The preparation of serum lipoproteins labeled with radioactive cholesterol

Whereat, Arthur F.; Staple, Ezra

doi:10.1016/0003-9861(60)90571-3

Cited by 60 publications

(9 citation statements)

References 11 publications

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“…The intravenously administered cholesterol-'H was complexed to human serum lipoprotein of density 1.006-1.063 according to the method of Whereat and Staple (20). All radioactive material extracted from intimal elastin of these patients was digitonin precipitable and hence was recovered as sterol.…”

Section: Methodsmentioning

confidence: 99%

The Protein and Lipid Composition of Arterial Elastin and Its Relationship to Lipid Accumulation in the Atherosclerotic Plaque

Kramsch¹,

Franzblau²,

Hollander³

1971

J. Clin. Invest.

180

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A B S T R A C T Elastin preparations from intimal layers and the media of normal and atherosclerotic human aortae were analyzed for protein and lipid content. In atherosclerotic aortae, elastin from plaques was compared with elastin from adjacent normal appearing areas of the same aorta.Arterial elastin purified by alkaline extraction appeared to be a protein-lipid complex containing free and ester cholesterol, phospholipids, and triglycerides. The lipid component of normal arterial elastin was small (1-2%). With increasing severity of atherosclerosis, there was a progressive accumulation of lipid in intimal elastin from plaques, reaching a mean lipid content of 37% in severe plaques. The increase in the lipid content of plaque elastin preparations wvas mainly due to large increases in cholesterol, over 80% of which was cholesteryl ester. This deposition of cholesterol in plaque elastin accounted for 20-34% of the total cholesterol content of the plaque. The increased lipid deposition in plaque elastin was associated with alterations in the amino acid composition of plaque elastin. In elastin from plaque intima, the following polar amino acids were increased significantly: aspartic acid, threonine, serine, glutamic acid, lysine, histidine, and arginine; whereas, cross-linking amino acids: desmosine, isodesmosine, and lysinonorleucine were decreased significantly. The amino acid and lipid composition of elastin from normal appearing aortic areas was comparable to that of normal arterial elastin except for intimal elastin directly adjacent to and medial elastin directly below the most severe plaques.

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Section: Methodsmentioning

confidence: 99%

The Protein and Lipid Composition of Arterial Elastin and Its Relationship to Lipid Accumulation in the Atherosclerotic Plaque

Kramsch¹,

Franzblau²,

Hollander³

1971

J. Clin. Invest.

180

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“…Again, however, I must emphasize that this assumes there is no extra material thickening at the junction. Table 2 gives the stress concentration factor K. (30).…”

Section: Model For Stressmentioning

confidence: 99%

“…For purposes of our discussion I'm going to limit my presentation to in vitro data obtained in our laboratory by Scott Smith (26), even though a number of the phenomena he observed have been described by previous authors (17,18,20,21,23,27,28,29,30).…”

Section: Dr Kenyonmentioning

confidence: 99%

Dynamics of Arterial Flow

Wolf¹,

Werthessen²

1979

Advances in Experimental Medicine and Biology

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“…1 mCi/kg body weight of tritiated cholesterol in serum lipoproteins was injected in the ear veins of the rabbit. (3H)-(G) cholesterol (98% radiochemically pure) (The Radiochemical Centre, England), was incorporated into serum lipoproteins in vitro as described by Whereat and Staple (1960). 24 h later, the animals were killed by exsanguination under sodium pentobarbital anesthesia, and the aorta perfused in situ with Ringer's solution at a pressure of 100 mm Hg, until the venous return to the heart was clear.…”

Section: Methodsmentioning

confidence: 99%

Cholesterol Transfer and Content in Aortic Regions with Defined Endothelial Integrity from Rabbits with Moderate Hypercholesterolemia

Bondjers¹,

Brattsand²,

Hansson³

et al. 1976

Ann Nutr Metab

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To investigate the effect of hypercholesterolemia on cholesterol transfer and retention in the arterial wall in relationship to the endothelial cell layer, moderate hypercholesterolemia was induced in rabbits by the addition of 0.1–0.3% cholesterol in the diet. In arterial tissue with intact endothelium, hypercholesterolemia up to 400 mg% did not affect free cholesterol levels. As the uptake of free cholesterol increased in parallel to serum cholesterol, the elimination of cholesterol must also have increased. In segments with defective endothelium, the free cholesterol content increased in proportion to serum cholesterol, indicating that the potential to maintain constant free cholesterol levels was related to endothelial integrity. Tissue cholesteryl ester increased with increasing serum cholesterol regardless of endothelial integrity. However, the increase was more rapid in tissue underlying defective endothelium, suggesting that also for cholesteryl ester the intact arterial endothelium constitutes an important barrier for influx and retention.

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The preparation of serum lipoproteins labeled with radioactive cholesterol

Cited by 60 publications

References 11 publications

The Protein and Lipid Composition of Arterial Elastin and Its Relationship to Lipid Accumulation in the Atherosclerotic Plaque

The Protein and Lipid Composition of Arterial Elastin and Its Relationship to Lipid Accumulation in the Atherosclerotic Plaque

Dynamics of Arterial Flow

Cholesterol Transfer and Content in Aortic Regions with Defined Endothelial Integrity from Rabbits with Moderate Hypercholesterolemia

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