THE PREPARATION OF 2-(ALDO-POLYHYDROXYALKYL)-BENZIMIDAZOLES¹

Abstract

Griess and Harrow (1) in 1887 studied the condensation of carbohydrates with o-phenylenediamine and were able to isolate three types of reactionproducts. When an aqueous solution of o-phenylenediamine and two equivalents of aldose was evaporated to dryness, simultaneous oxidations and condensations occurred with the formation of a Schiff base (I), oxida-

Cloning, Expression, and Characterization of Bacterial l-Arabinose 1-Dehydrogenase Involved in an Alternative Pathway of l-Arabinose Metabolism

“…L-Arabino-␥-lactone was chemically synthesized with boiling potassium arabonate in 0.2 M HCl for 5 min. Potassium arabonate was prepared by the hypoiodite-in-methanol oxidization of L-arabinose (32). A solution containing 100 mM Tris-HCl (pH 9.0), 10 mM L-arabinose, 10 mM NADP ϩ , and the purified enzyme (10 g) was incubated for 30 min at 30°C, and 100 l of this solution was then analyzed.…”

A Novel α-Ketoglutaric Semialdehyde Dehydrogenase

“…␣KGSA was synthesized from potassium arabonate by sequential reactions with L-arabonate dehydratase and L-KDA dehydratase of A. brasilense. 3 Potassium arabonate was prepared by the hypoiodite-in-methanol oxidization of L-arabinose (18). The kinetic parameters, K m and k cat values, were calculated by Lineweaver-Burk plot.…”

Eukaryotic and Bacterial Gene Clusters Related to an Alternative Pathway of Nonphosphorylated L-Rhamnose Metabolism

“…A. vinelandii was cultured at 30°C in medium (pH 7.6), containing 20.0 g of L-rhamnose, 0.5 g of yeast extract, 0. Substrates-All acid-sugars were prepared by hypoiodite-inmethanol oxidization (26) SO 4 , and the produced BaSO 4 was removed by centrifugation. The pH of the filtrate was adjusted as described above.…”