The Preparation and Absorption Spectra of Five Pure Carotenoid Pigments

Zscheile, F. P.; White, Jonathan; Beadle, B. W.; Roach, James

doi:10.1104/pp.17.3.331

Cited by 115 publications

(43 citation statements)

References 3 publications

(4 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…27), and β-carotene at ∼425, 451, and 483 nm. 39,40 The brown and red macroalgae had distinctly different signatures from one another and from the green algae. Once again, a distinct absorption maximum near 675 nm is conditioned by chlorophyll a absorption.…”

Section: Discussionmentioning

confidence: 99%

In-air spectral signatures of the Baltic Sea macrophytes and their statistical separability

Kotta

Remm

Vahtmäe

et al. 2014

J. Appl. Remote Sens

View full text Add to dashboard Cite

Abstract. Many macroalgal species potentially have distinctive spectral signatures detectable using remote sensing. In order to map the spatial distribution of these species, their spectral properties have to be quantified and statistical differences between species need to be assessed. In the present study, we collected a spectral library of the key benthic macrophyte species in the Baltic Sea area and presented the methodology that allows quantifying statistical differences between their reflectance spectra. The results indicate that three broad groups of algaegreen, brown, and red algae-can be separated based on their optical signatures. In general, the between-species differences are too small to allow easy recognition of benthic algae based on their untransformed reflectance spectra. However, the distinctness of the studied species and taxa improves if standardized reflectance values are used. The best indicative spectral range was at 530 to 570 nm for the separation of species and of larger taxonomic units. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

show abstract

Section: Discussionmentioning

confidence: 99%

In-air spectral signatures of the Baltic Sea macrophytes and their statistical separability

Kotta

Remm

Vahtmäe

et al. 2014

J. Appl. Remote Sens

View full text Add to dashboard Cite

show abstract

“…Each eluted pigment was drawn into a syringe, filtered as described above into chilled Eppendorf tubes (1.5 mL), bubbled with nitrogen gas, capped, and kept in a darkened ice bucket. The concentration of each pigment was determined spectroscopically at the wavelength for each pigment's absorption maximum using previously established extinction coefficients (Gillam 1935;MacKinney 1940;Zscheile et al 1942;Goodwin 1955;Davies 1965Davies , 1976Hager and Meyer-Bertenrath 1966;Liaaen-Jensen and Jensen 1971;Stransky 1978), after which the samples were bubbled with nitrogen gas in autosampler vials, sealed, and kept on ice/ refrigerated in darkness until injection into the HPLC system (three different volumes of each pigment sample) in order to provide the calibration for each pigment.…”

Section: Leaf Pigments and Tocopherolsmentioning

confidence: 99%

Differences in light-harvesting, acclimation to growth-light environment, and leaf structural development between Swedish and Italian ecotypes of Arabidopsis thaliana

Stewart

Cohu

Polutchko

et al. 2015

Planta

View full text Add to dashboard Cite

Leaf morphological differences have an impact on light distribution within the leaf, photosynthesis, and photoprotection in Arabidopsis thaliana ecotypes from near the limits of this species' latitudinal distribution in Europe. Leaf morphology, photosynthesis, and photoprotection were characterized in two Arabidopsis ecotypes from near the limits of this species' latitudinal distribution in Europe (63°N and 42°N). The Swedish ecotype formed thicker leaves and upregulated photosynthesis more substantially than the Italian ecotype in high-light environments. Conversely, the smaller rosette formed, and lesser aboveground biomass accumulated, by the Swedish versus the Italian ecotype in low growth-light environments is consistent with a lesser shade tolerance of the Swedish ecotype. The response of the thinner leaves of the Italian ecotype to evenly spaced daily periods of higher light against a background of otherwise non-fluctuating low light was to perform the same rate of photosynthesis with less chlorophyll, rather than exhibiting greater rates of photosynthesis. In contrast, the thicker leaves of the Swedish ecotype showed elevated photosynthetic performance in response to daily supplemental higher light periods in a low-light growth environment. These findings suggest significant self-shading in the lower depths of leaves of the Swedish ecotype by the chloroplasts residing in the upper portions of the leaf, resulting in a requirement for higher incident light to trigger photosynthetic upregulation in the lower portions of its thicker leaves. Conversely, photoprotective responses in the Italian ecotype suggest that more excess light penetrated into the lower depths of this ecotype's leaves. It is speculated that light absorption and the degree of utilization of this absorbed light inform cellular signaling networks that orchestrate leaf structural development, which, in turn, affects light distribution and the level of absorbed versus photosynthetically utilized light in a leaf.

show abstract

“…They did not determine the ranges of these calculated values. This study also included a few additional studies (Miller, 1937; Zscheile et al, 1942) examining the mass-specific absorption coefficients for α-, β-carotene, lycopene, xanthophyll, and zeaxanthin (Table 4). Mass-specific absorption and absorption coefficients for non-algal optically active constituents have also been determined (Table 4).…”

Section: Introductionmentioning

confidence: 99%

Determination of absorption coefficients for chlorophyll a, phycocyanin, mineral matter and CDOM from three central Indiana reservoirs

Nguy-Robertson

Tedesco

et al. 2013

Journal of Great Lakes Research

View full text Add to dashboard Cite

The Preparation and Absorption Spectra of Five Pure Carotenoid Pigments

Cited by 115 publications

References 3 publications

In-air spectral signatures of the Baltic Sea macrophytes and their statistical separability

In-air spectral signatures of the Baltic Sea macrophytes and their statistical separability

Differences in light-harvesting, acclimation to growth-light environment, and leaf structural development between Swedish and Italian ecotypes of Arabidopsis thaliana

Determination of absorption coefficients for chlorophyll a, phycocyanin, mineral matter and CDOM from three central Indiana reservoirs

Contact Info

Product

Resources

About