The preparation of crystalline hydroxylamine satisfactory for amino acid activation studies

Eldredge, Noreen T.; Robertson, W. W.

doi:10.1016/0003-2697(65)90330-1

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1966

2005

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Cited by 4 publications

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“…Enzyme activity based on a hydroxamate assay is also subject to errors from the high concentration of hydroxamate necessary, insensitivity of the assay, interaction of some plant extracts with the hydroxamate, and its dependence upon pH (Raacke & BovC, 1960;Stulberg & Novelli, 1962). More recently Eldredge & Robertson (1965) have described a satisfactory method for the preparation of crystalline hydroxylamine of high purity which eliminates many of its earlier disadvantages. The lack of specificity can also be misleading as kinase activity can also be recorded by the assay even though aminoacyl-s-RNA synthetases are absent (Moustafa & Petersen, 1962a).…”

Section: Reactionmentioning

confidence: 99%