when administered systemically.…”

Section: Resultsmentioning

confidence: 99%

Bradykinin-like immunoreactive neuronal systems localized histochemically in rat brain.

Corrêa¹,

Innis²,

Uhl³

et al. 1979

Proc. Natl. Acad. Sci. U.S.A.

125

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Bradykinin-like immunoreactive structures were localized in rat brain by the indirect immunofluorescence method. Specificity of staining was demonstrated by: (i) the absence of fluorescence when preimmune serum was used, (ifi) the disappearance of fluorescence when sera were preadsorbed with bradykinin, and (iiM) the presence of identical staining with two different antisera. Immunoreactive neuronal cells are observed only in the hypothalamus, with especially dense clusters overlying the periventricular and dorsomedial nuclei. Fibers and varicose processes are observed in the periaqueductal gray matter, hypothalamus, perirhinal and cingulate cortices, the ventral portion of caudate-putamen, and the lateral septal area. Bradykinin, a nonapeptide first found in mammalian blood (1), is involved in the mediation of several pathophysiological conditions, including inflammation, pain generation, cardiovascular shock, and hypertension (2-4). In addition, a variety of evidence suggests a role for a bradykinin-like system in mammalian brain. Specific bioassay and radioimmunoassay with potent and selective antisera indicate the presence of bradykinin-like activity in extracts of brain tissue (refs. 5-7; unpublished data). Moreover, direct administration of bradykinin in doses from 10 fmol to 1 pmol into the lateral ventricles or restricted areas of the brain produces selective autonomic and behavioral responses (8-10).In the present study, by using two specific antisera to bradykinin, we describe neuronal systems containing bradykinin-like immunoreactivity. MATERIALS AND METHODSImmunofluorescence. Bradykinin-like immunoreactivity was visualized by the indirect immunohistofluorescence method of Coons and coworkers (11), as described earlier (12). Sprague-Dawley male rats (100 g) were injected intracerebroventricularly with 50,ug of colchicine (Sigma) dissolved in 20 ,l of 0.9% NaCl 48 hr before they were killed. Animals were perfused through the heart first for 10 sec with ice-cold normal saline and then for 5-10 min with ice-cold phosphate-buffered 4% depolymerized paraformaldehyde solution. Brains were postfixed for 90 min in this perfusate and then soaked for at least 24 hr in 7% sucrose/0.6 M phosphate buffer, pH 7.4. Brain slabs approximately 0.5 mm thick were rapidly frozen onto cryostat chucks with liquid nitrogen. Sixteen-micrometer sections were cut at -20°C in a Harris cryostat and thaw-mounted onto slides coated with gelatin/chrome alum. Sections were stained at 37°C for 30 min with primary antisera diluted 1:25 with phosphate-buffered saline containing 0.2% Triton X-100. After three 5-min washes with phosphate-buffered saline/0.05% Triton, the sections were exposed for 15 min at 370C to fluorescein-conjugated guinea pig antibody against rabbit IgG (Cappel Laboratories, Cochranville, PA) diluted 1:40 with phosphate-buffered saline/0.1% Triton. The sections were then washed three times (5 min each) in phosphate-buffered saline/0.2% Triton, dipped in H20, and mounted with 0.5 M sodium bicarbonate buff...

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“…Further, the enzymatic inacti vating system of BK at neutral pH was found in tissues in the kidney, lung, heart, spleen, liver and brain (3). Endopeptidases of brain tissue have been isolated and characterized (4)(5)(6)(7)(8)(9). However, few authors have discussed the peptides released from B K. We have already reported that the Ser-Pro containing peptide fragments of BK prolonged pen tobarbital induced sleeping time in mice and the possible importance of Ser-Pro for the sedation phase of behavior induced by BK was suggested (10).…”

mentioning

confidence: 99%

Central action of Bradykinin (II) Separation of Bradykinin degrading enzyme from the rat brain.

et al. 1981

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Abstract-Separation of kininases from the rat brain and identification of the products produced by these enzymes were examined using DEAEcellulose column chromatography and thin-layer chromatography, respec tively. Fraction A (F-A), which was developed with 70 mM NaCl, released Arg-Pro-Pro-Gly-Phe and Ser-Pro-Phe-Arg, fraction B (F-B), with 100 mM NaCl, cleaved the Pro7-Phe8 and Phe8-Arg9 peptide bonds of the brady kinin molecule and fraction C (F-C) with 150 mM NaCl hydrolyzed brady kinin to amino acids. The specific activity of each fraction determined by the bioassay system was 158.7, 51.0 and 14.8 nmole bradykinin/mg protein/ min, respectively. The intensities of their fluorescence on the chromato gram showed visibly that o-phenanthroline effectively inhibited F-A, B and C at a concentration of 0.5 mM. These results suggest that heterogeneous enzyme systems are present in the rat brain and regulate the levels of brady kinin in the brain.

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The Presence of Bradykinin-Like Polypeptides, Kinin-Releasing and Destroying Activity in Brain

Cited by 54 publications

References 4 publications

Effects of Bradykinin on Pial Arteries and Arterioles in vitro and in situ

Effects of Bradykinin on Pial Arteries and Arterioles in vitro and in situ

Bradykinin-like immunoreactive neuronal systems localized histochemically in rat brain.

Central action of Bradykinin (II) Separation of Bradykinin degrading enzyme from the rat brain.

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