The presence of ANP in rat peritoneal mast cells

Abstract

Atrial natriuretic peptide (ANP) is an important component of the natriuretic peptide system. A great role in many regulatory systems is played by mast cells. Meanwhile involvement of these cells in ANP activity is poorly studied. In this work, we have shown the presence of ANP in rat peritoneal mast cells. Pure fraction of mast cells was obtained by separation of rat peritoneal cells on a Percoll density gradient. By Western blotting, two ANP-immunoreactive proteins of molecular masses of 2.5 kDa and 16.9 kDa were detected in lysates from these mast cells. Electron microscope immunogold labeling has revealed the presence of ANP-immunoreactive material in storage, secreting and released granules of mast cells. Our findings indicate the rat peritoneal mast cells to contain both ANP prohormone and ANP. These both peptides are located in mast cell secretory granules and released by mechanism of degranulation. It is discussed that many mast cell functions might be due to production of natriuretic peptides by these cells.

Antiallergic effect of KOB03, a polyherbal medicine, on mast cell-mediated allergic responses in ovalbumin-induced allergic rhinitis mouse and human mast cells

“…Hence, this model presents the characteristic pathology of rhinitis such as sneezing, nasal rubbing, congestion, and edema. It is believed that nasal symptoms are mediated not only by histamine but also by prostaglandins, leukotrienes and trypase from activated mast cells, eosinophils and basophils (Martynova et al, 2005;Shinmei et al, 2009). In the present study, KOB03 remarkably inhibited rhinitis symptoms including the clinical scores, the morphological changes of nasal mucosa, and the histamine release in OVA-sensitized mice.…”

Effects of deuterium oxide on cell growth and vesicle speed in RBL-2H3 cells

“…Besides reduction of background fluorescence, the advantage of using TIRF microscopy is selective excitation of vesicles that are within the evanescent field. In mast cells, membrane-bound granules are approximately 300–400 nm in diameter (Martynova et al, 2005). Therefore in the experiments outlined below, vesicles were identified as fluorescent particles of size within the microscope resolution limit.…”

Metabolic engineering of Chinese hamster ovary cells: Towards a bioengineered heparin

“…Peritoneal rat mast cells were isolated from ~6-week old adult rats based on a modification of widely used methods for mast cell isolation and staining (Gustafson and Pihl, 1967; Nemeth and Rohlich, 1980; Martynova et al, 2005). After rats were sacrificed by CO 2 fixation and decapitation, their abdomens were skinned, followed by injection of ~60 ml of sterile phosphate buffered saline (PBS).…”