The presence of autoantibodies to N-terminus domain of GluR1 subunit of AMPA receptor in the blood serum of patients with epilepsy

Dambinova, Svetlana A.; Izykenova, Galina A.; Буров, С. В.; Grigorenko, Elena; Gromov, S. A.

doi:10.1016/s0022-510x(97)00150-0

Cited by 22 publications

(10 citation statements)

References 19 publications

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“…The levels of antibodies to the glutamate receptor NR2A subunit in the sera of patients with opium narcomania and epilepsy and in donors virtually did not differ. These data are in line with previous reports increased level of antibodies to the glutamate receptor GluR1 subunits in epileptics correlating with the disease duration and severity [11].…”

Section: Resultssupporting

confidence: 93%

Detection of antibodies to opioid and glutamate receptors by latex agglutination and enzyme immunoassay

et al. 2005

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We studied adsorption capacity of 5 latexes to synthetic peptide fragments of mu- and delta-opioid receptors and to GluR1 and NR2A subunits of glutamate receptor. Levels of autoantibodies to opioid receptors in the latex agglutination test and enzyme immunoassay were in good correlation. The level of autoantibodies to opioid receptors measured by these methods was increased in patients with opium narcomania, while the content of autoantibodies to the glutamate receptor subunits was increased in epileptics.

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Section: Resultssupporting

confidence: 93%

Detection of antibodies to opioid and glutamate receptors by latex agglutination and enzyme immunoassay

et al. 2005

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“…The peptide was produced by solidphase synthesis on methylbutyl-hydroacetyl resin, purified by HPLC, immobilized on matrix, and used as an antigen in an ELISA (12 ). Polyclonal antibodies against NR2A/2B peptide were raised by rabbit immunizations, and the IgG fraction of antibodies was purified according to standard techniques by use of chromatography on protein A-agarose and then on NR2A/2B-containing Sepharose 4B (20 ).…”

Section: Antigen Identification and Calibrator Antibodiesmentioning

confidence: 99%

“…Clinical reports have described the effects of stroke on the immune system (10,11 ). Patients with central nervous system disorders have been known to exhibit properties of autoimmunization to products of nerve cell degradation, in particular, damaged neuroreceptors (9,(11)(12)(13).…”

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confidence: 99%

Blood Test Detecting Autoantibodies to N-Methyl-d-aspartate Neuroreceptors for Evaluation of Patients with Transient Ischemic Attack and Stroke

et al. 2003

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Background: Stroke is a multisystemic disorder that includes mechanisms of thrombosis and neurotoxic coupling. Key metabolites of the molecular cascade following biochemical events appear simultaneously in brain tissue, the blood-brain barrier, and brain vessels, activating the immune system and generating autoantibodies (aAbs) to brain-specific antigens. We developed an ELISA blood test to measure aAbs to a subtype of N-methyl-D-aspartate (NMDA) receptors, which are the key markers of neurotoxicity underlying cerebral ischemia. We investigated the diagnostic accuracy of serum aAbs to NR2A/2B, a subtype of NMDA receptors, in assessing transient ischemic attack (TIA) and ischemic stroke (IS) and its ability to distinguish cerebral ischemia from intracerebral hemorrhage (ICH). Methods: Autoantibodies to NR2A/2B were measured in 360 serum samples: 105 from TIA/stroke patients and 255 from controls, including patients with controlled hypertension/atherosclerosis and gender-and agematched healthy individuals. Results: Patients with TIA (n ‫؍‬ 56) and acute IS (n ‫؍‬ 31) had significantly higher NR2A/2B aAb concentrations than controls (P <0.0001). The test sensitivities for TIA and IS were 95% and 97%, respectively, and predictive values were 86% and 91% at a cutoff point of 2.0 g/L. The area under the ROC curve was 0.99. Monitoring NR2A/2B aAbs within 72 h differentiated IS and ICH (P

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“…Recent studies report that increased levels of autoantibodies (aAbs) to several central nervous system (CNS) antigens can be detected in the blood of patients with neuropsychiatric disorders as well as in experimental animal models (Vincent et al 1999;Kowal et al 2006;Zandman-Goddard et al 2007;Dambinova et al 1997Dambinova et al , 1998Knight et al 2007). Increased levels of aAbs to amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) or to N-methyl-D-aspartate (NMDA) receptors have been evidenced in the serum of patients with intractable seizures (Rogers et al 1994;Twyman et al 1995) or brain ischemic stroke (Dambinova et al 2003), respectively.…”

Section: Introductionmentioning

confidence: 99%

Autoantibodies against opioid or glutamate receptors are associated with changes in morphine reward and physical dependence in mice

et al. 2008

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The presence of autoantibodies to N-terminus domain of GluR1 subunit of AMPA receptor in the blood serum of patients with epilepsy

Cited by 22 publications

References 19 publications

Detection of antibodies to opioid and glutamate receptors by latex agglutination and enzyme immunoassay

Detection of antibodies to opioid and glutamate receptors by latex agglutination and enzyme immunoassay

Blood Test Detecting Autoantibodies to N-Methyl-d-aspartate Neuroreceptors for Evaluation of Patients with Transient Ischemic Attack and Stroke

Autoantibodies against opioid or glutamate receptors are associated with changes in morphine reward and physical dependence in mice

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