The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes

Dodge, James T.; Mitchell, Carolyn D.; Hanahan, Donald J.

doi:10.1016/0003-9861(63)90042-0

Cited by 4,293 publications

(1,394 citation statements)

References 22 publications

Supporting

Mentioning

1,329

Contrasting

Unclassified

Order By: Relevance

“…The plasma and buffy coat were removed using a micropipette. The cell pellet was washed and finally suspended in isotonic phosphate buffer [24]. This constituted the erythrocyte suspension.…”

Section: Erythrocyte Separationmentioning

confidence: 99%

l-carnitine as a Potential Additive in Blood Storage Solutions: A Study on Erythrocytes

Ravikumar

Hsieh

Vani

2015

Indian J Hematol Blood Transfus

View full text Add to dashboard Cite

Erythrocytes undergo various changes during storage (storage lesion) that in turn reduces their functioning and survival. Oxidative stress plays a major role in the storage lesion and antioxidants can be used to combat this stress. This study elucidates the effects of L-carnitine (LC) on erythrocytes of stored blood. Blood was obtained from male Wistar rats and stored (4°C) for 20 days in CPDA-1 (citrate phosphate dextrose adenine) solution. Samples were divided into-(i) controls (ii) LC 10 (L-carnitine at a concentration of 10 mM) (iii) LC 30 (L-carnitine at a concentration of 30 mM) and (iv) LC 60 (L-carnitine at a concentration of 60 mM). Every fifth day, the biomarkers (haemoglobin, hemolysis, antioxidant enzymes, lipid peroxidation and protein oxidation products) were analysed in erythrocytes. Hemoglobin and protein sulfhydryls were insignificant during storage indicative of the maintenance of hemoglobin and sulfhydryls in all groups. Superoxide dismutase and malondialdehyde levels increased initially and decreased towards the end of storage. The levels of catalase and glutathione peroxidase were lower in experimentals than controls during storage. L-carnitine assisted the enzymes by scavenging the reactive oxygen species produced. Hemolysis increased in all groups with storage, elucidating that L-carnitine could not completely protect lipids and proteins from oxidative stress. Hence, this study opens up new avenues of using L-carnitine as a component of storage solutions with combinations of antioxidants in order to maintain efficacy of erythrocytes.

show abstract

Section: Erythrocyte Separationmentioning

confidence: 99%

l-carnitine as a Potential Additive in Blood Storage Solutions: A Study on Erythrocytes

Ravikumar

Hsieh

Vani

2015

Indian J Hematol Blood Transfus

View full text Add to dashboard Cite

show abstract

“…Erythrocyte membranes were prepared within 24 h essentially applying the method in [12] but using Tris buffers and ghosts were finally suspended in Tris-HC1 buffer in isotonic saline (pH 7.4). The ghost protein concentration was measured by the Lowry method [13] using crystalline bovine serum albumin as standard.…”

Section: Methodsmentioning

confidence: 99%

Erythrocyte membrane abnormalities in glucose‐6‐phosphate dehydrogenase deficiency of the mediterranean and A‐types

et al. 1981

View full text Add to dashboard Cite

“…Erythrocyte ghosts were prepared according to Dodge et al [8], with minor modifications, and resuspended in isotonic NaCl t isotonic phosphate buffer pH 6.8 (9: 1, v/v). Lucensomycin and N-acetyl-lucensomycin were a kind gift of Prof. F. Arcamone and Prof. M. Ghione of Farmitalia, Milano, Italy.…”

Section: Methodsmentioning

confidence: 99%

Fluorescence of lucensomycin upon binding to erythrocyte ghosts

Crifò¹,

Strom²,

Santoro³

et al. 1971

FEBS Letters

View full text Add to dashboard Cite

The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes

Cited by 4,293 publications

References 22 publications

l-carnitine as a Potential Additive in Blood Storage Solutions: A Study on Erythrocytes

l-carnitine as a Potential Additive in Blood Storage Solutions: A Study on Erythrocytes

Erythrocyte membrane abnormalities in glucose‐6‐phosphate dehydrogenase deficiency of the mediterranean and A‐types

Fluorescence of lucensomycin upon binding to erythrocyte ghosts

Contact Info

Product

Resources

About