The preparation of CHEMFET selective gates by thin silica layer grafting and their behaviour

Bataillard, P.; Cléchet, P.; Jaffrézic‐Renault, Nicole; Kong, Xiaoqing; Martelet, C.

doi:10.1016/0250-6874(87)80038-0

Cited by 36 publications

(25 citation statements)

References 12 publications

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“…Antigen or antibody units were immobilized at the Si/SiO 2 -interface either by covalent coupling via silanization procedure or by incorporation into a polymeric membrane. Since the SiO 2 insulating layer yields an extremely high resistance, only capacitance values were derived from the impedance measurements [51,136,137]. For example, monoclonal antibodies were covalently linked to an aminosiloxane film associated with a Si/SiO 2 surface and the antigen a-fetoprotein could be detected with a sensitivity limit of 1 ng mL À1 (ca.…”

Section: Immunosensors and Dna-sensors Based On Impedance Measurementmentioning

confidence: 99%

Probing Biomolecular Interactions at Conductive and Semiconductive Surfaces by Impedance Spectroscopy: Routes to Impedimetric Immunosensors, DNA‐Sensors, and Enzyme Biosensors

2003

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Impedance spectroscopy is a rapidly developing electrochemical technique for the characterization of biomaterialfunctionalized electrodes and biocatalytic transformations at electrode surfaces, and specifically for the transduction of biosensing events at electrodes or field-effect transistor devices. The immobilization of biomaterials, e.g., enzymes, antigens/antibodies or DNA on electrodes or semiconductor surfaces alters the capacitance and interfacial electron transfer resistance of the conductive or semiconductive electrodes. Impedance spectroscopy allows analysis of interfacial changes originating from biorecognition events at electrode surfaces. Kinetics and mechanisms of electron transfer processes corresponding to biocatalytic reactions occurring at modified electrodes can be also derived from Faradaic impedance spectroscopy. Different immunosensors that use impedance measurements for the transduction of antigen-antibody complex formation on electronic transducers were developed. Similarly, DNA biosensors using impedance measurements as readout signals were developed. Amplified detection of the analyte DNA using Faradaic impedance spectroscopy was accomplished by the coupling of functionalized liposomes or by the association of biocatalytic conjugates to the sensing interface providing biocatalyzed precipitation of an insoluble product on the electrodes. The amplified detections of viral DNA and single-base mismatches in DNA were accomplished by similar methods. The changes of interfacial features of gate surfaces of field-effect transistors (FET) upon the formation of antigen-antibody complexes or assembly of protein arrays were probed by impedance measurements and specifically by transconductance measurements. Impedance spectroscopy was also applied to characterize enzymebased biosensors. The reconstitution of apo-enzymes on cofactor-functionalized electrodes and the formation of cofactor-enzyme affinity complexes on electrodes were probed by Faradaic impedance spectroscopy. Also biocatalyzed reactions occurring on electrode surfaces were analyzed by impedance spectroscopy. The theoretical background of the different methods and their practical applications in analytical procedures were outlined in this article.

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Section: Immunosensors and Dna-sensors Based On Impedance Measurementmentioning

confidence: 99%

Probing Biomolecular Interactions at Conductive and Semiconductive Surfaces by Impedance Spectroscopy: Routes to Impedimetric Immunosensors, DNA‐Sensors, and Enzyme Biosensors

2003

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“…SiaSiO 2 structures have been the most used materials in the attempts to develop capacitive biosensors. Diot et al and Bataillard et al measured capacitance changes on SiaSiO 2 structures and concluded that it should be possible to produce biosensors by grafting a biorecognition element on such surfaces [29,30]. Silicon has the advantages of being biocompatible and of being obtainable under reproducible conditions.…”

Section: Semiconductorsmentioning

confidence: 99%

Capacitive Biosensors

2001

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“…When ionic charges are adsorbed at the functionalized surface of IS structure, the flat band potential V FB varies with the ionic concentration in the adsorbed species. The sensor sensitivity was determined by the slope of the curve giving DV FB as a function of the concentration [14]. The electrolyte chosen for these measurements was ammonium acetate (CH 3 COO À , NH 4 + ) (10 À2 M) with pH=7; as the calixarene cage is hydrophobic and the NH 4 + ion hydrophilic, the latter cannot enter the cavity of the calix [6]arene.…”

Section: Static Measurementsmentioning

confidence: 99%

EIS field effect structures functionalized by p-tert-butylcalix[6]arene for Ni2+ detection

Barhoumi¹,

Maaref²,

Mlika³

et al. 2005

Materials Science and Engineering: C

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The preparation of CHEMFET selective gates by thin silica layer grafting and their behaviour

Cited by 36 publications

References 12 publications

Probing Biomolecular Interactions at Conductive and Semiconductive Surfaces by Impedance Spectroscopy: Routes to Impedimetric Immunosensors, DNA‐Sensors, and Enzyme Biosensors

Probing Biomolecular Interactions at Conductive and Semiconductive Surfaces by Impedance Spectroscopy: Routes to Impedimetric Immunosensors, DNA‐Sensors, and Enzyme Biosensors

Capacitive Biosensors

EIS field effect structures functionalized by p-tert-butylcalix[6]arene for Ni2+ detection

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