The preparation of urogastrone

Mongar, J. L.; Rosenoer, Victor M.

doi:10.1113/jphysiol.1962.sp006923

Cited by 12 publications

(12 citation statements)

References 19 publications

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“…The urogastrone, used was prepared from human male urine, as described by Mongar & Rosenoer (1962). Doses are given in terms of weight of a freeze-dried sample.…”

Section: Assay Of Urogastrone 157mentioning

confidence: 99%

The assay of urogastrone

Rosenoer¹,

Schild²

1962

The Journal of Physiology

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Ghosh (1958) reported a method for the assay of inhibitors of acid gastric secretion in the rat based upon the procedure for the continuous recording of gastric secretion described by Ghosh & Schild (1958). This method differed from earlier assay procedures in two main respects: (1) several doses of inhibitor were administered in succession so that each experiment comprised one or more assay blocks; (2) the inhibitory drug was given before the stimulant and was thus used to prevent rather than to counteract the effect of the latter.Whilst this method seemed satisfactory for the assay of atropine-like drugs, Ghosh (1959) found that in assaying the secretory-inhibitory activities of gonadotrophins and related urogastrone-like substances (Ghosh, 1956) it was most economical to administer only one dose of inhibitor to each rat because of the variability in the rate of recovery from inhibition in different rats. The object of the present work has been to modify the procedure for continuous recording of gastric-acid secretion to enable several doses of urogastrone to be given to each animal so as to benefit from the greater precision afforded by multiple doses. A method has been developed in which carbachol is used as the secretory stimulant and a block of four doses of urogastrone is administered to each rat. METHODSThe method for the continuous recording of acid gastric secretion in the urethanized rat maintained at 300C (Ghosh & Schild, 1958) was modified as follows. The operative technique was simplified to avoid any undue handling of the stomach. It was found that instead of opening the stomach simple washing by way of the oesophageal tube with 500 ml. tap water removed all food debris from the secreting portion of the stomach. Some food residues were left in the ruminal portion of the stomach but this did not seem to interfere with the assay.In previous work histamine was used as the secretory stimulant with dilute sodium hydroxide as the perfusing fluid. However, carbachol gave larger and more consistent acid responses and in addition these responses were readily inhibited by urogastrone. We have therefore adopted carbachol as the secretory stimulant, but in order to obtain a linear relation between the recorded pH and the increased acid secreted, we have replaced the dilute sodium hydroxide perfusion fluid with a phosphate-citrate buffer at pH 7x3: 'stock solution', citric acid 24 g+KH2P04 15-6 g made up to 1 1. with distilled water; working solution: 20 ml. stock solution + 8.1 ml. N-NaOH made up to 2 1. with distilled water.

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“…The urogastrone, used was prepared from human male urine, as described by Mongar & Rosenoer (1962). Doses are given in terms of weight of a freeze-dried sample.…”

Section: Assay Of Urogastrone 157mentioning

confidence: 99%

The assay of urogastrone

Rosenoer¹,

Schild²

1962

The Journal of Physiology

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“…This has been fully described elsewhere (Mongar & Rosenoer, 1962). Briefly, the active material was adsorbed on a zeolite column and differentially eluted with a buffered ammonium acetate-ethanol solution.…”

Section: Methodsmentioning

confidence: 99%

“…The development of the assay procedure based upon the continuous recording of acid gastric secretion in the urethanized rat (Rosenoer & Schild, 1962) has permitted a greater degree of precision than has been possible hitherto. The use of a simple one-stage chromatographic technique (Mongar & Rosenoer, 1962) has facilitated the preparation of large quantities of a stable, potent and pyrogen-free urogastrone.…”

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The relation of peptide bonds, disulphide bonds and sulphydryl groups to urogastrone activity

Rosenoer¹

1962

The Journal of Physiology

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“…Methods for the extraction and assay of urogastrone have previously been described by workers from this laboratory (Mongar & Rosenoer, 1962;Rosenoer & Schild, 1962), in which the extraction procedure was based on the absorption of urine on a naturally occurring zeolite, cabunite, followed by desorption. When attempts were made more recently to use cabunite from the same commercial source for the extraction of urogastrone, unexpectedly low yields were obtained.…”

Section: Introductionmentioning

confidence: 99%

Preparation and assay of urogastrone, an inhibitor of gastric acid secretion

Lawrence

Schild

Smith

1971

British J Pharmacology

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Summary1. Urogastrone, an acid gastric secretion inhibitor extracted from human urine, was assayed in the anaesthetized rat by circulating through its stomach a succinic-propionic buffer and recording electrometrically the secretion of acid in response to carbachol, and inhibition of carbachol secretion by urogastrone. 2. Quantitative assay procedures are described requiring the administration of only one or two doses of urogastrone to each rat.3. An extraction procedure for urogastrone is described based on the use of zeocarb, a cationic polystyrene resin.4. The most purified samples contained 40-80 times the amount/milligram which was just detectable by the assay method.

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The preparation of urogastrone

Cited by 12 publications

References 19 publications

The assay of urogastrone

The assay of urogastrone

The relation of peptide bonds, disulphide bonds and sulphydryl groups to urogastrone activity

Preparation and assay of urogastrone, an inhibitor of gastric acid secretion

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