The presence and absence of magic spot nucleotide modulation in cyanobacteria undergoing nutritional shift‐down

Adams, David G.; Phillips, Derek; Nichols, Josie M.; Carr, N. G.

doi:10.1016/0014-5793(77)80925-3

Cited by 20 publications

(4 citation statements)

References 13 publications

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“…However, there are conflicting results for the correlation between nitrogen starvation and ppGpp accumulation (1,2,11,17,25) and the effect of light (hence, energy) deprivation, which does not seem to be comparable with the nutritional step-down of heterotrophic bacteria (3,17,25). In studies dealing with the light-dependent regulation of gene expression in Synechococcus sp., an obligate autotrophic cyanobacterium, the pleiotropic nature of regulatory responses has been emphasized (9,23).…”

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confidence: 99%

Effects of light deprivation on RNA synthesis, accumulation of guanosine 3'(2')-diphosphate 5'-diphosphate, and protein synthesis in heat-shocked Synechococcus sp. strain PCC 6301, a cyanobacterium

et al. 1987

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The rate of total RNA synthesis, the extent of guanosine 3'(2')-diphosphate 5 '-diphosphate (ppGpp) accumulation, and the pattern of protein synthesis were studied in light-deprived and heat-shocked Synechococcus sp. strain PCC 6301 cells. There was an inverse correlation between the rate of total RNA synthesis and the pool size of ppGpp, except immediately after a temperature shift up, when a parallel increase in the rate of RNA synthesis and accumulation of ppGpp was observed. The inverse correlation between RNA synthesis and ppGpp accumulation was more pronounced when cells were grown in the dark. Heat shock treatment (47°C) had an unexpected effect on ppGpp accumulation; there was a fairly stable level of ppGpp under heat shock conditions, which coincided with a stable steady-state rate of RNA synthesis even in the dark. We found that the pattern of dark-specific proteins was altered in response to heat shock. The transient synthesis of several dark-specific proteins was abolished by an elevated temperature (47C) in the dark; moreover, the main heat shock proteins were synthesized even in the dark. This phenomenon might be of aid in the study of cyanobacterial gene expression.

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confidence: 99%

Effects of light deprivation on RNA synthesis, accumulation of guanosine 3'(2')-diphosphate 5'-diphosphate, and protein synthesis in heat-shocked Synechococcus sp. strain PCC 6301, a cyanobacterium

et al. 1987

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“…mulation of which is elicited by the adverse conditions, is considered to be the major effector of the adjustments (5,6,13,18). In the phototrophic cyanobacteria, however, the universality and exact role of the ppGpp response is still a matter for debate (1,21), although the similarity of the ppGpp responses to starvation in cyanobacteria and E. coli, in a number of cases, is striking (2,17,(26)(27)(28).…”

Section: Resultsmentioning

confidence: 99%

Bacteriophage infection interferes with guanosine 3'-diphosphate-5'-diphosphate accumulation induced by energy and nitrogen starvation in the cyanobacterium Anacystis nidulans

Borbély¹,

Kaki²,

Gulyás³

et al. 1980

J Bacteriol

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Anacystis nidulans accumulates large amounts of guanosine 3'-diphosphate-5'-diphosphate (ppGpp) upon nutritional or energy starvation induced by lightto-dark shift, treatment with carbonylcyanide-m-chlorophenylhydrazone (an uncoupler), or treatment with L-methionine-DL-sulfoximine (an inducer of nitrogen starvation). In contrast to healthy A. nidulans cells, those infected by AS-1 cyanophage do not respond with ppGpp accumulation when starved after about one-third of the complete infection cycle, except, to some extent, under extreme conditions when both nitrogen deprivation and energy deprivation are induced simultaneously (darkening plus L-methionime-DL-sulfoximine treatment). In contrast to cyanophage infection in Anacystis, infection with T4 phage of Escherichia coli CP 78 cells does not affect their accumulation of ppGpp under treatments identical with or similar to those applied in the experiments with Anacystis. This difference in response of phage-infected heterotrophic and photoautotrophic cells to starvation seems to reflect differences in control of nutritional or energy metabolism rather than differences in ability to synthesize ppGpp.

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“…A diverse variety of procaryotes other than E. coli have been examined for the presence of ppGpp and other highly phosphorylated nucleotides. These include: the gram-positive bacteria B. brevis (98), B. subtilis, (21), and B. stearothermophilus (82); the nitrogen-fixing bacteria Rhizobium japonicum and Klebsiella pneumoniae (A. G. Atherly and P. R. Russell, unpublished data); and the photosynthetic bacteria Anacystis nidulans (1,60), Anabaenopsis circularis (84), Anabaena cylindrica (1,84), and Rhodopseudomonas sphaeroides (16). Although the presence of ppGpp has been established in all bacterial species tested, it cannot be concluded that ppGpp is synthesized by the same mechanism as or functions in a similar manner to that in E. coli.…”

Section: Introductionmentioning

confidence: 99%

The search for guanosine tetraphosphate (ppGpp) and other unusual nucleotides in eucaryotes.

Silverman¹,

Atherly²

1979

Microbiological Reviews

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The presence and absence of magic spot nucleotide modulation in cyanobacteria undergoing nutritional shift‐down

Cited by 20 publications

References 13 publications

Effects of light deprivation on RNA synthesis, accumulation of guanosine 3'(2')-diphosphate 5'-diphosphate, and protein synthesis in heat-shocked Synechococcus sp. strain PCC 6301, a cyanobacterium

Effects of light deprivation on RNA synthesis, accumulation of guanosine 3'(2')-diphosphate 5'-diphosphate, and protein synthesis in heat-shocked Synechococcus sp. strain PCC 6301, a cyanobacterium

Bacteriophage infection interferes with guanosine 3'-diphosphate-5'-diphosphate accumulation induced by energy and nitrogen starvation in the cyanobacterium Anacystis nidulans

The search for guanosine tetraphosphate (ppGpp) and other unusual nucleotides in eucaryotes.

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