The preparation of sex-chromosome-specific painting probes and construction of sex chromosome DNA library in half-smooth tongue sole (Cynoglossus semilaevis)

Wang, Xubo; Zhang, Quanqi; Ren, Jian; Jiang, Zhanjun; Wang, Changmeng; Zhuang, Wei; Zhai, Teng

doi:10.1016/j.aquaculture.2009.09.020

Cited by 20 publications

(15 citation statements)

References 53 publications

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“…The karyotype of C. semilaevis was observed to be 2n = 42 acrocentric chromosomes, which was in accordance with previous results (Zhou et al, 2005;Wang et al, 2009b). Compared to males, females had a unique and larger W chromosome with no homologous chromosome in the genome ( Figure 1A).…”

Section: Resultssupporting

confidence: 81%

“…Therefore, the distinct heteromorphic W chromosome in C. semilaevis is evolutionarily a neo-sex chromosome, which might evolve during or after speciation. Although several sex-specific probes have been developed from the W chromosome (Wang et al, 2009b;Zhai et al, 2011) of C. semilaevis, the origin and evolution of this neo-chromosome remains unknown. To uncover the genetic basis of this chromosome, as well as the evolutionary status and taxonomy position of this species, further cytogenetic studies must be conducted.…”

Section: Introductionmentioning

confidence: 99%

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Chromosome mapping of 18S rDNA and 5S rDNA by dual-color fluorescence in situ hybridization in the half-smooth tongue sole (Cynoglossus semilaevis)

Jiang

Liu

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Half-smooth tongue sole (Cynoglossus semilaevis) is an important aquaculture flatfish in China. Cytogenetic analysis has revealed that its sex determination system is female heterogametic (ZZ/ZW). The W chromosome is morphologically larger and has been considered evolutionarily younger than any other chromosome in the set. However, the genetic origin and evolution process of this neo-chromosome remains unclear. In this study, 2 tandem arrays of rRNA genes were chosen to address this question. Both the major rDNA (18S rDNA) and the minor rDNA (5S rDNA) were located on the C. semilaevis chromosomes by fluorescence in situ hybridization (FISH). Six 18S rDNA signals were observed on the centromeric regions of 3 pairs of autosomes in both males and females. In females, there was an additional 18S rDNA signal mapping to the telomeric region of the W chromosome long arm. With respect to the 5S rDNA, 12 signals were mapped to the centromeric regions of six pairs of autosomes. Two-color FISH further confirmed that the two pairs of the 5S rDNA signals were correspondingly located at the same positions of the same autosomes as those of the 18S rDNA signals. These results allowed us to speculate about the evolution process of the W chromosome. Chromosome fusions and repetitive sequence accumulations might have occurred in C. semilaevis. The synteny and non-synteny of C. semilaevis 18S rDNA and 5S rDNA might imply the original and evolutionary characteristics of this species. These findings will facilitate studies on karyotype evolution of the order Pleuronectiformes.

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Section: Resultssupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Chromosome mapping of 18S rDNA and 5S rDNA by dual-color fluorescence in situ hybridization in the half-smooth tongue sole (Cynoglossus semilaevis)

Jiang

Liu

et al. 2014

Genet. Mol. Res.

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“…As in other vertebrates, fish sex chromosomes can also be enriched in repetitive DNA sequences [Martins, 2007], as shown by the isolation and mapping of sex-specific repetitive DNAs in this group [Nanda et al, 1990;Devlin et al, 1991;Capriglione et al, 1994;Nakayama et al, 1994;Stein et al, 2001Stein et al, , 2002Harvey et al, 2002;Vicente et al, 2003;Ezaz et al, 2004;Parise-Maltempi et al, 2007;Wang et al, 2009].…”

Section: Accumulation Of Repetitive Dna Sequences In Fish Sex Chromosmentioning

confidence: 99%

“…A clear correlation between sex chromosomes and repetitive DNAs has been pointed out in several studies [Singh et al, 1976;Nanda et al, 1990;Nishioka et al, 1993;Erlandsson et al, 2000;Schmid et al, 2002;Vicente et al, 2003;Steinemann and Steinemann, 2005;Hobza et al, 2006;Marchal et al, 2006;Matsubara et al, 2006;Vicari et al, 2008;Kejnovsky et al, 2009;Wang et al, 2009;, indicating that the differentiation of sex chromosomes is frequently associated with the accumulation of repetitive DNA sequences in these chromosomes. It is well known that repetitive DNAs can also occur and accumulate in the autosomes of several organisms.…”

mentioning

confidence: 99%

Repetitive DNAs and Differentiation of Sex Chromosomes in Neotropical Fishes

Cioffi

Camacho²,

Bertollo³

2010

Cytogenet Genome Res

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The processes working on sex chromosome differentiation are still not completely understood. However, the accumulation of repetitive DNA sequences has been shown to be one of the first steps in the early stages of such differentiation. In addition, regions with suppressed or no recombination have a potential to accumulate these DNA sequences and, for this reason, the absence of recombination between the sex chromosomes favors, by itself, the accumulation of repetitive sequences on these chromosomes during evolution. The diversity of sex-determining mechanisms in fish, alongside with the absence of heteromorphic sex chromosomes in many species, makes this group a useful model to better understand evolutionary processes of sex chromosomes in vertebrates, considering that fish occupy the basal position in the phylogeny of this group. In this review we draw attention to a preferential accumulation and enrichment in repetitive DNAs in sex chromosomes of many neotropical fish species in comparison with autosomes. This phenomenon has been observed between both morphologically differentiated and nascent sex chromosome systems, which highlight the potential role of these sequences in the differentiation of fish sex chromosomes generating differences in morphology and size between them.

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“…The chromosomal organization of the repetitive DNA sequences has provided new insights in understanding genomic evolution in many species, demonstrating that the differentiation of the sex chromosomes is frequently associated with the accumulation of such DNA sequences in the chromosomes [Singh et al, 1976;Nanda et al, 1990;Nishioka et al, 1993;Schmid et al, 2002;Vicente et al, 2003;Steinemann and Steinemann, 2005;Hobza et al, 2006;Marchal et al, 2006;Matsubara et al, 2006;Vicari et al, 2008;Wang et al, 2009;.Unlike higher vertebrates, which are characterized by the origination of sex chromosomes at the base of their phylogeny and by relatively stable sex-determining systems [Dettai et al, 2007], fish exhibit a huge diversity in their sex-determining mechanisms. In addition to the absence of differentiated sex chromosomes in most species, this diversity makes fish a good model for analyzing the differentiation process of sex chromosomes in vertebrates.…”

mentioning

confidence: 99%

Chromosomal Distribution of Repetitive DNA Sequences Highlights the Independent Differentiation of Multiple Sex Chromosomes in Two Closely Related Fish Species

Cioffi

Molina

Moreira‐Filho

et al. 2011

Cytogenet Genome Res

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The arrangement of 6 repetitive DNA sequences in the mitotic and meiotic sex chromosomes of 2 Erythrinidae fish, namely Hoplias malabaricus and Erythrinuserythrinus, both with a multiple X₁X₁X₂X₂/X₁X₂Y sex chromosome system, was analyzed using fluorescence in situ hybridization. The distribution patterns of the repetitive sequences were distinct for each species. While some DNA repeats were species-specific, others were present in the sex chromosomes of both species at different locations. These data, together with the different morphological types of sex chromosomes and the distinct chromosomal rearrangements associated with the formation of the neo-Y chromosomes, support the plasticity of sex chromosome differentiation in the Erythrinidae family. Our present data highlight that the sex chromosomes in fish species may follow diverse differentiation patterns, even in the same type of sex chromosome system present in cofamiliar species.

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The preparation of sex-chromosome-specific painting probes and construction of sex chromosome DNA library in half-smooth tongue sole (Cynoglossus semilaevis)

Cited by 20 publications

References 53 publications

Chromosome mapping of 18S rDNA and 5S rDNA by dual-color fluorescence in situ hybridization in the half-smooth tongue sole (Cynoglossus semilaevis)

Chromosome mapping of 18S rDNA and 5S rDNA by dual-color fluorescence in situ hybridization in the half-smooth tongue sole (Cynoglossus semilaevis)

Repetitive DNAs and Differentiation of Sex Chromosomes in Neotropical Fishes

Chromosomal Distribution of Repetitive DNA Sequences Highlights the Independent Differentiation of Multiple Sex Chromosomes in Two Closely Related Fish Species

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