The Preparation and Properties of Porous Scaffold Made of Nano-Hydroxyapatite/Polyamide66

Abstract

In this study, we prepared porous nano-hydroxyapatite/ polyamide 66 (n-HA/ PA66) porous scaffolds by injection molding method. The morphology, macrostructure and mechanical strength of the scaffolds were characterized. Osteoblasts (OBs) derived from cranial bone of SD rats were cultured and seeded on n-HA/ PA66 scaffolds. The OB/scaffold constructs were cultured for up to 18 days and the adhesion, proliferation and osteogenic activity of OBs were observed by scanning electron microscope and detected by alkaline phosphatase activity. The results showed that the porous n-HA/PA66 porous scaffolds are biocompatible and have no negative effects on the OBs in vitro. The scaffolds fulfill the basic requirements of bone tissue engineering scaffold, and have the potential application in orthopedic, reconstructive and maxillofacial surgery areas.