The preparation and catalytically active characterization of papain immobilized on magnetic composite microspheres

Han, Lei; Wang, Jiaqiang; Chen, Lingli; Li, Xiaocong; Yi, Bin; Deng, Le

doi:10.1016/j.enzmictec.2004.03.007

Cited by 95 publications

(48 citation statements)

References 17 publications

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“…The easy dispersibility of the particles facilitates contact with the substrate and therefore alleviates mass-transfer limitations. In this regard, a number of enzymes including glucoamylase [3], chymotrypsin [4], papain [5], D-amino acid oxidase [6], and -glutamyltranspeptidase [7] have been successfully immobilized on magnetic particles.…”

Section: Introductionmentioning

confidence: 99%

Preparation of carboxylated magnetic particles for the efficient immobilization of C-terminally lysine-tagged Bacillus stearothermophilus aminopeptidase II

Huang

Wei

Yang

et al. 2010

J Ind Microbiol Biotechnol

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This article reports the synthesis and use of surface-modified iron oxide particles for the simultaneous purification and immobilization of Bacillus stearothermophilus aminopeptidase II (BsAPII) tagged C-terminally with either tri- or nona-lysines (BsAPII-Lys(3/9)). The carboxylated magnetic particles were prepared by the simple co-precipitation of Fe(3+)/Fe(2+) in aqueous medium and then subsequently modified with adipic acid. Transmission electron microscopy (TEM) micrographs showed that the carboxylated magnetic particles remained discrete and had no significant change in size after binding BsAPIIs. Wild-type enzyme and BsAPII-Lys(3) could be purified to near homogeneity by the carboxylated magnetic particles, but it was not easy to elute the adsorbed BsAPII-Lys(9) from the matrix. Free BsAPII, BsAPII-Lys(3), and BsAPII-Lys(9) were active in the temperature range 50-70 degrees C and all had an optimum of 50 degrees C, whereas the optimum temperature and thermal stability of BsAPII-Lys(3) and BsAPII-Lys(9) were improved as a result of immobilization. The immobilized BsAPII-Lys(9) could be recycled ten times without a significant loss of the enzyme activity and had a better stability during storage than BsAPII. Owing to its high efficiency and cost-effectiveness, this magnetic adsorbent may be used as a novel purification-immobilization system for the positively charged enzymes.

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Section: Introductionmentioning

confidence: 99%

Preparation of carboxylated magnetic particles for the efficient immobilization of C-terminally lysine-tagged Bacillus stearothermophilus aminopeptidase II

Huang

Wei

Yang

et al. 2010

J Ind Microbiol Biotechnol

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“…A number of methods for immobilization of enzymes have been reported in the literature and they can be divided into five basic groups: (1) covalent attachment to solid supports; (2) adsorption to solid supports; (3) entrapment within polymeric gels; (4) cross-linking to solid supports with biofunctional reagents; and (5) encapsulation of enzymes within membranes permeable only to substrates and products. Successful application of immobilization techniques to enzyme must satisfy the requirement of carriers having durability and high efficiency as well as low cost [1][2][3][4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Immobilization of β-Galactosidase onto Magnetic Beads

Zhang¹,

Gao

Gao³

2009

Appl Biochem Biotechnol

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A study of the cross-linking of beta-galactosidase on magnetic beads is reported here. The magnetic beads were prepared from artemisia seed gum, chitosan, and magnetic fluid in the presence of a cross-linking regent (i.e., glutaraldehyde). The reactive aldehyde groups of the magnetic beads allowed the reaction of the amino groups of the enzymes. The animated magnetic beads were used for the covalent immobilization of beta-galactosidase. The effect of various preparation conditions on the activity of the immobilized beta-galactosidase, such as immobilizing time, amount of enzyme, and the concentration of glutaraldehyde, were investigated. The influence of pH and temperature on the activity and the stability of the enzyme, both free and immobilized, have been studied. And o-nitrophenyl-beta-D: -galactopyranoside (ONPG) was chosen as a substrate. The beta-galactosidase immobilized on the magnetic beads resulted in an increase in enzyme stability. Optimum operational temperature for immobilized enzyme was 10 degrees Celsius higher than that of free enzyme and was significantly broader.

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“…Recently, researchers have shown that immobilization of molecular recognition elements, such as enzymes, to or within a support matrix significantly increases the enzyme activity lifetime. [95][96][97] The ability of our PVAPCCA hydrogel material to dehydrate and reversibly rehydrate enables the use of enzymes and increases its potential for commercial applications.…”

Section: Resultsmentioning

confidence: 99%

Poly(vinyl alcohol) Rehydratable Photonic Crystal Sensor Materials

Muscatello

Asher

2008

Adv Funct Materials

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We developed a new photonic crystal hydrogel material based on the biocompatible polymer poly (vinyl alcohol) (PVA), which can be reversibly dehydrated and rehydrated, without the use of additional fillers, while retaining the diffraction and swelling properties of polymerized crystalline colloidal arrays (PCCA). This chemically modified PVA hydrogel photonic crystal efficiently diffracts light from the embedded crystalline colloidal array. This diffraction optically reports on volume changes occurring in the hydrogel by shifts in the wavelength of the diffracted light. We fabricated a pH sensor, which demonstrates a 350 nm wavelength shift between pH values of 3.3 and 8.5. We have also fabricated a Pb(+2) sensor, in which pendant crown ether groups bind lead ions. Immobilization of the ions within the hydrogel increases the osmotic pressure due to the formation of a Donnan potential, swelling the hydrogel and shifting the observed diffraction in proportion to the concentration of bound ions. The sensing responses of rehydrated PVA pH and Pb(+2) sensors were similar to that before drying. This reversibility of rehydration enables storage of these hydrogel photonic crystal sensors in the dry state, which makes them much more useful for commercial applications.

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The preparation and catalytically active characterization of papain immobilized on magnetic composite microspheres

Cited by 95 publications

References 17 publications

Preparation of carboxylated magnetic particles for the efficient immobilization of C-terminally lysine-tagged Bacillus stearothermophilus aminopeptidase II

Preparation of carboxylated magnetic particles for the efficient immobilization of C-terminally lysine-tagged Bacillus stearothermophilus aminopeptidase II

Immobilization of β-Galactosidase onto Magnetic Beads

Poly(vinyl alcohol) Rehydratable Photonic Crystal Sensor Materials

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