The preovulatory rise of ovarian ornithine decarboxylase is required for progesterone secretion by the corpus luteum

Bastida, C.M.; Tejada, Francisco; Cremades, Adolfo Díaz-Bautista; Peñafiel, Rafael

doi:10.1016/s0006-291x(02)00191-2

Cited by 19 publications

(13 citation statements)

References 34 publications

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“…OAT activity in the porcine placenta was elevated during early pregnancy, reflecting the synthesis of polyamines essential for conceptus development through ornithine decarboxylase (ODC), a key enzyme in polyamine biosynthesis (Wu et al 2004(Wu et al , 2005. Inhibition of the preovulatory rise of ovarian ODC leads to a decrease in CL P 4 synthesis during the murine oestrous cycle (Bastida et al 2002). In our study, OAT was most abundant in CL during early pregnancy.…”

Section: Protein Synthesismentioning

confidence: 55%

Ovine corpus luteum proteins, with functions including oxidative stress and lipid metabolism, show complex alterations during implantation

Arianmanesh¹,

McIntosh²,

Lea³

et al. 2011

Journal of Endocrinology

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Progesterone (P 4 ) secreted by the corpus luteum (CL) is critical for in utero embryo survival and development, although CL proteins are key regulatory factors during the luteal phase. We, therefore, characterised protein expression patterns in ovine CL of pregnancy (days 12, 16 and 20) compared with those of controls, CL of oestrous cycle (days 12 and 16), using two-dimensional gel electrophoresis (2DE) gel-based proteomics. Proteins in 24 significantly altered spots were identified by tandem mass spectroscopy. At the time of embryo implantation (day 16), 77 spots were up-regulated and 101 spots were down-regulated in CL of pregnancy compared with regressed CL. Vimentin, lamin A/C (LMNA), [Mn] superoxide dismutase (SOD2), isocitrate dehydrogenase 1, annexin A1 and elongation factor Tu, mitochondrial (TUFM) altered during CL regression, whereas glutathione S-transferase A1, apolipoprotein A-1, myxovirus resistance protein 1, ornithine aminotransferase and enoyl-CoA hydratase, mitochondrial (ECHS1) tended to be altered during CL maintenance. biliverdin reductase B (BLVRB), FDXR, guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2 (GNB2) and cytochrome bc1 complex subunit 1, mitochondrial (UQCRC1) showed divergent expression during CL regression and maintenance. The expression of two representative proteins, SOD2 and BLVRB, by western blot increased in CL of non-pregnant ewes on day 16 compared with that on day 12. SOD2 and BLVRB were localised in the large and small luteal cells and endothelial cells of CL over peri-implantation periods. 2DE gel and mass spectrometry have been used, for the first time, to study ovine CL function. We have identified proteins involved in key pathways, including oxidative stress, steroidogenesis, signal transduction and apoptosis, which have not previously been associated with changes occurring in the CL during the peri-implantation period. These proteins are most likely involved with mechanisms allowing the CL to produce P 4 during early pregnancy.

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Section: Protein Synthesismentioning

confidence: 55%

Ovine corpus luteum proteins, with functions including oxidative stress and lipid metabolism, show complex alterations during implantation

Arianmanesh¹,

McIntosh²,

Lea³

et al. 2011

Journal of Endocrinology

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“…Furthermore, the inhibition of LHinduced stimulation of ODC activity by diXuoromethylornithine (DFMO, an irreversible inhibitor of ODC activity) collapsed the production of progesterone in mouse corpus luteum (Bastida et al 2002). These observations allow us to speculate that the regulation of ODC by AZIN2 in these Fig.…”

Section: Discussionmentioning

confidence: 85%

“…An intact ODC activity is needed for normal folliculogenesis and luteinization in mouse ovary (Bastida et al 2005). Moreover, blocking of the preovulatory rise of ODC activity by in vivo treatment with diXuoromethylornithine (DFMO), a speciWc inhibitor of ODC, abolishes the secretion of progesterone by the corpus luteum (Bastida et al 2002). The gonadotropin-induced activation of ODC in gonads suggests a role for ODC in the regulation of steroid hormone secretion.…”

Section: Introductionmentioning

confidence: 99%

High expression of antizyme inhibitor 2, an activator of ornithine decarboxylase in steroidogenic cells of human gonads

Mäkitie

Kanerva

Sankila

et al. 2009

Histochem Cell Biol

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High activity of ornithine decarboxylase (ODC), the rate-limiting enzyme of polyamine synthesis, is typically present in rapidly proliferating normal and malignant cells. The mitotically inactive steroidogenic cells in rodent testis and ovaries, however, also display high ODC activity. The activity of ODC in these cells responds to luteinizing hormone, and inhibition of ODC reduces the production of steroid hormones. Polyamines and ODC also control proliferation of germ cells and spermiogenesis. The activity of ODC, especially in proliferating cells, is regulated by antizyme inhibitor (AZIN). This protein displaces ODC from a complex with its inhibitor, antizyme. We have previously identified and cloned a second AZIN, i.e. antizyme inhibitor 2 (AZIN2), which has the highest levels of expression in brain and in testis. In the present study, we have used immunohistochemistry and in situ hybridization to localize the expression of AZIN2 in human gonads. We found a robust expression of AZIN2 in steroidogenic cells: testicular Leydig cells and Leydig cell tumors, in ovarian luteinized cells lining corpus luteum cysts, and in hilus cells. The results suggest that AZIN2 is not primarily involved in regulating the proliferation of the germinal epithelium, indicating a different role for AZIN1 and AZIN2 in the regulation of ODC. The localization of AZIN2 implies possible involvement in the gonadal synthesis and/or release of steroid hormones.

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“…Although it has been known for many years that pituitary and gonadal hormones induce ODC in target tissues (2,24,59), the physiological function of ODC induction in response to these factors still remains largely unknown. In this respect, we (7,8) recently found that the induction of ovarian ODC mediated by luteinizing hormone is necessary for folliculogenesis and luteinization. These studies suggested that ODC induction may be relevant for the regulation of the steroidogenic activity in the mouse ovary.…”

mentioning

confidence: 98%

Sexual dimorphism of ornithine decarboxylase in the mouse adrenal: influence of polyamine deprivation on catecholamine and corticoid levels

Bastida¹,

Cremades²,

Castells³

et al. 2007

American Journal of Physiology-Endocrinology and Metabolism

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Bastida CM, Cremades A, Castells MT, López-Contreras AJ, López-García C, Sánchez-Mas J, Peñ afiel R. Sexual dimorphism of ornithine decarboxylase in the mouse adrenal: influence of polyamine deprivation on catecholamine and corticoid levels. Am J Physiol Endocrinol Metab 292: E1010 -E1017, 2007. First published December 5, 2006; doi:10.1152/ajpendo.00316.2006.-Adrenal sexual dimorphism is thought to be important in explaining sex-related differences regarding prevalent diseases and the responses to stress and drugs. We report here that in CD1 mice there is marked sexual dimorphism affecting not only gland size and corticoid hormone secretion but also adrenal ornithine decarboxylase (ODC), polyamine, and catecholamine levels in which testosterone appears to be a major determinant. Our results show that adrenal weight, ODC activity, and corticosterone and aldosterone secretion were higher in female than in male mice and that orchidectomy brought these male parameters closer to the values found in females. mRNA levels of steroidogenic proteins SF-1, Dax-1, steroid 21-hydroxylase, and aldosterone synthase appeared to be slightly higher in female than in male adrenals. Immunocytochemical analysis of adrenal ODC revealed that immunoreactivity was higher in females than in males and was located mainly in the cortical cells, and especially in zona glomerulosa, whereas no sex differences in ODC mRNA levels were observed. These results suggest that sex-associated differences in the expression of ODC in the mouse adrenal gland appear to be related mainly to posttranscriptional mechanisms. Combination treatment of mice with ␣-difluoromethylornithine (a suicide inhibitor of ODC) and a polyamine-deficient diet produced a marked decrease in adrenal polyamine and catecholamine levels and a significant reduction in plasma corticosterone and aldosterone concentrations that were not associated with a decrease in the mRNA levels of steroidogenic proteins. All of these data suggest a relevant role for testosterone, ODC, and polyamines in the mouse adrenal function. mice; ␣-difluoromethylornithine; corticosterone; aldosterone; steroidogenesis THE ADRENAL GLAND plays an important role in stress response, with the hypothalamic-pituitary-adrenal axis and the sympatho-adrenomedulary system as the principal components (41). Considerable evidence has accumulated indicating that gonadal factors act as regulators of the hypothalamic-pituitary-adrenal axis and adrenal function. In the rat there is a marked sexual dimorphism in adrenal weight, plasma ACTH, and corticosterone concentrations, with females having higher values than males (1,25,27,30,45). This adrenal sexual dimorphism is thought to be an important factor in explaining sex differences in relation to prevalent diseases associated with hypothalamic-pituitary-adrenal axis reactivity, such as cardiovascular disease, inflammation, diabetes, and hypertension (19,55). On the other hand, it has been suggested that a transient increase in ornithine decarboxylase (ODC) activity and tissue p...

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The preovulatory rise of ovarian ornithine decarboxylase is required for progesterone secretion by the corpus luteum

Cited by 19 publications

References 34 publications

Ovine corpus luteum proteins, with functions including oxidative stress and lipid metabolism, show complex alterations during implantation

Ovine corpus luteum proteins, with functions including oxidative stress and lipid metabolism, show complex alterations during implantation

High expression of antizyme inhibitor 2, an activator of ornithine decarboxylase in steroidogenic cells of human gonads

Sexual dimorphism of ornithine decarboxylase in the mouse adrenal: influence of polyamine deprivation on catecholamine and corticoid levels

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